...through Caitlin’s blood. “I come from a family of educators, mostly elementary school, middle school, and ...school, I actually served as the student course coordinator. With the guidance of one of the faculty members...their lecture, giving them advice, [and helped] proctor exams.” These teaching experiences led Caitlin ...interview experience.” Although luck wasn’t the only factor in Caitlin landing her job. All of the hard work...

... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...Addgene’s plasmid sharing services, as well as #happydepositors who stopped by to say hello. Needless to say...amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence...sphaeroides!) at the ASM booth. As well as being the Director of the Great Lakes Bioenergy Research Center and...

...How else can antibiotics be used in the lab? Historically, antibiotics have also been used to disrupt ...successful cloning. In these instances, the cloning vector typically has two separate resistance cassettes...powder form, but quickly breakdown in solution. Storing aliquots at -20oC and avoiding repeated freeze/...not add them to media hotter than about 55oC and store plates/stocks wrapped in foil if a light-sensitive...

... first step. Developing mentoring relationships, especially with peer mentors, to discuss your strengths...recently, I have been hearing exit survey data from postdoctoral programs in the Boston area that demonstrate...Both explicit and implicit aspects of today’s postdoctoral training can directly interfere with a seamless...

...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants ... more than 100,000 samples to researchers and educators in 60 countries each year. Introducing Arabidopsis...Science and Industry (COSI) and the Franklin Park Conservatory. Do you use Arabidopsis in your research? Are...

...after subtracting out non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins...the extracellular domain of the erythropoietin receptor and subsequently evaluated interactions with mutant...antibodies against MBP are commercially available. Factor Xa loves calcium, but metal-chelate matrices, not...Addgene.org Learn about Popular Plasmids in the Repository Learn about New Plasmid Technologies in our Hot...

...the table below for more details.   Common Vectors Copy Number+ ori Incompatibility Group Control ...Relaxed This table defines common cloning vectors, their copy number, ori, and incompatibility groups... ori's are referred to as phagemids.   Other factors that affect plasmid copy number  Although the...affect the copy number of a plasmid, other external factors contribute as well. These considerations are especially...

...). Similar to the goodwill of Addgene plasmid depositors, this growing resource of over 100 entries is...data prepared. All of these questions relate to factors that you should have already recorded as good reproducible...Chemogenetics) or light (Optogenetics) to activate receptors? If you’re in the process of designing your experiment...Addgene.org Read our AAV guide Browse all  AAV vectors Find ready-to-use AAV preps ...

... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...included a voltmeter on top of the battery box to monitor charge status and a disconnect switch between the... the battery bank and the vehicle battery/alternator to make sure the vehicle battery didn't run down.... can enable applications where reagents must be stored at elevated temperatures for long time periods ...

... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...such as ApE or commercial workbenches such as MacVector or Benchling. Utility functions: These functions.... For this reason I generally recommend that laboratories that have already attempted several CRISPR experiments...above or, even better, first-hand knowledge from laboratories employing CRISPR on the same organism could ...CRISPRseek offers great utility and coupled with Bioconductor should be the first choice for anyone already...