We narrowed to 1,158 results for: Gene

...struggling to mutate a specific gene or your anxiously investigating gene therapy options for a rare disease...Epub 2011 Apr 14. Pubmed PMID: 21493687 . Targeted gene disruption in somatic zebrafish cells using engineered...best-selling kit in Addgene’s history. Dr. Keith Joung’s lab at Massachusetts General Hospital also recently... TALENs TALEN Guide Addgene's Guide to TALEN Technologies You may also like....Zinc Finger Consortium Originally published in Addgene's December 2011 Newsletter Overview of TALEN Technology...these plasmids, which are available as a kit from Addgene , custom arrays consisting of 12-31 repeats can...recently released a TALEN kit through Addgene. This kit, first described in Nature Biotechnology in August...

.../10.1073/pnas.2018488117 More resources on the Addgene blog Antibodies 101: Multiplex Immunofluorescence...Immunofluorescence Negative Can Be Positive: Open AAV Data with Addgene Antibodies 101: Choosing the Right Isotype...

...Principal Investigators (PIs). We've interviewed Addgene depositors who are at various stages in their careers...journalism, entertainment, politics, K-12, etc.” Addgene's team includes many PhD scientists who have stepped...

... If you start poking around on Addgene’s Fluorescent Protein Guide to In Vivo Imaging, you’ll pretty ... are always looking to improve upon existing genetically-encoded probes by making them brighter, more ...

...besides structure and isotype determination, don’t generally affect antibody applications like ELISAs or Western...DOI:10.1126/sciadv.abl9171 More resources on the Addgene blog Antibodies 101: Introduction to AntibodiesAntibodies...

...which problems can be addressed and new ideas generated. Furthermore, this casual communication reflects...start something new! Additional resources on the Addgene blog Read our blog post about external collaborations...

...controlling the amount of cutting and mitigating noise generated by off target digestion. After MNase digestion...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...

...their work and their interest in science more generally to people outside of academia. Here are my recommendations...programs at both MIT and Boston University. At Addgene we're committed to scientific sharing – both your...

...video depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps; and an essay on...sport-related concussion.   More resources on the Addgene blog Summer SciComm Series: Cognitive LoadSummer...

...those ideas into commercial products and next generation companies.  We launched in the early spring of...Additional Resources Find more career posts on the Addgene blog Read our recent post on Synbio start up Revbio...

...top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as having... related to these genes below. ID Plasmid Description Industry PI Additional Addgene resources ACE2 plasmids...tables below to find popular plasmids expressing genes encoding SARS-CoV-2 proteins, (spike protein, S;...Addgene's COVID-19 collection of molecular tools and resources available for Coronavirus research. Plasmid...Plasmids Other Coronavirus Plasmids Mammalian Targets General Plasmid Tools Pooled Libraries Other Resources ...understanding of COVID-19 and related coronaviruses. Addgene assisted this effort by providing a platform for... ID Plasmid Description Industry PI Additional Addgene resources SARS-CoV-2 Viral Pseudotyping Collection...

...transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin structure/gene expression...Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused to a...CRISPR? Experts cover CRISPR topics on Addgene's blog. Addgene's Viral Service : Request ready-to-use viral...Cas enzymes generate double strand breaks (DSBs). Nick Mutated Cas nickase enzymes generate single-strand...CRISPR plasmids available at Addgene. Search by species and CRISPR function. CRISPR...technologies are continously developing and expanding. Addgene works with leading scientists in the field to ensure...resources or browse plasmids below by function. Addgene's CRISPR collections feature plasmids for a variety...

... that while this post is intended to provide a general overview of CRISPR components, new Cas9 variants...Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E variant...

...score (Mattei et.al., 2022).    Figure 1: Generation of immunogenicity scores by predictive algorithms...and current Scientist, DNA Sequencing and QC at Addgene.  References and resources References Mosch, ...

...TagRFP to give a brighter signal than mCherry. In general, filter sets designed for Rhodamine / Cy3 will ...set of proteins the green and red proteins are generally the most detectable and so should be used to tag...

...studying up before you begin the hiring process. Read Genevive Bjorn's "Ready, Set, Hire" on Nature Jobs for ...Gilbert: “Very interdisciplinary, with emphasis on genetic engineering, sequencing, and translation of results...

...helpful changes are endless.    Fig. 2: Blugene moves reagents from left to right as they use them...documents/Western%20Blotting.pdf  More resources on Addgene’s blog A Guide to Getting Started in Undergrad ...

...stellar resume and cover letter can be found on the Addgene blog.   3. Personal statement brilliance In addition...co-founder of STEAMid.     Additional resources on the Addgene blog Check out other tools and resources for early...

...This is the fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot... first meeting and get organized. Discuss your general areas of interest and what you want to cover during...

...Framework Learning to think like an immunologist, a geneticist, a physicist, etc., is an immense task (it takes...accessible to your audience.  More resources on the Addgene blog Intro to our Summer SciComm Series...