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We narrowed to 938 results for: Kin

Showing: 541 - 550 of 938 results

  1. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... Why and Where Do I Find One? If you need help making a transition into a new job or maybe to an entirely...gene delivery, but have limited packaging capacity making them difficult to use with CRISPR as the cargo..... 10. Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your...

  2. Hot Plasmids - January 2023

    Type
    Blog Post
    ...hot plasmids articles. Think we missed something smokin'? You can pitch a Hot Plasmids section (antibodies...heatshock promoter) drives expression of the tumor-seeking CAR (chimeric antigen receptor), whereas a ubiquitous... variants with similar capabilities. If you’re looking for a super stable FP for your next microscopy ...

  4. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ... apart sources of information they don’t like, looking for reasons to dismiss them. There’s evidence to...Zealand’s multiple Covid-19 lockdowns, I held mask-making workshops in my community. I brought along printed... to better meet the needs of the people we are talking to. Emma Harcourt is a PhD student in the Department...

  5. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...Check out this post for a refresher on AAV titers Making contact with a cell Although an individual AAV ... this type of vector to such a high titer when looking for effective gene expression. How do you achieve... Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production.     References...

  6. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...“reading”) of DNA, have changed our collective thinking about the feasible size and scope of projects ...library prep. As part of our mission, we’ve been working with Addgene to develop and apply our plexWell™...read level, and because custom primers or "primer-walking" reactions are completely unnecessary for sequencing...

  7. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...biological motion (whether endocytosis, vesicle trafficking, or muscle contractions) are produced by orchestrated...the ciliate. It’s thought that they function as a kind of armor to protect their host from predation (Petroni...

  8. Components of CRISPR/Cas9

    Type
    Blog Post
    ...Cas9, and the tracrRNA which acts as a scaffold linking the crRNA to Cas9 and facilitates processing of...different components of CRISPR, It's time to start thinking about how you can used them in your experiments...experiments. Use the following flow chart to get you thinking about the types of experiments you're planning on...

  9. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...idea to take some time to confirm that you are working with the correct construct, and verify that the...Consult the manufacturer’s manual for the optimal working conditions for each enzyme. Watch out for methylation...run control uncut DNA to ensure your enzymes are working. When uncut plasmid DNA is isolated and run on ...

  10. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    ...think. So, if you’ve ever wondered what goes into ‘making virus’ or what gag and pol stand for, we demystify...recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This is typically supplied...discussed are compiled for reference below. Happy virus making!  References and Resources Kulanayake, S., Tikoo...
Showing: 541 - 550 of 938 results