We narrowed to 1,020 results for: NTS;

1. Antibodies 101: Conventional vs Spectral Flow Cytometry

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   Blog Post

   ...cytometry has the ability to generate incredible amounts of data from a single experiment. Although there...

2. Pooled Library Amplifications

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   Blog Post

   ... Genome-wide Inhibition Library. DNA and cell amounts After deciding on a cell type, you will need to...

3. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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   Blog Post

   ... sgRNAs and identified sequence features and adjustments that improved sgRNA activity and thus library...

4. Plasmids 101: Secondary Nanobody Toolbox

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   Blog Post

   ...Nanobodies also simplify multicolor staining experiments because they don’t require using primary antibodies...

5. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   Blog Post

   ... the challenges you face in your career.  Acknowledgements: I would like to thank fellow Addgenies Joanne...

6. Plasmids 101: Colony PCR

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   Blog Post

   ...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

7. Plasmids 101: Protein tags

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   Blog Post

   .... You can get your bacteria to produce massive amounts of protein, but if it’s not folded correctly, there...

8. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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   Blog Post

   ... fluorescent protein to use in your upcoming experiments. In the meantime, if you haven't checked out ...

9. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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   Blog Post

   ...due to both on- and off-target effects, but also presents a strategy to control for these effects. By testing...

10. Hot Plasmids: Fall 2024

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    Blog Post

    ...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...

11. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows for the ...

12. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post

    ...appreciate the opportunity to share these invaluable reagents with the scientific community. Note: Cary Valley...

13. Viral Vectors 101: Pseudotyping

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    Blog Post

    ...King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...

14. Plasmids 101: E. coli Strains for Protein Expression

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    Blog Post

    ...Although the DE3/T7 RNAP system works well for most experiments, the lac promoter can “leak,” meaning that a ...

15. Five Popular Model Organisms

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    Blog Post

    ...hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly to other...

16. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post

    ...biosensors NIR reporters can tell us about cellular events via  fluorescence changes occurring due to protein...

17. Antibodies 101: Beyond Surface Labeling

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    Blog Post

    ...surface of the CD8+ T cells and gate on the positive events for your flow analysis. However, certain downstream...

18. Plasmids 101: Common Lab E. coli Strains

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    Blog Post

    ...strains, especially mutations that make major improvements such as those that increase plasmid yield and...

19. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    Blog Post

    ... cell types. Figure 1: Diagram of AAV components. Created with BioRender.com. AAV vectors ...

20. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    ...directed differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors...