We narrowed to 997 results for: lars;

...double-strand break (DSB). DSBs can be caused by intracellular factors such as nucleases and reactive oxygen...vertically at the orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing...thus dsDNA plasmids are generally preferred for large insertions such as fluorescent proteins or selection...arms between 500-1000 bps. HDR efficiency with circular plasmid templates is generally low; to increase...cerevisiae. Paques F and Haber JE. Microbiology and Molecular Biology Reviews (1999). PubMed. The Road Less ...

...landmark discovery in the 1990s and quickly rose to popularity. Compared to CRISPR, RNAi methods are often quicker...blog, we’ll be focusing on shRNA, one of the most popular choices for gene silencing. RNA interference RNAi...degradation of RNA-induced silencing complex. Molecular Cell, 82(1), 30–43. https://doi.org/10.1016/j.... and assessment of gene knockdown. Methods in Molecular Biology, 139–156. https://doi.org/10.1007/978-...on addgene.org Mammalian shRNA Tools for RNAi Molecular Biology Reference ...

...commonly cited that approximately one-third of cellular proteins are modified through phosphorylation ...generation of pSer-incorporated proteins requires a molecular toolkit containing the OTS, an appropriate bacterial...using the same expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation...Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of cell signaling...Tomida, J., et al. "Detection of phosphorylation on large proteins by Western blotting using Phos-tag containing...

...Lichtman, both of Harvard’s Department of Molecular and Cellular Biology, harnessed these attributes of ...complementary Flpbow construct that is functionally similar to Cre-based Brainbow, but is controlled by FLP...as connections between motor neurons and the neuromuscular junction. In zebrafish, the method has become...neural expression patterns. Hampel S, Chung P, McKellar CE, Hall D, Looger LL, Simpson JH. Nature Methods...

...hard work and talent for many years (at a very low salary I might add). You have a right and responsibility...are always traveling, or too busy to interact regularly with the newer lab members. You must get an idea... the lab head? Are meetings with the lab head regularly scheduled or only ad hoc? Does the mentor keep... and learn. Graduate students and postdocs in particular must care about this. The training you get now...at your data and suggest follow up experiments regularly? Can you work on some experiments of your own ...

...therefore determines the timing of gene expression and largely defines the amount of recombinant protein that ...cumate-inducible gene expression system, which functions in a similar manner to the Tet system. The transactivator configuration...interrogate the activity of uncharacterized promoters. Similarly, placing UAS upstream of a transgene permits directed... binary systems above if they all behave very similarly?” The simple answer is that sometimes it is necessary...systems. Recent work has even used all 3 in a single modular vector for ease of use! Conclusion This post contains...

...plasmid molar ratio you would like to use for your AAV packaging transfection. In my lab we regularly use ...requires transfecting three plasmids at specific molar ratios. To get these ratios right, you need to do... Vitro and In Vivo Use." Current Protocols in Molecular Biology (2007): 16-25. PubMed PMID: 18265393. ...

...Choice" series. Each month, I'll summarize the most popular post of the month and point out one or more additional... ranging from new tools you can use to monitor cellular perturbations to how CRISPR has made it much easier...surrounding anything to do with CRISPR, our most popular post in July (with over 700 views) was an update...showing that Cpf1 has lower off-target editing and similar on-target editing when compared to the most commonly...

...Bengyella’s work has focused on elucidating the molecular mechanisms behind interactions between crops like...around the world. Kwabena O. Duedu Dr. Duedu’s molecular biology work has focused on using various cloning...plasmids he acquires through Addgene to produce molecular biological tools like Taq polymerase, T7 polymerase...heavy focus on teaching. He hopes to teach his molecular biology students about the functions of these ...

...indicates that the amount of leaky expression can largely be attributed to the level of spontaneous recombination...) promoter for catecholamine neurons or glial fibrillary acidic protein (GFAP) for astrocytes it is important...tested in the researcher's system before beginning large scale experiments. This will help to ensure the ...10.1371/journal.pone.0076310 Burger C, Gorbatyuk OS, Velardo MJ, Peden CS, Williams P, Zolotukhin S, Reier PJ...Different Regions of the Central Nervous System. Molecular Therapy 10:302–317. https://doi.org/10.1016/j....