...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous... artificial intelligence," says Michael Levin, Director of the Allen Discovery Center. The future of this...Use of genetically encoded, light-gated ion translocators to control tumorigenesis." Oncotarget 7.15 (...(2018): 134-144. PubMed PMID: 29684787. Oviedo, Néstor J., et al. "Long-range neural and gap junction ...

... all have a PR-T4 vector backbone, which is a modified version of L440. This vector is versatile in that...of interest into the empty PR-T4P vector backbone. The empty vector PLL4, which is similar to PR-T4P, ...experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology...

...with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator.   ...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you...this category also includes promoters, UTRs, terminators, etc. Once selected, individual units can be ...UTRs, genes, tags, etc.) into MoClo-compatible vectors. Level 1: Assembly of multiple Level 0 parts into...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...developed an engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene...N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout...region VIII of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with... by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat dosing. In a ...

...microscopy, by cloning your protein of interest into a vector encoding a fluorescent tag. Once expressed as a...golgi and then transported in vesicles via the secretory pathway to their subcellular or extracellular ...bacterial components. For example, the Rainbow Vectors from the Mariette Barbier Lab can be used to fluorescently...Edition. 3.  CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka...Barbier, Mariette, and F. Heath Damron. "Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling...

...2002). Insect cells as a factory to produce adeno-associated virus type 2 vectors. Human Gene Therapy, 13...mammalian cells, they do not require serum or an incubator to grow. The virus Insect cell systems are used...references More resources on the Addgene blog Viral Vectors 101: Producing Your rAAV AAV Titers: Where Do They...platform to produce recombinant proteins and viral vectors. Frontiers in Bioengineering and Biotechnology,...

...  Is this department/laboratory collaborative with other departments/laboratories? How long is the average...almost as daunting as the program itself. Picking a mentor comes with many challenges, and the flood of interview...more complex. “How do I reach out to potential mentors?” “Where do I start with picking universities?”...by meeting with professors - a/k/a Principle Investigators (PIs) - whose labs does research that aligns...

...is Head of Synthetic Biology at the Sainsbury Laboratory, where she often feels more like an engineer ...the toolkit. We have included a wider range of terminators than are commonly used as we have tested them...system. The paper also gives quantitative data for regulatory elements, which has not been available for plants...easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene: Do you have...start. They might want to use our promoters and terminators, but then they aren’t sure how to convert their...

...you possess because any potential employers, collaborators, or co-workers want to know that you are a well-rounded...situation? Maybe you demonstrated empathy for a collaborator or co-worker and showed that you really understood... for working with lots of students, managing inventories in the lab, or organizing a research project,...Resources Cross team communication  External collaborators For further advice on highlighting your transferable... for non-academic science careers Learn about mentoring Resources on Addgene.org Check out our careers...

...introduce any one of these topics without some editorializing, and he wisely makes no such attempt. He is...discusses have been making the rounds in journal editorials, science social media, and conference workshops...biology also falls within this section. The GFP story has its own Franklin in Doug Prasher, who originally...describes the detection of gravitational waves--a story I was much less familiar with as a biologist--as...12 years, I’m very familiar with the role our repository has played in these fields, and seeing names ...