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We narrowed to 971 results for: lars;

Showing: 551 - 560 of 971 results

  1. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...from the David Liu and Dali Li labs reported a similar tool shortly thereafter, using mouse instead of...the base editor. Black arrows indicate natural cellular processes. Created with BioRender.com. The...mitochondrial base editing to base editors optimized for particular species and more, base editors are getting smaller... programmable T-to-G and T-to-C base editing. Molecular Cell, 84(7), 1257-1270.e6. https://doi.org/10.1016...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...ubiquitin E3 ligase cereblon (CRBN), including popular new approaches like PROTAC targeted protein degraders...useful addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications...luminescence wavelength (460 nm) is shorter than many popular fluorescence markers like GFP (510 nm), allowing...Prakash, M., & Dunn, A. R. (2024). Split Luciferase Molecular Tension Sensors for Bioluminescent Readout of ...

  3. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...and debris. The side scatter roughly measures granularity, a proxy for nucleic complexity of the cell.  ...antibodies conjugated to fluorescent markers and/or cellular dyes and stains. These will be used in a series...they did in their experiments and determine if a similar approach would be good for your work. Hopefully...., Pons, J. A., & Muro, M. (2018). In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates...

  4. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    ...Going Deeper: Biomolecular Tools for Acoustic and Magnetic Imaging and Control of Cellular Function. Biochemistry...difficult. The first-generation mARGs also had a large genetic footprint (two cassettes + a booster cassette...experiments, simply induce injected engineered cells via regular intraperitoneal doxycycline injections to enable...

  5. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ... the symptoms of mesothelioma are usually very similar to the symptoms of other diseases, such as asthma...diagnosis indicate, for Stage 3, that the cancer is large in size and may have spread while Stage 4 indicates...with new ways to discover the cancer earlier, in particular before it starts to spread beyond its point of...tumors. Studies have shown that bevacizumab is particularly effective when used in conjunction with standard...

  6. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...  Physical titer is typically calculated by two popular PCR-based methods: Quantitative PCR (qPCR) Digital...generating a standard curve of known concentration. We regularly make and validate fresh plasmid standards. We...). However, because purified AAV preps contain similar and low levels of background protein and chemical...email us and we can do our best to send you a particular lot (if we still have it available). References...

  7. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...so great. If we all came from similar backgrounds, we would have similar cognitive biases and this would... information. It is quite possible that the clarifications lead to the story being scrapped or broken ...While writing the code, we may need additional clarification and again we collaborate with the Product Team...

  8. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ... interfering with downstream assays. This is particularly advantageous if the antibody used for IP is ... and structure. Their porous surface provides a large area for binding, but antibodies conjugated within...despite the size difference. Magnetic beads are particularly advantageous because they do not require centrifugation...binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used to isolate proteins ...

  9. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...for these faults. However, it’s still unclear if similar pitfalls exist for CRISPR screens and how best ...off-target cutting patterns in genome-wide screens similar to patterns seen in single-guide studies? Many ... Short gRNAs have lower off-target cutting and similar on-targeting cutting as full-length gRNAs Lastly...gold standards for human functional genomics." Molecular systems biology 10.7 (2014): 733. Pubmed PMID:...

  10. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...transcription factors, therefore promoters play a large role in determining where and when your gene of ...between cell types or organisms, promoters must be similarly variable. Bacterial promoters only work in prokaryotic...related species from which they were derived. Similarly, the various eukaryotic cell types (mammalian,...Repressible Gets turned off with high levels of cellular tryptophan. lac General expression Promoter ...
Showing: 551 - 560 of 971 results