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We narrowed to 971 results for: lars;

Showing: 521 - 530 of 971 results

  1. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...forward and reverse primers). The abundance of a particular barcode sequence then corresponds to the abundance...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...

  2. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    ...of fragments, or the ligation of very small/very large fragments, these problems can be overcome by screening...method, but can be assembled with Golden Gate. The popular Gateway cloning system produces constructs with...leveraged the power of Golden Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo...101: Multiplex Expression of gRNAs Choosing a Molecular Cloning Technique  ...

  3. How to Prepare for an Industry Interview

    Type
    Blog Post
    ... your interview The STAR interview method is a popular way to answer behavioral interview questions. STAR...key decision. Even deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each...

  4. Engaging with science and society at pgEd

    Type
    Blog Post
    ...and how you can set yourself on a path towards a similar position. How pgEd broadens the conversation about...bench scientist completing my graduate studies in molecular biology and epigenetics. So, I sought out a number...be understood by those who are not experts in particular scientific fields. As scientists, many of us ...Find all of our science communication posts Extracurricular activities for a strong science career path...

  5. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells...increasing cloning capacity to ~12 Kb. The ever popular pAdEasy system belongs to this category. 3rd Gen...plasmid (~1 week): The AdEasyTM system is the most popular method for creating adenoviral vector constructs...ultracentrifugation to separate rAdV from other cellular debris. Purified high-titer rAdV is then dialyzed...

  6. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...and resources page Browse our molecular biology protocols Read our molecular biology reference ...scientists to publish findings that don't fit into larger publications and would otherwise be lost in lab...worm so check back frequently for what’s new and popular! References Hammarlund, Marc, et al. "The CeNGEN...

  7. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein antigen...Boguszewska et al., 2019.   Competitive ELISA Similar to a sandwich ELISA, the competitive ELISA is also... visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference...

  8. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...skills Take the DIY approach and start a blog. Stellar talents like Ed Yong jumpstarted their science ..., Scientific American or National Geographic. Similarly, media fellowships from the British Science Association...provides a peak into their day-to-day business with popular work experiences of 3-10 days. And the Society ... Media Production at Imperial College London. Similarly, the University of Cardiff offers an MSc in Science...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    ...need to design and optimize a pegRNA for your particular edit. These tips are a great starting point: ...also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22...

  10. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... than GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, ...Viruses have become a regular part of basic biological research as well as clinical therapy. These biological...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...
Showing: 521 - 530 of 971 results