Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 971 results for: lars;

Showing: 511 - 520 of 971 results

  1. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated viruses...subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV gene therapies...multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons Retrovirus...High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency...

  2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...back” overhangs, they can be annealed, forming a circular product with four nicks easily repairable by the...terminators. Another potential issue is sequence similarity. If fragments in a multicomponent assembly have...its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, unlike...Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid...

  3. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...Elasticsearch to support the different usage patterns the larger team identified. The basic function of a search...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...depositors (see below). Even if the user experience is similar, the way the software searches through our catalog...

  4. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation...develop and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits...biobuilder.org) or by emailing [email protected]. Of particular interest this fall:  The BioBuilderClub BioBuilderClub...for all who wish to participate. All new teams, large or small, receive the BioBuilder textbook, a laboratory...

  5. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...is a member of the curriculum in Genetics and Molecular Biology, the Dept. of Biochemistry and Biophysics...Generated by Canonical Nonhomologous End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

  6. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ... thus contain complex internal cell structures similar to those of plants and animals. Unlike bacteria...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast... Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “high ... the inability of an organism to synthesize a particular organic compound required for its growth. Many...

  7. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...setup we can observe the whole population of one particular protein in neurons or in any other cell type....attended a scientific symposium called Horizons in Molecular Biology. This annual symposium is organized by...them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins...interests, suspected to interact, are added to a cellular sample. Both antibodies have a short antibody ...

  8. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...This system also allows precise modulating of a particular arm of the signaling pathway (i.e. everything...

  9. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 is synthesized...active enzyme. Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy...

  10. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...Determining which features to verify will depend on the particular plasmid you are working with, but, in most cases... the backbone into the insert. If the insert is large, we may only check the 5’ and 3’ ends to verify ...which is critical for the efficient handling of large numbers of samples. When we grow overnight cultures...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your...
Showing: 511 - 520 of 971 results