...the same page with the potential PI in terms of mentoring style and expectations. Make sure you will enjoy... Tsang, Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting...about what's out there.”- Joanne Kamens, Executive Director Career exploration “You can't wait for opportunities...my team at Addgene was created.”- Eric Perkins, Director of Science Product Management “I knew very early...

... Best Way to Elute and Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris...posts on COVID-19 featuring plasmid tools from depositors who quickly shared their reagents: detection ...expression, and more. We also highlighted reagent repositories and other collaborative efforts in the scientific...

...   Alexa Fluor 700APC-H7APC-Cy7   Many factors can influence the level of brightness beyond the...detected through a cell surface label. The viral vector also encodes for eGFP, which serves as a transduction...example, point at issues with the double-expression vector.) In our flow experiment, we'll want to identify.... His projects include investigating negative regulators of T cells and cytokine signaling in tumor immunity... Guide Ready-to-use antibodies in the Addgene repository Antibody Protocols ...

...member of the Addgene Advisory Board, and Associate Director of the Genetic Perturbation Platform at the Broad...per gene. Further, the pools are available in two vector formats: all-in-one (lentiCRISPRv2, expressing ...both Cas9 and an individual gRNA from this single vector) and guide-only (lentiGuide expressing only an ...blogger, John Doench! John Doench is the Associate Director of the Genetic Perturbation Platform at the Broad...

...other researchers, and cloned them into lentiviral vectors to generate a kit which is now available at Addgene...cDNAs into a single consensus lentiviral expression vector and attached a DNA barcode to each to make the ...environments, for example. “We’ve been contacted by investigators all over the world who are interested to use...researchers have plans to expand the library to include activators for more cancer-related pathways and more nodes...

...yeast. These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5...from these yeast-optimized fluorophore tagging vectors for your single or multi-color imaging experiments...protease site)-6xGly linker-(epitope tag)-ADH1 terminator-(selection marker). Additionally, Dr. Formosa...

... created the IBEX Knowledge-Base, a community repository and resource for spatial biology enthusiasts....includes 1049 community-validated reagents from 20 contributors, including 61 fluorescent probes, representing... showing the IBEX Knowledge-Base, with every contributor listed as an author.  The IBEX Knowledge-...Imaging Community’s vision is an open, global repository for reagents, protocols, panels, publications...

...have deposited their plasmids into the Addgene repository. More than 4,500 plasmids have already been deposited...aware of what Addgene can do for them and how the repository can help them share their materials. The new ...macrophages/monocytes using lentiviral expression vectors. I also used plasmids made and shared by others...carrying out my studies, and getting them from collaborators was extremely helpful, but with hindsight I ...

...bacteria, plants, and yeast. Popular cytosine base editor pCMV-BE3 has been requested nearly 400 times, and... plasmids covering three different epigenetic effectors. The diversity of tools is especially exciting... subpages, our updated CRISPR Guide and CRISPR History provide a great overview of the development of ...resources: CRISPR Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

...pre-clinical and human trial phases.   So how does CRISPR factor into this workflow? CRISPR both speeds up the process...mutations and then test both individual and combinatorial drugs on for effectiveness prior to patient ...breast cancer and identified several candidate inhibitors of gene X in a screen. You generate a gene X ...CRISPR Guide CRISPR Plasmids and Resources CRISPR History and Development for Genome Engineering    ...