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We narrowed to 971 results for: lars;

Showing: 541 - 550 of 971 results

  1. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... of the vocabulary--DNA, protein, plasmid, gene, etc. Though I talked about CRISPR’s molecular mechanism...unethical manner, but I also tried not to be an alarmist.  The overarching guideline for all of these ...thanks to Joanne Marks, Jonathan Marks, and Vanessa Bullard of Chenery Middle School for their tremendous guidance...

  2. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...consisting of dCas9 and targeting gRNA, to identify molecular interactions at this site (Fujita and Fujii, 2013...and RNA sequencing (Fujita et al., 2015). Using similar affinity purification with an anti-Cas9 antibody...inappropriately expressed can cause facioscapulohumeral muscular dystrophy (FSHD), a disease for which there is...29480524. Gilbert, Luke A., et al. "CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes...

  3. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...developed into some of the most widely used tools in molecular biology. “Retroviruses” is an umbrella term which...mutagenesis (which HIV does not do). Furthermore, molecular biologists working with vectors are, in most cases...on addgene.org Addgene's Lentiviral Guide Most popular lentiviral plasmids γ-Retrovirus Guide References...synthesis with 70 S viral RNA as template. Journal of Molecular Biology, 79(1), 163–183. https://doi.org/10.1016...

  4. Great Results Start with Great Standard Curves

    Type
    Blog Post
    .... Standard curves are critical for a number of popular scientific applications, and the quality of a standard...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL). The standard...consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use duplicates or...

  5. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...produce your own recombinant antibodies with some molecular biology and cell culture experience. Let’s go ...further processing. It may be helpful to incorporate regular feeds during this incubation time to maintain cell...cells, and debris. Affinity chromatography is a popular choice for this. Chromatography columns containing...Grimsley G, Gray T. How to measure and predict the molar absorption coefficient of a protein. Protein Sci...

  6. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...and I guarantee you have developed them through similar experiences in graduate school. These skills will...they use, you’ve gained experience working with similar protocols, and look - they’re seeking someone who... be assured that you will contribute openness, clarity, approachability, and professionalism to team meetings...strength until it is identified by another person. Similar reasoning may also apply to other acquired skills...

  7. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ...groups or, by extension, race. Many researchers do large-scale CRISPR screens in cancer cell lines, looking...be good targets for therapeutic development, particularly in cancers that are more common in individuals...address this issue, but it still points to a far larger and more complex problem endemic to biomedical ...confounding factor when interpreting findings, particularly when researching ancestry groups. And, of course...

  8. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...More recently, online journal clubs have become popular, including those based on Twitter. At my former...interest from relevant parties, and the company at large, by polling and informal communications we made ...winding down. Perhaps other projects have taken on a larger priority for the participants. Perhaps people feel...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed...

  9. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...lab bench, we will know that a particular barcode and therefore particular sample is missing so that we ...information in a small space and is ideal for tracking large collections. Many companies, such as Thermo Fisher...preparation, and analysis from our protocols and molecular biology reference pages! Resources Avaliable on...

  10. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ..., mid- to late-career grant professionals have similar stories about a time when their passion for an ...was asked if I would consider consulting for a particular grant by another state. With the blessing of ...professional development. Do you want to work in a particular area, e.g. education, poverty, environment, early... Associates have secured more than $70 million dollars in grant funding. Jo serves on the Board of the...
Showing: 541 - 550 of 971 results