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We narrowed to 1,106 results for: Ide

Showing: 981 - 1000 of 1106 results

  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...messengers. Optical control of kinase activity can provide greater spatiotemporal resolution than pharmacological...vanishing, and pa= photoactivation) domains on either side of the active site of a constitutively active MEK1...

  2. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...the respective hairpin binding domain. Pairs of identical hairpin sequences specify the primary colors blue... colors and a white composite. gRNAs with two identical hairpin binding domains produce blue, green, or...

  3. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...synthesized endogenous proteins in situ. Now FUNCAT-PLA provides both quantitative and spatio-temporal information...protein synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of...

  4. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ... excited about. Following scientists in fields outside your main research focus lets you keep a pulse ...window of time for publication. To help you quickly identify papers with greater influence, the average number...

  5. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... Kramer L, Campbell RE. Structure-and mechanism-guided design of single fluorescent protein-based biosensors...1057-65. Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral...

  6. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein ... if that’s the option you choose. Once you’ve identified the linear portion of your standard curve, you...

  8. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...stranded break (DSB) at a genomic site specified by a guide RNA (gRNA) sequence. Users can design gRNAs to induce...SapTrap, scientists will be able to generate genome-wide libraries containing fluorescently-tagged proteins...

  9. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...strains on the blog, and also recommend this handy guide from OpenWetWare for a more extensive listing of...often ambiguous and should be viewed more as a guideline, since they are typically based on the copy number...

  10. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...plasmids tend to be high copy number. Here, we will consider the replication mechanism of plasmids under stringent...plasmid to balance the total plasmid number.  Let’s consider pSC101. This plasmid is stringently controlled...

  11. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...expression. Take a look at all of the options and decide what might work best for your experiments! This...Additional Resources on the Addgene Blog Quick guide to working with Drosophila Terminators and PolyA...

  12. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...orange and red fluorescence. The paper reports a wide variety of applications of the Enhanced Nano-Lanterns...bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. Reports 7, 42398. PubMed PMID...

  13. New Antibody Animation!

    Type
    Blog Post
    ...channel for more helpful animations, useful protocol videos, and quick tips for lab success.  ...

  16. The Twelve Days of Antibodies

    Type
    Blog Post
    ...Antibody, Addgene gave to me…  An online Antibody Guide!    🎶On the fourth day of Antibody, Addgene gave...

  20. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...memories of Addgene's past. Framed photos and slideshows shared some of the moments we captured over the...
Showing: 981 - 1000 of 1106 results