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...You may also like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Background...

...oligos. This is an option that can be added when ordering them or can be performed enzymatically later. ...

...additional charge. What primers were used by Addgene during quality control? Addgene lists the primers used...

...your container. Make sure that any spills produced during the protocol are cleaned up. Discard the weighing...

...bath is a piece of laboratory equipment that helps bring your materials to a particular temperature, catalyze...

...Introduction Restriction enzymes are naturally occurring bacterial endonucleases that recognize a large...

...because bacteria are used as the means for both storing and replicating plasmids. Because of this, nearly...

...and prevents the vector from ligating to itself during the ligation process. If the sticky ends on either...

...and recipient plasmids. Because you lose some DNA during the gel purification step, it is important to digest...

... cells) into each well of the 6-well dish. This brings the total volume in each well up to 1.5 mL. Since...

...and recipient plasmid. Because you lose some DNA during the gel purification step, it is important to digest...

...solution to 990 µL complete medium. Instead of transferring 0.125 µL, now transfer 100 times that volume...