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Capturing a Moment with Marc Zimmer’s “The State of Science”

Type

Blog Post

... the challenges you face in your career. Acknowledgements: I would like to thank fellow Addgenies Joanne...
Plasmids 101: Colony PCR

Type

Blog Post

...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...
Plasmids 101: Protein tags

Type

Blog Post

.... You can get your bacteria to produce massive amounts of protein, but if it’s not folded correctly, there...
Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

Type

Blog Post

... fluorescent protein to use in your upcoming experiments. In the meantime, if you haven't checked out ...
CRISPR 101: Targeting RNA with Cas13a (C2c2)

Type

Blog Post

...appreciate the opportunity to share these invaluable reagents with the scientific community. Note: Cary Valley...
Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Type

Blog Post

...due to both on- and off-target effects, but also presents a strategy to control for these effects. By testing...
Viral Vectors 101: Pseudotyping

Type

Blog Post

...King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV...
Hot Plasmids: Fall 2024

Type

Blog Post

...using mCherry for selection of sgRNA plasmid transformants, they avoid M. abscessus’ high rate of spontaneous...
Twenty Years of Sharing: Addgene's Viral Vector Service

Type

Blog Post

...could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows for the ...
Plasmids 101: E. coli Strains for Protein Expression

Type

Blog Post

...Although the DE3/T7 RNAP system works well for most experiments, the lac promoter can “leak,” meaning that a ...
Five Popular Model Organisms

Type

Blog Post

...hermaphrodites making it easy to conduct large experiments over multiple generations. Similarly to other...
Quick Guide to Near-Infrared Fluorescent Proteins

Type

Blog Post

...biosensors NIR reporters can tell us about cellular events via fluorescence changes occurring due to protein...
Antibodies 101: Beyond Surface Labeling

Type

Blog Post

...surface of the CD8+ T cells and gate on the positive events for your flow analysis. However, certain downstream...
Plasmids 101: Common Lab E. coli Strains

Type

Blog Post

...strains, especially mutations that make major improvements such as those that increase plasmid yield and...
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

Type

Blog Post

...directed differentiation.” Specific cell media, supplements, bioactive small molecules, and growth factors...

Showing: 1001 - 1015 of 1015 results

Results per page:

Capturing a Moment with Marc Zimmer’s “The State of Science”

Plasmids 101: Colony PCR

Plasmids 101: Protein tags

Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

CRISPR 101: Targeting RNA with Cas13a (C2c2)

Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

Viral Vectors 101: Pseudotyping

Hot Plasmids: Fall 2024

Twenty Years of Sharing: Addgene's Viral Vector Service

Plasmids 101: E. coli Strains for Protein Expression

Five Popular Model Organisms

Quick Guide to Near-Infrared Fluorescent Proteins

Antibodies 101: Beyond Surface Labeling

Plasmids 101: Common Lab E. coli Strains

Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation

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