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We narrowed to 971 results for: lars;

Showing: 531 - 540 of 971 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...edit due to toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei...mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to the original...Ando, R., et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature...with neuron bias observed in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly...

  2. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...avenues for therapeutics that treat disease at the molecular level. For instance, if a disease is caused by...vectors derived from AAV have recently gained popularity as they are uniquely suited gene delivery vehicles...hemophilia B, LPL deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable...on gene therapy for viral infections. She is particularly passionate about HIV cure research and science...

  3. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ... several reasons. Casɸ is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is...cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

  4. Transferable Skills: Negotiation

    Type
    Blog Post
    ... applied for jobs, I knew I should negotiate my salary after receiving an offer but even as I did (successfully... wearing business suits discussing multimillion dollar contracts behind closed doors. Fig. 1: The...prioritize in a lab with both electrophysiologists and molecular biologists? What is their perception of me and...working with you will benefit them too. Ed's note: Salary negotiations will be covered separately in a future...

  5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...isolated, and disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers...existing bacterial CRISPRi methods. First, its modular design allows for customization with strain-specific...generations. Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with...be used in non-model bacteria species where its modular nature allows for the customization of guides, ...

  6. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...believe this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our...Their in Vivo Persistence after Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org...Recombinant Adeno-Associated Viruses. In: Methods in Molecular Biology. Humana Press, pp 247–278 Additional resources...

  7. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... close to the hood so you can view your cells regularly (both before entering the hood and when putting...identified. Even if some mammalian cells lines have similar morphologies, being familiar with these morphologies... the secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need...Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You...

  8. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... generate rAAVs in your own lab with standard molecular biology tools and tissue culture experience. Here...Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular Biology. https://doi.org/10.1101/2023.03.20.533580...cost-effective manipulation of gene expression in the CNS. Molecular Neurodegeneration, 15(1), Article 1. https://doi.org...B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R., Conlon, T., Darmon, C., Doria, M., Douar...

  9. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...the use of degron tags has become increasingly popular for the modulation of endogenously and exogenously...uses a small molecule, a tag, and the endogenous cellular degradation machinery to function. The dTAG is...light up a tagged protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting...proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave off of the protein...

  10. Of Myc and Men

    Type
    Blog Post
    ...long as the disciplines have existed, cell and molecular biologists have been continuously pursuing new...Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still quite small... become one of the most commonly used tags in molecular biology. And the Myc1-9E10 antibody remains a ...experiments. It turns out that even with the most popular antibodies, context is key.    References and...
Showing: 531 - 540 of 971 results