We narrowed to 997 results for: lars;

...deletions) if NHEJ joins the distal ends together. Similarly, it may be possible to direct insertion of an ...is a member of the curriculum in Genetics and Molecular Biology, the Dept. of Biochemistry and Biophysics...Generated by Canonical Nonhomologous End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel... CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens        ...

... thus contain complex internal cell structures similar to those of plants and animals. Unlike bacteria...plasmid copy number within the bacterial cell. Similarly, the specific ORI elements included within a yeast... Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “high ... the inability of an organism to synthesize a particular organic compound required for its growth. Many...

...setup we can observe the whole population of one particular protein in neurons or in any other cell type....attended a scientific symposium called Horizons in Molecular Biology. This annual symposium is organized by...them at the MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins...interests, suspected to interact, are added to a cellular sample. Both antibodies have a short antibody ...

...threonine kinase with a similar 3D structure to MEK1, but Raf1 and CDK5 have less similarity. For these three...versions of MEK1, MEK2, Raf1, and CDK5 all behave similarly to their endogenous counterparts. Additionally...This system also allows precise modulating of a particular arm of the signaling pathway (i.e. everything...

...phosphatase activity: In yeast, the PHO8 gene encodes a vacuolar alkaline phosphatase. Normally PHO8 is synthesized...active enzyme. Pho8 phosphatase activity or the molecular weight shift of Pho8 from its uncleaved to cleaved...than tracking generic autophagy markers. It’s particularly useful for comparing bulk autophagy vs. mitophagy...Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy...

...Determining which features to verify will depend on the particular plasmid you are working with, but, in most cases... the backbone into the insert. If the insert is large, we may only check the 5’ and 3’ ends to verify ...which is critical for the efficient handling of large numbers of samples. When we grow overnight cultures...submission instructions. As for the analysis of a large number of sequencing reactions, you can make your...

...actually really research oriented [and] somewhat similar to an academic research environment” with a strong...forward and reverse primers). The abundance of a particular barcode sequence then corresponds to the abundance...multiple replicate populations, they found that similar barcodes consistently made up the majority of the...Vivo Pharmacology at Novartis Oncology and is particularly interested in applying what we have learned ...

...of fragments, or the ligation of very small/very large fragments, these problems can be overcome by screening...method, but can be assembled with Golden Gate. The popular Gateway cloning system produces constructs with...leveraged the power of Golden Gate cloning into a modular cloning strategy. Sometimes referred to as MoClo...101: Multiplex Expression of gRNAs Choosing a Molecular Cloning Technique  ...

...cells for mycoplasma (Link opens in a new window) regularly Do not over or under-grow your cells. Thaw a new...well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor the growth of the cells...selection. It is important to monitor these cells regularly and replace the cell media. Cell death by some...cells in the culture, so it is important to do regular media changes and maintain optimal growth conditions... or removed from the culture, check the cells regularly to confirm transgene expression. As polyclonal... individual wells become confluent, expand into larger vessels. A confluent well of a 6-well dish can ...

...indirect ELISA is very similar to a direct ELISA, aside from the detection step. Similarly, the protein antigen...Boguszewska et al., 2019.   Competitive ELISA Similar to a sandwich ELISA, the competitive ELISA is also... visualization. The indirect ELISA is used for similar experiments as the direct ELISA, the only difference...