We narrowed to 997 results for: lars;

... your interview The STAR interview method is a popular way to answer behavioral interview questions. STAR...key decision. Even deciding to stop pursuing a particular line of research is a deliverable. Find 10 steps...answers rings true for you, go ahead and give a similar answer in a following interview. Expect to be tired...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each...

...can be produced at high titers, can accommodate large transgenes, transduce quiescent and dividing cells...increasing cloning capacity to ~12 Kb. The ever popular pAdEasy system belongs to this category. 3rd Gen...plasmid (~1 week): The AdEasyTM system is the most popular method for creating adenoviral vector constructs...ultracentrifugation to separate rAdV from other cellular debris. Purified high-titer rAdV is then dialyzed...

...edit due to toxicity from electroporation or poor cellular entry with other transfection methods. The Junwei...mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to the original...Ando, R., et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature...with neuron bias observed in young macaques and vascular bias in mature marmosets. CAP-Mac also robustly...

... than GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, ...Viruses have become a regular part of basic biological research as well as clinical therapy. These biological...classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate into the host...

...need to design and optimize a pegRNA for your particular edit. These tips are a great starting point: ...also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation... from degradation. When designing epegRNAs with large structured motifs like mpknot, use the pegRNA Linker...Enhanced prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22...

...and resources page Browse our molecular biology protocols Read our molecular biology reference ...scientists to publish findings that don't fit into larger publications and would otherwise be lost in lab...worm so check back frequently for what’s new and popular! References Hammarlund, Marc, et al. "The CeNGEN...

...and how you can set yourself on a path towards a similar position. How pgEd broadens the conversation about...bench scientist completing my graduate studies in molecular biology and epigenetics. So, I sought out a number...be understood by those who are not experts in particular scientific fields. As scientists, many of us ...Find all of our science communication posts Extracurricular activities for a strong science career path...

... several reasons. Casɸ is only ~70kDa, with a molecular weight roughly half of Cas9 or Cas12a. This is...cells with unique spatiotemporal phenotypes from large heterogeneous cultures.  HeLa cells expressed...without any reduction in performance compared to larger genome-wide libraries. Find the library here ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

...avenues for therapeutics that treat disease at the molecular level. For instance, if a disease is caused by...vectors derived from AAV have recently gained popularity as they are uniquely suited gene delivery vehicles...hemophilia B, LPL deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable...on gene therapy for viral infections. She is particularly passionate about HIV cure research and science...

...skills Take the DIY approach and start a blog. Stellar talents like Ed Yong jumpstarted their science ..., Scientific American or National Geographic. Similarly, media fellowships from the British Science Association...provides a peak into their day-to-day business with popular work experiences of 3-10 days. And the Society ... Media Production at Imperial College London. Similarly, the University of Cardiff offers an MSc in Science...