We narrowed to 1,045 results for: STI

1. Antibodies 101: Secondary Antibodies

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   ...themselves and others that most perplexing of questions: “who moved my goat anti-rabbit?!”*  *a secondary...

2. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   ...and its role in gene expression, though this may still unexpectedly affect nearby genes (Figure 1A-C). ...

3. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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   ...experiments that requires modest gene activation. Synergistic Activation Mediator (SAM) Description SAM uses...

4. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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   ... RNPs, AcrIIA4 reduced off-target editing while still allowing on-target editing (Shin et al., 2017). ...

5. Overcoming the Challenges of Lentiviral Production

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   ...area of the larger vessel to the 10 cm2 dish and adjusting the cell number, plasmid concentration, and experimental...

6. Lab Automation at Addgene

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   ... of pushing them in one by one. It seems very simplistic and it isn’t electronic but it is one of the ...

7. Plasmids 101: Terminators and PolyA signals

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   ...Multiple copies of the Mason–Pfizer monkey virus constitutive RNA transport element lead to enhanced HIV-1...

8. Degrading DNA with Cascade-Cas3

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   ...these systems, though consistent expression can still be a challenge. A multitasking Cas Most Cas effectors...

9. Production of Virus in Insect Versus Mammalian Cells

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   ... start producing virus within 24 to 48 hours. Harvesting timepoints will depend on your specific virus...

10. Antibodies 101: Flow Compensation

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    ...your experiment. So, as long as you are able to distinguish your populations from each other on your flow...

11. Plasmids 101: Protein Expression

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    ...when a protein is expressed through the use of constitutive and inducible promoters. Inducible promoters...

12. Fluorescent Proteins 101: Fluorescent Protein Timers

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    ...differentiation) in mouse embryos (Miyatsuka et al.) Distinguish between models for trafficking to the lysosome...

13. Plasmids 101: Degron Tags

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    ...facilitates ubiquitination of the Halo-tagged protein in question, it is targeted to the proteasome for degradation...

14. Light Sheet Fluorescence Microscopy

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    ...such as in the Ultramicroscope.  In addition, adjusting the thickness of the laser sheet and using multi-view...

15. Transferable Skills Guide: Identifying Your Transferable Skills

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    ... and plan for the future of the team. All while still paying bills and having a life outside my job. The...

16. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    ...plasmid-containing bacteria. So… why do some people still use amp? Carb is expensive (relative to amp), and...

17. Tips from the Repository Trenches: Using Barcodes to Track Samples

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    ...labs. Careful tracking prevents us Addgenies from wasting hours of valuable time searching for samples and...

18. Antibodies 101: Immunoprecipitation

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    ...sample.  Eluting the target protein for downstream testing Once the non-specific proteins are removed through...

19. How to Write a Scientific Review Article

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    ...continuity. Also don’t worry if you feel like you don’t still know enough about the topic. Just get as many words...

20. Plasmids 101: E. coli Strains for Protein Expression

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    ...Arabinose-inducible expression of T7 RNAP; IPTG may still be required for expression F- ompT lon hsdSB (rB...