We narrowed to 997 results for: lars;

...orchestrated movements of networks of proteins consuming molecular fuel sources. While the importance of understanding... realized that these mutations clustered in a particular region of the R-body. Further mutatgenesis of...penetrate other barriers (like vasculature or extracellular matrices) in response to pH changes. Beyond...

...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in optimizing...required per system. Applications of these systems The large dynamic range of these systems ensures that they...TCSs have been identified in bacteria, and with similar optimization methods, many of these systems could...

...contain all fluorescent proteins in common use (particularly not the most recently published ones), they ...if you know that your protein of interest has a similar spectrum. For example, here's a screenshot from...TagRFP excitation and the emission filter collects a larger fraction of the TagRFP emission than the mCherry...Fluorescein / Rhodamine / Cy5 (e.g. Semrock's) and the similar 4-laser combination on a confocal (405 / 488 / ...

...with a transfectable cell line. 2) They require larger expression vectors which are often more difficult...into viral vectors. Cas9 multiplexing vectors are larger because they require regulatory sequences to allow...individual promoters).  spCas9 and its gRNAs are also larger than their Cpf1 counterparts. Table 2. Cas9 Multiplexing...oligos that consist of direct repeats and crRNA.  Similar to a jigsaw puzzle, the oligos were designed with...

...preps. This new Viral Service represents Addgene’s largest new initiative since we started distributing plasmids...dependent on grants. Aiming to operate under a similar model for our Viral Service, we solicited seed ...support we are able to provide faster access to a larger number of viral preps. Visit our Sponsors and Supporters...scientists who have gone the extra mile for this particular project. John Doench and David Root from the ...

...in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated viruses...subject delivery, as immunogenicity was previously a large barrier for gene therapy. In fact, AAV gene therapies...multiple diseases, including hemophilia B and spinal muscular atrophy.   Type of Virus Pros Cons Retrovirus...High Gene Expression Infects Non-Dividing Cells Large Packaging Capacity (>8kb) High Transduction Efficiency...

.../phototoxicity Not susceptible Susceptible Subcellular imaging Improving Well-established High-throughput...bioluminescent reporter to be cloned and remains popular, other luciferases have been identified in the ...luciferase is measured via the light produced. A similar approach can be used to analyze cell signaling ...brings the two luciferase components together. A similar approach can test protein-protein interactions ...

...back” overhangs, they can be annealed, forming a circular product with four nicks easily repairable by the...terminators. Another potential issue is sequence similarity. If fragments in a multicomponent assembly have...its low cost makes it ideal for researchers doing large amounts of cloning. Assembly is scarless, unlike...Protocol Gibson Assembly Blog Post Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid...

...Elasticsearch to support the different usage patterns the larger team identified. The basic function of a search...scientific domain in general and of our data in particular and our deep understanding of Addgene’s customer...categorization of our information in order to more granularly customize the search engine, and a scoping mechanism...depositors (see below). Even if the user experience is similar, the way the software searches through our catalog...

...of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation...develop and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits...biobuilder.org) or by emailing [email protected]. Of particular interest this fall:  The BioBuilderClub BioBuilderClub...for all who wish to participate. All new teams, large or small, receive the BioBuilder textbook, a laboratory...