...ground and what the founders hope to see in the repository’s future. If you’ve ever been curious about what...be successful and the importance of lentiviral vectors in ensuring that success Chapter 6 - Developing...distribution!) Chapter 8 - Opportunities to create repositories with other materials Chapter 9 - The Founders...computer systems and networks. Benjie was Addgene's Director of Technology for 7 years, and is now Chief Software...

...Joanne Kamens, Addgene’s executive director, said during our celebratory lunch, it’s hard to picture one ... the scientists who deposited plasmids to the repository and for the scientific sharing community (check... in 2016 and we just reached our 10,000th viral vector shipped! In the upcoming months, we will be moving...

...? 11:51 - 13:28 : How do you define what a good mentor is? 13:29 - 14:31 : What drives you to do science...the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve... mentorship... mentor...

...including vector-insert joining for recombinant plasmid construction (restriction cloning), adaptor ligation...reliably detect single-base differences. The original creators of this technique visualized the ligation product...specific base sequences, but are sensitive to distortions in helix shape (22). Large purine:purine mismatches... the Thermostable Ligase Reaction Temperature Calculator can be used to estimate an appropriate incubation...

...Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d ...kit creation and quality control efforts with depositors; Gizela, the lab member who knows how the physical...responsible for guiding new deposits into the repository, performing quality control, and providing technical...Jefferson dined alone.” There was a time in human history when an intelligent, motivated person really could...it alone. The help of fellow lab members and collaborators will not only get your paper out the door much...

...your community, think hard about picking a good mentor, and begin considering career options early. Tune...Depace's Talk on "Precision and Plasticity in Mentoring" We'd like to thank Niroshi and Ben for taking...the Addgene Blog Get Advice on Choosing a Good Mentor Learn How the Future of Research Looks to Improve... mentor...

... the party going! At Addgene, we’ve set-up an exhibitor booth in order to help scientists find the plasmids...benefit from depositing their constructs to the repository, and getting feedback from our community. It ...Zhang was awarded one of this year’s SfN Young Investigator Awards and we couldn’t wait to congratulate ... to help their labs save time and resources by storing and distributing their plasmids with the scientific...

...editing (SECURE) base editors Keith Joung’s lab recently found that a cytosine base editor (CBE) with rat APOBEC1... tethering of DNA template to Cas9 SECURE base editors Nme2Cas9 CRISPR interference in Candida albicans...Read the paper in Nature Find the SECURE base editors All-in-one AAV delivery of Nme2Cas9 with gRNA ...

...resources. In 2022 alone, our website saw 2.2 million visitors from 231 countries. Guides and protocols were ...beloved blog received 1.3 million page views from visitors in over 200 countries. We, like everyone around...have many successes to reflect upon, a wealth of history to learn from, and a compelling future to plan ...forward to working with Addgene colleagues, collaborators, partners, and the larger scientific community...

...published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates. Scientists must do science ...in the Netherlands. There are many academic administrators who are finding ways to provide new types of...designing career exploration programs or peer mentoring groups. But for now, you can click here to register...October 2-3, 2014 Boston University Jacob Sleeper Auditorium, Boston, MA Register on Eventbrite (This is a...