...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...mitochondria-targeting sequence makes Keima useful for monitoring mitophagy. It’s important to remember that because...Maria Cuervo, and Per O. Seglen. "Methods for monitoring autophagy from yeast to human." Autophagy 3.3...PMC4517063. Yoshii, Saori R., and Noboru Mizushima. "Monitoring and measuring autophagy." International journal...

...Willoughby   Using RUBY as a selection marker The creators of RUBY highlight its use in crop plants under... is difficult for new members of the lab or collaborators unfamiliar with these mutant plants. Using RUBY...promoter with self-cleaving 2A site linkages and a terminator. Image from He, Y., Zhang, T., Sun, H. et al...., Sun, H. et al. A reporter for noninvasively monitoring gene expression and plant transformation. Hortic...Transformation CRISPR 101: CRISPR-Mediated Plant Base Editors...

...model for cancer-specific research. Membrane receptors and ion channels are often exogenously expressed... degrade over time in culture. This can affect factors that could skew research results (gene expression... Especially if your research interests include receptor biology, ion channels, or involve human protein...Platform to Produce Recombinant Proteins and Viral Vectors. Front Bioeng Biotechnol. (2021) (9):796991. DOI...10 Tips for Mammalian Cell Culture Adenoviral Vector Production and Troubleshooting Additional resources...

...different story altogether? The unfortunate answer is yes, largely due to a few limiting factors.  To start...spent your life making connections and finding mentors -- well, it's time you call upon these people for... is more important than the product itself -- investors would rather fund excellent people with mediocre...Harvard/MIT faculty, venture capital and angel investors, and business development heads at major companies...

...pitfalls: they require construction of plasmid vectors and/or rely on cumbersome in vitro synthesis of... have low knock-in efficiency. Together, these factors prevent stem cell researchers from generating clonal...purchased and there is no need to construct plasmid vectors or synthesize/purify IVT sgRNA in the lab. Figure...experiments. Notably, we could tag >60 transcription factors in a short (3-week) time window; this was possible...csRNP experiments, it can be ordered in bulk and stored in small aliquots at -80 oC. Assemble csRNP: Anneal...success of knock-in experiments depends on many factors. Some of these include: Most importantly, the...

...looping factors developed thus far for these latter experiments, such as transcription factors tethered...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor, sufficiently suppresses basal degradation of ...neurons. Earlier this year, the Hegemann lab and collaborators report their discovery of a new family of seven...

...one are in a pLVX vector background, so you can package them into lentiviral vectors if you need to. Additionally...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...It’s also important to determine if the human interactors we found in our map have an impact on virus replication... replication. While we and our collaborators are starting these experiments, there's so much to do that...

...researchers, it can expose them to a new institute and mentor that they may want to consider for future work ...professor or institute, ask if you will have a bench mentor or anyone else available to directly help you during... during your visit. Reach out to your bench mentor (if you have one) to communicate your plan as well.... comfortable initiating this conversation, your mentor can help get the ball rolling! If you are applying...techniques. Show up on time (don’t make your bench mentor wait on you!), make sure you are prepared (read...

...elicit an immune response from the body. Several factors contribute to the immunogenicity of a drug, including...these algorithms to model individually-specific factors, such as previous exposures to that specific antigen...assessments, as well as analyze the contributing factors that in silico prediction algorithms fail to capture...10.1634/theoncologist.2016-0061WHO. International monitoring of adverse reactions to drugs: adverse reaction...WHO Collaborating Centre for International Drug Monitoring, Uppsala, Sweden, 1992. Mattei, A. E., Gutierrez...

...will choose to start their own academic research laboratory. How does one even go about starting a lab? Obviously... does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager, researcher, adviser...experiments and grant writing. You have to enjoy the mentoring role.” She points out that new PIs may not recognize...student or postdoc may require different types of mentoring. Some mentees prefer lots of advice and micromanagement...tips for starting a lab. Or share your success stories below. And don't forget to stay tuned for the last...