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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 938 results for: Kin

Showing: 521 - 530 of 938 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ... no fear - plasmids from Addgene depositors are making it easier than ever to use CRISPR  in E. coli and...gene body can repress transcription by physically blocking the elongation complex from binding the DNA or...Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters... Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (2013) Repurposing CRISPR as an RNA-Guided...

  2. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    ...mapping These tools are for viewing, editing or making plasmid maps, but can also analyze and annotate... Viewer: The free SnapGene Viewer is great for looking at plasmid maps and viewing sequencing traces, ... blank spaces, from the text of a DNA sequence, making it ready to copy and paste into other applications...

  3. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    ...video. Don't worry - no pipettes were harmed in the making of this photo!  Here, I was also given a mixture...such as stuffing new depositor welcome kits, note taking for protocol video production, and keeping inventory... my time here comes to an end, my worries about making connections with coworkers seem, in retrospect,...

  4. Fostering communication in remote collaborations

    Type
    Blog Post
    ...Especially when your collaboration includes scientists working in different time zones, it’s important that everyone...accountable to these expectations once they’re set by checking in about them at your recurring meetings and reaching...common. I hope these tips are useful whether you’re looking to improve communication within an existing collaboration...

  5. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    ... cells containing the plasmid construct you’re seeking. Fortunately, there are many ways to do this involving... a successful uptake of your DNA of interest by taking advantage of bacterial characteristics such as ...for specific amino acids. When plated on media lacking the essential amino acid, the bacteria will only...

  6. Sensing Neuronal Dopamine

    Type
    Blog Post
    ...also involved in reinforcement learning, decision making, and motor control.  What do we understand about... 100 nm) of DA. In addition, they exhibit fast kinetics (10 ms on and 100 ms off), enabling detection ...magnitudes of fluorescent responses. Studies on the kinetics of these sensors showed rapid response (60 or ...

  7. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    ...background noise. This is not terribly problematic when working with cell culture, because it is easy to harvest...harvest large quantities of cells. However, when working on specific tissues or organs in whole animal models...major technical hurdle.   ChIP-seq requires  cross-linking during an initial fixation step. This serves to...

  8. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...bunch. They don’t really know where I came from.” Working at eScience Labs, an educational company that creates...that provides scientific editing services. “I was working during the day and editing at night. It was not...moved out here, I was still editing from home and looking for a job.” But Caitlin’s persistence paid off:...

  9. SunTag and Fluorescent Imaging

    Type
    Blog Post
    ...fluorescent signal and enables single molecule tracking  within living cells without affecting protein...managed to track run lengths of the motor protein kinesin across microtubules. Tanenbaum et al. then tested...photobleaching Simplified, long-term single-molecule tracking Caveats of SunTag Very large size: a 24x scaffold...

  10. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...headquarters. I decided to go ahead and apply; thinking I probably would not get selected for both the...compose and submit a manuscript that they were working on currently. Upon arrival to the headquarters,... The most beneficial aspect of this course was breaking up into focus groups with a facilitator. In the...
Showing: 521 - 530 of 938 results