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...in 2004, we were the plasmid repository. In 2016, we added viral vectors, and in 2022 we added ready-to-use...ready-to-use antibodies. Now, we think of ourselves as a repository for DNA-based materials, including our popular... kits and libraries. This DNA-based materials repository contains 139,868 reagents from 5,821 labs in ...international community, we've partnered with distributors in China, Korea, Japan, India, and Brazil.  ... system for scientists using materials in our repository, from our customer and science support to our...

...amazing stories to share. I spend a lot of time telling scientists about Addgene’s repository, and explaining...student, post-doctoral fellow, lab manager, research technician, or Principal Investigator. How-to guides...for genome editing? Know a professor with a great story to tell? Let us know by emailing us at [email protected] on Twitter and Facebook. We’ll track down the story, get the inside scoop, and keep you informed.   ...

...m a father of three, a scientist and science communicator, and I strive to be a volunteer and contribute...scientist. When our elementary school needed a new coordinator for the annual Math & Science Night, I took the...contacting our school district’s science curriculum coordinator with minimal luck, but eventually the 8th grade...for a scientist. I think that establishing this history helped demonstrate that a) I was a real person ...great feedback from teachers and other science communicators.  What can CRISPR do? I spent more time on...which demonstrated to the students that scientific history was very much in the making. One of the exciting... in an exciting, tumultuous time in scientific history. Whatever your reasons, let us know how it goes...

.... (2007) ‘Improved Gateway Binary Vectors: High-Performance Vectors for Creation of Fusion Constructs ... J. (2002) Arabidopsis : a laboratory manual. Cold Spring Harbor Laboratory Press. Available at: https...expression system Choosing an appropriate expression vector is the first step in generating transgenic Arabidopsis...from the Tsuyoshi Nakagawa lab are binary gateway vectors and can be used for promoter swapping and generating... ‘A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology’, Frontiers in Plant...

...significant holidays. Figure 4: Plasmid request trajectories suggest an exponential decay demand model with... scientists or labs placing orders.  Our model factors the likelihood in our Poisson process into two ... multiplies the likelihood by the corresponding factor 𝜙z if t is during event z. The "events" we considered...each region using stochastic gradient descent in Pytorch, which gave us the maximum likelihood estimates...chemistry and chemical biology.       David R. Liu is Director of the Merkin Institute and Vice-Chair of the ...University; and Howard Hughes Medical Institute Investigator. Liu’s research integrates chemistry and evolution...four examples of technologies pioneered in his laboratory. Learn more here. Additional resources on the...

...Rogers is a postdoctoral researcher in Patrick Müller’s group at the FriedrichMiescher Laboratory of the Max... post was contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab of ... response to an experimental treatment can be monitored. This can reveal previously unknown functions ...splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms to block activity and does not work...phiC31. The popular UAS/Gal4 and Cre/Lox gene regulatory systems have also been successfully implemented..."Single-cell reconstruction of developmental trajectories during zebrafish embryogenesis." Science 360.6392...

...proteins (FP) have also been important in mapping, monitoring, and manipulating neuronal circuits. Epitope ... is therefore difficult to use epitope tags to monitor single molecules - something researchers often ...tags, low FP expression may be insufficient for monitoring a weakly expressed POI. Spaghetti Monster - A... CA3 pyramidal neurons which are known to be notoriously difficult to label. It has also been shown that...technologies such as array tomography, super-resolution STORM imaging, and electron microscopy. Sample preparation...standard proteins tags like GFP. Specifically for STORM, the high intensity signal provided by smFPs can...Spaghetti Monster probes at Addgene Browse all AAV vectors Find ready-to-use AAV preps ...

...reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting an electrode and...successful inhibition of hippocampal neurons. Initial monitoring Second, this may sound rudimentary, but before...exciting—and unanticipated—road of discovery. Monitoring behavioral output The appropriate behavioral ...shock that has become associated with a visual or auditory cue. The hippocampus has long been known to be...versatility and standardization of genetic tools in the laboratory mouse, most optogenetics is performed in this...University of Michigan and he is currently a Postdoctoral Fellow in the lab of Mary Jeanne Kreek at the....    References 1. Stuber, Garret D., et al. "Excitatory transmission from the amygdala to nucleus accumbens...

... Bank (DSHB), directed by David R. Soll, is a repository for monoclonal antibodies, distributing over ...antibodies to be used as a powerful reagent in the laboratory.   The DSHB focuses on monoclonal antibodies,...include those directed against human transcription factors such as PAX7, PAX6, AP-2 alpha and ISLET-1. In ...against new cancer biomarkers. We believe that repositories, such as Addgene and the DSHB, play an important... to ensuring affordable access to materials, repositories provide standardized quality-control and technical...to Our Guest Blogger!   David Soll has been the Director of the Developmental Studies Hybridoma Bank (DSHB...

...Mammalian Vectors By Marcy Patrick Will You Be My Mentor? Finding and Asking for Mentoring Support ...through the lens of Addgene’s blog and plasmid repository. Our blog is just over a year old, and it has...more and more scientists offering to share their stories – about their research, their job hunts, and their... Genes By Marcy Patrick Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered! ...