We narrowed to 938 results for: Kin
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TypeBlog Post...think. So, if you’ve ever wondered what goes into ‘making virus’ or what gag and pol stand for, we demystify...recombinant adenovirus is missing the endogenous E1 gene, making it replication deficient. This is typically supplied...discussed are compiled for reference below. Happy virus making! References and Resources Kulanayake, S., Tikoo...
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Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...can begin to understand how the brain works. It’s kind of like “the head bone’s connected to the neck bone... to the rest of the body. Therefore, by simply looking at how neurons are physically connected we can ...efficiently deliver genetic information or tools, making them useful tracers, but finding a virus that works... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post..., you may be curious about all of the different kinds of antibodies that are available. One common type...References Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...TA, Humphries MJ (2016) Ligand-induced Epitope Masking. Journal of Biological Chemistry 291:20993–21007... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...— by directly typing terms in a search bar, by ticking some boxes, by selecting a suggestion — how should...resources. Filters We identified the most useful kinds of information per resource category to expose as...our search indices in order to support different kinds of matches: exact words, partial words, phrases,... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...great? It produces a ~10 fold higher titer virus, making it ideal for CRISPR pooled library screening experiments...V2.0 was made available through Addgene in 2015, making this the most recently available plasmid in the... thank all of our depositors and requestors for making their reagents available and for supporting scientific... -
AAV Q&A with Tim Miles
TypeBlog Post...question that didn’t get answered live - but he kindly took some time to address all your unanswered questions...astrocyte specificity and would like to get it working in microglia as well. A: We are not aware of any... tail vein injections but would like to avoid "leaking" into the brain, would you recommend using the ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post...reference protein) under identical conditions, a ranking of the practical brightness can be made (Goedhart...repeating this with another fluorescent protein, a ranking of practical brightness can be made. This strategy...their practical brightness in a system closely mimicking the future application. In figure 2 we provide... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog Post...high-throughput manner and therefore push us closer to making cancer precision medicine a reality. Challenges...Since time is of the essence in clinical settings, looking ahead, we’re starting to build saturating libraries... Institute of Harvard and MIT and is focused on making precision functional genomics a reality for future... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post... it’s working. You can only do that screen with a system you are really confident about working for most...made standard protocols available and they are working to further optimize those now. They are also developing... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...better fuels, feeds, foods, and fibers. And because working with plants can be challenging, there are a lot...construct our color changing flower we are carefully making our way through the IP landscape, using plant parts...novel pieces where applicable. We will also be working with open source, transformation technologies like...