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...month, deciding on the best protein for a given application is a challenge. To help keep you abreast of the...

... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

... of the entire plasmid. Please note that NGS verification is more time-intensive than Sanger sequencing...

...need to determine these transduction patterns empirically for their own experiments. To this end, we offer...

... this limitation, one option is to perform a hierarchical assembly, assembling fragments in multiple steps...

...have good antibodies. The results look almost identical. Addgene: What advantages does your system have...

.... Listen to our podcast interview with Harvard Medical School researcher Connie Cepko to learn about her...

...contracts behind closed doors. Fig. 1: The mythical land of negotiations.    You negotiate everyday...

...use the 2A peptides instead of an IRES when stoichiometrically equivalent levels of expression are required...

...-up Setting up colony PCR reactions is nearly identical to preparing a standard PCR reaction: combine ...

...help you do your job better. #8 Thou shalt enthusiastically foster independence Your partner can make ...

...below. The GUS gene (β-glucuronidase) with histochemical GUS-staining is a very useful visual reporter...

...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...

...had been more careful. 3. Tell a story We are biologically programmed to listen and remember better when...

...focus or zoom on a camera lens without having to physically rotate the rings around the lens. It allowed ...

... Protocols DNA Quantification DNA Quantification You may also like... Addgene’s DNA Quantification Protocol. Protocols... to protein (260/280) is generally used as an indicator of the purity of DNA samples. These days, many...the NanoDrop, if two consecutive samples have significantly different concentrations, it is possible that...between samples if they are expected to vary significantly in concentration. DNA dissolved in water is ...

...Kit (one conical is its own Maxiprep). Critical Do not freeze pellets for later purification. Immediately... Protocols CRISPR Library Amplification CRISPR Library Amplification You may also like... Pooled libraries...quantities of library for experimental applications. Repeated amplifications should be avoided as best as possible...origin of replication and antibiotic selection). This recombination, at a low rate, is not typically a problem...Follow this protocol to perform amplification of CRISPR pooled plasmid libraries in Escherichia coli ...refer to our pooled library material pages for amplification protocols that have been developed by the depositor...available. If a pooled library does not yet have an amplification protocol, the following protocol can be used...

...ultracentrifugation. The arrow indicates the 60–40% interface. The vertical black line indicates the location of the... Protocols AAV Purification by Iodixanol Gradient Ultracentrifugation AAV Purification by Iodixanol Gradient...isomolar density gradient medium suitable for virus purification and isolation of cells, organelles, lipoproteins...and use an iodixanol column gradient for AAV purification. Workflow Timeline Day 1: Purify Day 2: Buffer... to collect the solution, or you will lose a significant amount of your virus. When the first fraction..., et al. "Recombinant adeno-associated virus purification using novel methods improves infectious titer...

... Protocols Plasmid Modification by Annealed Oligo Cloning Plasmid Modification by Annealed Oligo Cloning...added when ordering them or can be performed enzymatically later. Experimental Procedure Digest and purify...

...Affinity Purification with Protein A or Protein G Recombinant Antibody Affinity Purification with Protein...: Buffer exchange Equipment Class II, Type A2 Biological Safety Cabinet 4 °C Refrigerator Pipette controller...controller Benchtop centrifuge compatible with 50 mL conical tubes NanoDrop spectrophotometer 37 °C, 5% CO 2...VWR 89130-900 50 mL pipette, VWR 89130-902 50 mL conical tubes, VWR 89039-656 Microcentrifuge tubes, VWR...inhibitors to 1X if this has not already been done. We typically start with about 250 mL of supernatant and add...buffer. re Collect the flow-through in a 50 mL conical tube and dispose after use. Carefully pour the ...closure and loosen cap. Place column in a 50 mL conical collection tube. Centrifuge column at 1000 x g ...