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We narrowed to 818 results for: cell

Showing: 491 - 500 of 818 results

  1. Antibodies 101: Conjugation

    Type
    Blog Post
    ...and Protein Modifications in Cells and Tissues in Situ. Curr Protoc Cell Biol 89:e115. https://doi.org... like proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et...epitope and transcriptome measurement in single cells. Nat Methods 14:865–868. https://doi.org/10.1038...

  2. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...slightly more efficient and less toxic to mammalian cells (Allen, et al., Frontiers in Genome Editing).  Some...enhance CRISPR-Cas genome editing in human primary cells. Nat Biotechnol 33, 985–989 (2015). https://doi....Enhance CRISPR Genome Editing Systems in Mammalian Cells. Frontiers in Genome Editing, 28 (2021). https:/...deactivation with photocleavable gRNAs. Molecular Cell 81, 7, 1553-1565 (2021). https://doi.org/10.1016...evolved ways to prevent degradation of important cellular RNA molecules with post translational modifications...transfection efficiency and even track localization in cellular experiments. There are many suitable dyes to choose...

  3. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...in transfected eukaryotic cells, allowing for them to be used as intracellular inhibitors.  Aptamers are...small enough to be expressed in bacterial and other cell systems. Since the majority of these alternative...the constant region. Nonspecific binding, caused cell receptors for the constant region, is also reduced..., peptides, amino acids, drugs, metal ions, and cells. They can be used either with or as fluorophores... may sound. Validated antibodies are still an excellent option for many applications, and frankly, if ...

  4. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

    Type
    Blog Post
    ... be expressed in the same cell, but not necessarily at the same subcellular compartment as the opsin or...modulate the activity of neurons and other brain cells. Since the opsins or chemogenetic receptors used...neuropil conceals identification of opsin-expressing cells). For other things to keep in mind when making fluorescent...cerevisiae by use of an epitope addition method. Mol Cell Biol 8:2159–2165 . https://doi.org/10.1128/mcb.8.5.2159...

  5. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...delivered using AAV injection, the majority of the cells surrounding the injection site will become infected... ensure that the reporter remains silent until a cell or tissue-specific Cre is provided. Since the FLEx...used to study the physiology or behavior of certain cell populations.  Acknowledgement:Through a partnership...FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping...for monitoring Cre-mediated recombination at the cellular level in the mouse. 2003 PubMed PMID: 12665802...

  6. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...plasmids to upregulate their expression in specific cells. With a more concrete understanding of the molecular...molecules targeting SMN1 and its partners in mouse cell lines. Dr. Di Schiavi hopes that these studies will... of ~30 patient derived peritoneal mesothelioma cells lines and assess their tumorigenesis. These experiments...

  7. Lentivirus Plasmids

    Type
    Collection
    ...Lentivirus can transduce both dividing and non-dividing cells without a significant immune response. These viruses... in the viral particle) is delivered to a target cell upon infection. When lentivirus is used for research...the researcher wants delivered to specific target cells. This genome is encoded by plasmids called "transfer...encoded by the transfer plasmid is toxic to mammalian cells, it can be difficult to efficiently produce viral...

  8. Feng Zhang Multiplexed Overexpression of Regulatory Factors (MORF) Collection

    Type
    Collection
    ...Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link opens in...be used for screening transcription factors for cellular programming or cancer drug resistance. Depositor...transcription factors for applications such as cellular programming or cancer drug resistance. Protocols...

  9. Worm Expression Resources

    Type
    Collection
    ...Worms are particularly well suited for studies in cell biology, development, and aging. Scientists can .... elegans hermaphrodite consists of 959 somatic cells, which makes them attractive for imaging and fluorescence...researchers to express desired constructs in precise cell types and/or developmental timeframes. Here we highlight...

  10. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...e.g., pHelper) and specific packaging cell lines (e.g., HEK293 cells) during AAV production. Read our AAV...AAV can transduce both dividing and non-dividing cells with a low immune response and low toxicity. Although...
Showing: 491 - 500 of 818 results