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We narrowed to 954 results for: COMP

Showing: 561 - 570 of 954 results
  1. Video Library

    Type
    Protocol
    ...positions he has held in his career, his favorite company tradition, and Addgene's role in the scientific...
  2. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...In complex metazoans, rapid cell division and large scale cell mobility are essential processes during... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...organism. Studying cell mobility within these complex environments has never been easy and, for many ...labelling of cells using cre-lox is advantageous when compared to photoactivable or photoconvertible proteins...
  3. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...straightforward to learn and relatively cheap (compared to many other technologies and techniques in the...experiment work? What are the various tools and components necessary to run it? And, most importantly, what...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nature methods....
  4. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...fragments, allowing ordered assembly of multiple components into a destination vector. The first step in ...Oligonucleotides specifying the gRNA seed sequence should be compatible with type IIS enzyme BfuAI. Gateway cloning is...supply it with another plasmid.   Figure 3: Comparison of Multiplex Strategies including Standard PolIII-gRNA...Cas9-containing binary vectors. The system is compatible with both monocot and dicot plants. Liu Lab Golden...
  5. Sequencing Primers

    Type
    Guide
    ...from most sequencing services, along with a more complete list of common sequencing primers. These lists...
  6. Editor's Choice, July 2016

    Type
    Blog Post
    ...easier to generate mouse models.   Cpf1 Update: Comparison to Cas9 and NgAgo As you might expect given the...off-target editing and similar on-target editing when compared to the most commonly used Cas9 variant, SpCas9...Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison to Cas9 and NgAgo Special Delivery: Fluorophore...
  7. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...are of interest to you, our readers. Below I’ve compiled a list of the Top 10 Most Viewed Posts on our ...learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular, generating ...Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids 101: The Promoter...
  8. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...difficult to compare the performance of different nucleases. Nicola Patron’s lab compared the efficiency...more predictable and precise deletion products compared to monomeric Cas9’s. Read the paper in Nature...
  9. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...’d like to take a moment to reflect on our accomplishments from 2022 and share what we’re looking forward...ready-to-use recombinant antibodies collection, currently composed of over 100 antibodies. Everything in this collection...upon, a wealth of history to learn from, and a compelling future to plan in the changing landscape of science...
  10. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    ...tags with defined 3D structures less likely to compromise protein folding and function. Using bioinformatic...GFP did not reduce yeast cell size or viability compared to untagged GFP overexpression, while the rest...cell size and growth rate, indicating that they compromised protein-protein interactions. If you’re looking...
Showing: 561 - 570 of 954 results