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We narrowed to 971 results for: lars;

Showing: 491 - 500 of 971 results

  1. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...clustered regularly interspaced palindromic repeats) Cas (CRISPR associated genes) system. This popular genome...polythetic classification based on evolutionary similarity (5). Type II CRISPR/Cas is the system that scientists...translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR systems between and ...

  2. Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools

    Type
    Blog Post
    ... by Torsten Wittmann’s lab at UCSF to modulate cellular dynamics with light by modifying EB1. EB1 normally... opens novel ways in manipulating and studying cellular dynamics. The photoinducible control of protein...potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature Cell Biology...the base editing podcast segment The increasing popularity of single base-editors has resulted in the rapid...

  3. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...to learn much more about cellular DNA repair pathways. An emphasis on cellular repair post-CRISPR cleavage...Twitter if you missed our live updates! My most popular tweet from the conference is shown above. The blog..., which alter genomic DNA without the need for cellular HDR.   Having had time to digest my notes, since...

  4. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella. When fused ...biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein,...Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast." ...measuring autophagy." International journal of molecular sciences 18.9 (2017): 1865.  PubMed PMID: 28846632...

  5. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...talk about Z DNA that inspired her to major in molecular biology in college. She then pursued a PhD in ...not only got incredibly compelled by wet bench molecular research, but also became passionate about virology...of Viral Vectors Karen Guerin for the journal Molecular Therapy. Vandenberge is also the Grousbeck Family... Gene Therapy Center, Associate Director of the Ocular Genomics Institute, and an Associate Member at ...

  6. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...If you work in a lab that regularly does immunoimaging, there’s likely a large collection of antibodies...specific to denatured versions, or even to just that particular assay.      Fig. 1: A native protein ...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...

  7. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type
    Blog Post
    ...their name? In 1952, Joshua Lederberg set out to clarify the classification of these cytoplasmic inheritance...began to study other extrachromosomal particles, particularly Resistance or R-factors. Like F-factors, R-factors...especially lambda, was the tool of choice for molecular biologists wanting to study bacterial genetics...pSC101 became the first plasmid cloning vector and molecular biology was never the same. Over the next few ...

  8. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...meetings or calls regularly and don’t cancel, so that you drive progress with regular accountability checks...Mentoring for Scientists Guide.  You can use it to clarify and discuss values. The activity is just the start...Relevant, and Time-bound. It is a good idea to take larger goals (e.g. Figure out what I want to do after ...

  9. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...drastically alter your strategy. I realized that similar types of thinking go into developing gene therapy...PLASMID is a game where you need to apply basic molecular biology, cell biology, microbiology, genetic engineering... He is also founder and consultant at FRESCI particularly interested in advanced in vitro models technology...Resource Center Resources on Addgene.org Find molecular biology educational resources Find protocol videos...

  10. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...FPs are tremendously complicated entities with a large range of characteristics (color, brightness, photostability... a project written in Python & Django, using a popular database management system (PostgreSQL), that aims...use case for FPbase is finding information on a particular FP; this can be done directly using the search...your help!), I believe this represents one of the largest available online collections of FP spectra. Every...
Showing: 491 - 500 of 971 results