Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 971 results for: lars;

Showing: 501 - 510 of 971 results

  1. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...outlier, this story had us wondering: is there a larger trend here? Do papers associated with Addgene deposits...deposits are more highly cited, we went to one of the largest bibliographic databases available, Web of Science...Biotechnology & Applied Microbiology, Biochemistry & Molecular Biology, and Biochemical Research Methods. We ...at the MIT Sloan School of Management. She is particularly interested in exploring how innovative tools...

  2. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...drastically alter your strategy. I realized that similar types of thinking go into developing gene therapy...PLASMID is a game where you need to apply basic molecular biology, cell biology, microbiology, genetic engineering... He is also founder and consultant at FRESCI particularly interested in advanced in vitro models technology...Resource Center Resources on Addgene.org Find molecular biology educational resources Find protocol videos...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...FPs are tremendously complicated entities with a large range of characteristics (color, brightness, photostability... a project written in Python & Django, using a popular database management system (PostgreSQL), that aims...use case for FPbase is finding information on a particular FP; this can be done directly using the search...your help!), I believe this represents one of the largest available online collections of FP spectra. Every...

  4. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...the cell, and potentially spread out over an even larger area, thus necessitating even higher retrograde...methods to improve AAV production.     References 1.Clark, K. Reed. "Recent advances in recombinant adeno-...Adeno-associated Virus Transduction and Integration. Molecular therapy : the journal of the American Society ...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ...

  5. Hot Plasmids - January 2023

    Type
    Blog Post
    ...levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can be made for expressing...But why might you need a second GFP antibody?  Similarities:  Generated against full length GFP and converted...the group rationally designed GFP variants with similar capabilities. If you’re looking for a super stable...hfYFP retains folding and fluorescence in high molarity GdnHCl, at high temperatures, and in the presence...

  6. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...orchestrated movements of networks of proteins consuming molecular fuel sources. While the importance of understanding... realized that these mutations clustered in a particular region of the R-body. Further mutatgenesis of...penetrate other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond...

  7. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...TCSs have been identified in bacteria, and with similar optimization methods, many of these systems could...

  8. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...contain all fluorescent proteins in common use (particularly not the most recently published ones), they ...if you know that your protein of interest has a similar spectrum. For example, here's a screenshot from...TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission than the mCherry...Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / ...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...with a transfectable cell line. 2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...oligos that consist of direct repeats and crRNA.  Similar to a jigsaw puzzle, the oligos were designed with...

  10. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids...dependent on grants. Aiming to operate under a similar model for our Viral Service, we solicited seed ...support we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...scientists who have gone the extra mile for this particular project. John Doench and David Root from the ...
Showing: 501 - 510 of 971 results