...for you to find the plasmids you need. We'll be monitoring and improving the maps further after this initial...SnapGene powered plasmid maps at Addgene  Three factors make our SnapGene powered plasmid maps far easier...all available sequence information from Addgene depositors and our own sequencing results: On this page ...sequence in your sequence viewer of choice. If the depositor provided a custom annotated GenBank file, this...updates will make it much easier to browse the repository and quickly identify the plasmids and features...

... are three main pathogen clearing functions or effector functions of the constant regions of antibodies...functions: Recruitment of immune cells that express receptors that recognize the Fc portion of different antibody...domains, or Fc region, can be bound by special receptors that transport antibodies through cells and into...role in allergies. They are found bound to IgE receptors on the surface of basophils and mast cells, two...of an immune response since it also changes the effector function of the antibody. Isotype switching is...

...technology or as a therapeutic.  A (very) brief history GCE came onto the synthetic biology scene in 1989...be individually optimized for each of the three factors: the ncAA, the tRNA, and the tRNA synthetases. ...balance can be extremely tricky to find: each of the factors can negatively impact both translation and fidelity...complicated.” And Ryan Mehl would know. He’s the director of GCE4All, an NIH-funded center located at Oregon... V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry, 60(46), 3455–3469...

...don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...professional network such as co-workers, professors, and mentors. Ask for specific guidance on grammatical errors...notice within 24 hrs, send a note to the program coordinator.   6. Virtual interview prep Prepare for your...practice a set of common questions with a peer or mentor. Several days prior to the interview, inspect your...Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard...

...biological systems. Size is also a factor; the pol lll promoter and terminator need be encoded only once, and...containing the RNA pol III promoter, gRNA and pol III terminator. These relatively large cassettes (considering...gRNAs that can be packaged together in a single vector. In addition, the pol III promoter is relatively...deletions of other regions, such as noncoding RNAs or regulatory elements, to examine their function. Bacteria...

...expressed in a single vector, pCRISPRainbow-DONOR1, with dCas9 supplied by a separate vector. Figure ...the 4D nucleome. Visualizing specific loci has historically been difficult, as techniques like fluorescent...technique was developed in the lab of Addgene depositor Stanley Qi and used in Chen et al. to track telomere...given chromosome simultaneously, they could also monitor the spatial relationship of the two genes over ...

...contributed by guest blogger, Gary McDowell, executive director of Future of Research. On December 1st 2016, many...of Information Act (FOIA) requests for their postdoctoral salary information as of December 1st. Salary...are an important step forward in helping the postdoctoral population continue to pursue academic work,...of the scientific enterprise by reducing the postdoctoral population which keeps growing despite a lack...McDowell! Gary S. McDowell is currently the Executive Director of Future of Research. He is interested in advocating...

...tips can help:  If there’s a teacher, mentor, or other educator working with your audience, ask! They ...refers to the work put into creating a permanent store of knowledge (a schema).”  In scicomm, I like to.... It’s a lot easier to read a formulaic fiction story that doesn’t require any detailed retention of information...knowledge and expertise your audience has is a major factor in how quickly they can understand and process ...

...credit to. Thankfully, as Addgene's resident blog editor, I could take the needed steps to continue where...alternative reagents have reduced immunogenicity. Effector functions are eliminated, as is antibody recognition...constant region. Nonspecific binding, caused cell receptors for the constant region, is also reduced.  Disadvantages...antibody constant region. They are generated using combinatorial libraries, and high specificity has been reported...allowing for them to be used as intracellular inhibitors.  Aptamers are not proteins at all. These high-affinity...

... some exist as dsRNA. Still others use dsDNA to store their genetic information. All of these viruses ...sword – balancing antiviral benefits with the inflammatory response associated with the introduction of...of duplexed RNA Mammalian cells have multiple receptors in place to sense dsRNA and respond accordingly...threshold and thus do not typically activate an inflammatory response. However, if dsRNA sensors are mutated...its way outside of the mitochondria, the same inflammatory response can activate. These types of cellular...