We narrowed to 1,104 results for: Ide

...messengers. Optical control of kinase activity can provide greater spatiotemporal resolution than pharmacological...vanishing, and pa= photoactivation) domains on either side of the active site of a constitutively active MEK1...

... Kramer L, Campbell RE. Structure-and mechanism-guided design of single fluorescent protein-based biosensors...1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral...

...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein ... if that’s the option you choose. Once you’ve identified the linear portion of your standard curve, you...

... excited about. Following scientists in fields outside your main research focus lets you keep a pulse ...window of time for publication. To help you quickly identify papers with greater influence, the average number...

...leadership and management skills. The NPA brought outside experts and many hands-on- and role-playing exercises...with other leaders in my research field should I decide to remain here.  In a time when it is so important...

...stranded break (DSB) at a genomic site specified by a guide RNA (gRNA) sequence. Users can design gRNAs to induce...SapTrap, scientists will be able to generate genome-wide libraries containing fluorescently-tagged proteins...

...strains on the blog, and also recommend this handy guide from OpenWetWare for a more extensive listing of...often ambiguous and should be viewed more as a guideline, since they are typically based on the copy number...

...plasmids tend to be high copy number. Here, we will consider the replication mechanism of plasmids under stringent...plasmid to balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled...

...expression. Take a look at all of the options and decide what might work best for your experiments! This...Additional Resources on the Addgene Blog Quick guide to working with Drosophila Terminators and PolyA...

...orange and red fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns...bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Reports 7, 42398. PubMed PMID...

...channel for more helpful animations, useful protocol videos, and quick tips for lab success.  ...

...quest, which led us to Seqera Labs, a company that provides open source workflow orchestration software for...

...working with viral vectors, but what we lacked were videos guiding completely new members of the lab or those...

...Antibody, Addgene gave to me…  An online Antibody Guide!    🎶On the fourth day of Antibody, Addgene gave...

...decision between some meaningful names…. Watch the video to find out the final four! (Note: For cases where...

... a Graduate Lab and Project Check Out 5 Science Videos from Tom McFadden Resources at Addgene.org Sign...

... a Graduate Lab and Project Check Out 5 Science Videos from Tom McFadden Resources at Addgene.org Sign...

...memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured over the...

...in the scientific community. Addgene has been considering a gradual move off X as a response to major platform...

...the 2nd day of CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with...