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...mentors all around you. This third article in the Addgene Blog Mentoring series will cover 2 of the 7 mentoring...

...didn’t need to be strictly followed in order to generate high-fidelity reactions. (Need a refresher? The...

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...Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has ...out our Suggest a Plasmid form or e-mail help@addgene.org to help us improve this resource!...

...Adeno-associated virus (AAV) vectors are safe and useful gene delivery tools that effectively transduce many mammalian...Packaging Service overview. Function Chemogenetics DREADDs, PSAMs Optogenetics Channelrhodopsins, Halorhodopsins...Ready-to-use adeno-associated virus (AAV) available from Addgene's viral vectory packaging service. Browse constructs...preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality control process...shipment. If you can't find the prep you need, try Addgene's AAV Packaged on Request service. You can choose...

...markers of general cellular organelles and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent...cytoskeletal elements. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 26722 GFP-cortactin...cellular junctions. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 71367 Human Beta-catenin...pathways and structures. ID Plasmid Structure Targeting Gene/Signal Fluorescent Protein PI 11546 EGFP-LC3 Autophagosomes...Fluorescent Proteins: Subcellular Localization General Cytoskeleton Junctions Membrane Trafficking Additional...cytoskeleton remodeling or blocked membrane traffic. Addgene has assembled a collection of popular fluorescent...your protein is targeted to the same structure. General Structures Eukaryotic cells contain a variety of...

... of Gateway-compatible pDEST vectors. ID Plasmid Gene/Insert Industry PI Pooled Libraries CRISPR and overexpression...engineering forms the core of the deorphanizing engine. Genetic and proteomic interactions represent the framework.... Protein production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded...integrated knowledgebase that features, among others, genetic variants and disease associations. Plasmids This...overexpression pooled libraries are useful tools for genetic screening experiments. These libraries target the...Plasmid Collection NeuroMab Plasmid Collection Addgene Blog: Gateway Cloning Content last reviewed: 12...

...an empty backbone can be repeated to ligate your gene of interest after the promoter, and so on. The following...

...-mouse" or "anti-rabbit") ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions ...Find information on Addgene's collection of plasmid-based antibodies, including monoclonal antibodies...Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant antibodies...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More...animals or hybridoma cells, but are difficult to generate due to expense and time. An alternative approach...fragment libraries from chickens Methods for the generation of chicken monoclonal antibody fragments by phage... 2015 Oct;167(2):95-102. Joanna Bereta Sybody Generation Toolbox for making synthetic nanobodies (sybodies...

...currently available to industry scientists. ID Plasmid Gene/Insert PI Kits Kit name Type PI Description High...Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search Use Addgene's Search to...protection for material providers. Addgene's deposit agreements allow Addgene to distribute plasmids on behalf...can be submitted to Addgene through your user account or by email to mta@addgene.org . After we review ...A portion of Addgene's collection is available to qualified for-profit entities. Addgene reserves the ...Addgene has a growing collection of plasmids that are available to industry. Learn more about the available...assembling standardized parts to create eukaryotic multigene constructs. ONE-GO Biosensors Kit Fluorescent ...

...site-specific recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page for more...236288 pAAV.TBG.PI.FLPo.rBG TBG none 8 Melina Fan , Addgene Research Program Light-Inducible Recombinases ID...

...opsins , genetically-encoded calcium indicators, and rabies targeting genes. Recombinases Addgene ID Plasmid...definitions (e.g. neurons defined by a single gene or projection). Intersectional expression approaches ... in well-defined cellular sub-populations has generally been limited to single-component cellular definitions...cells based on a doubly-specified combination of genetic and/or anatomical-defined parameters, by placing...Operating Procedure (Link opens in a new window) with general principles for working with INTRSECT recombinases...pAAV-CaMKIIa-Flpo None Yes Single recombinase-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...Vcre No Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral...

... disorders by enabling region- and cell-specific gene-delivery in the mouse brain through these human ...List of Pleiades MiniPromoters MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A pEMS1142 EGFP/NLS...

...fluorescent biosensors to measure biomolecules or genes via FRET or other assays....specific biomolecules, the activity of specific genes and cellular processes, or other factors like environmental...mitochondrial oxidative stress A novel MitoTimer reporter gene for mitochondrial content, structure, stress and...for a sensor for a different analyte, a sensor for gene-specific activity, or for popular biosensors in ...mTORC1", "mitochondria"). ID Plasmid Description Gene/Insert Vector Type Eligible for Viral Packaging ...Gadella Calcium Teal genetically encoded calcium sensor T-GECI Blue-shifted genetically encoded Ca 2+ indicator...WHaloCaMP1 chemigenetic calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium...

...associated with Addgene materials. Plasmids ID Plasmid Description PI Viral Preps Addgene distributes ready-to-use...these antibodies are available at Addgene. AAV Viral Service : Addgene provides a viral service to help...the revolutionary dynamic picture of the brain generated through this effort will provide novel ways to...enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter Edward ...enables single cell, single millisecond resolution optogenetics. Cre-dependent virus Edward Boyden 108422-AAV5...Hillel Adesnik 111066-AAV1 pAAV-hSyn-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...promoter Lin Tian 111067-AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1...

...helper plasmid (Addgene #81070) . This serotype permits robust retrograde access and transgene delivery to...Cre-dependent Optogenetics Karl Deisseroth 26973 pAAV-hSyn-hChR2(H134R)-EYFP Syn Activator Optogenetics Karl Deisseroth...Flp-dependent Optogenetics Karl Deisseroth 58880 pAAV-Syn-ChR2(H134R)-GFP Syn Activator Optogenetics Edward ...Activator Optogenetics Edward Boyden 59171 pAAV-Syn-ChrimsonR-tdT Syn Activator Optogenetics Edward Boyden...Activator Optogenetics Gordon Fishell 65014 pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Inhibitor Optogenetics Edward ...Cre-dependent Optogenetics Edward Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CamKII Inhibitor Optogenetics Edward...Inhibitor Optogenetics Ofer Yizhar 154948 CaMKII-somBiPOLES-mCerulean CamKII Bidirectional Optogenetics Simon...

...distributed by Addgene have been produced in-house by Addgene scientists. They are generated using standard...or by contacting help@addgene.org . Some of the antibodies distributed by Addgene were developed by the...Overview of Addgene's antibody production methods and quality control processes for purified recombinant...optimized for each specific antibody in order to generate high quality preparations. Following production...titered and undergo stringent quality control by Addgene or a trusted quality control partner before distribution... preparations developed at IPI may differ from Addgene antibodies in “Production & Usage” and “Delivery...using ImageJ software and a standard curve is generated. The final antibody concentration is calculated...

...Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate. Image...Ready-to-use AAV available from Addgene's viral service. Engineered systemic constructs from Viviana ...As part of our Viral Vector Packaging Service , Addgene is distributing ready-to-use viral preparations...depth descriptions of each serotype, check out Addgene's blog on Systemic Capsids . To review performance...data of each of these serotypes, please refer to Addgene’s Data Hub and the Caltech CLOVER Center’s repository...produced with the pUCmini-iCAP-PHP.eB plasmid (Addgene #103005) . Note on PHP.eB tropism and specific ... BiSSTe4 dTomato/nlsdTomato Control Fishell Chemogenetics 44361 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Syn Activator...

...vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from Addgene and scientists... production at Addgene, ordering information, and more. Additional Resources Addgene's Data Hub Curated...Overview of Addgene's viral preparation service for plasmids and pooled libraries. Viral preparation ... Viral Vector Packaging Service Addgene offers flexible packaging options so you can easily...viral vector is produced from a cargo plasmid in Addgene’s collection. These plasmids are created by your... to do when you receive your viral vector from Addgene, with details about storage. Biosafety Guide Documentation... the Penn Vector Core have been transferred to Addgene for distribution. Look up the IDs of particular...