We narrowed to 997 results for: lars;

...believe this isn’t cause for concern. However, a large number of hits to one sequence/gene - we set our...Their in Vivo Persistence after Vector Delivery. Molecular Therapy 12:744–753 . https://doi.org/10.1016/j.ymthe...Single-stranded Virus Next-generation Sequencing. Molecular Therapy - Nucleic Acids 4:e260 . https://doi.org...Recombinant Adeno-Associated Viruses. In: Methods in Molecular Biology. Humana Press, pp 247–278 Additional resources...

... generate rAAVs in your own lab with standard molecular biology tools and tissue culture experience. Here...Recombinant Adeno-Associated Viral Vectors [Preprint]. Molecular Biology. https://doi.org/10.1101/2023.03.20.533580...cost-effective manipulation of gene expression in the CNS. Molecular Neurodegeneration, 15(1), Article 1. https://doi.org...B. J., Caton, T., Chiorini, J. A., Chtarto, A., Clark, K. R., Conlon, T., Darmon, C., Doria, M., Douar...

... close to the hood so you can view your cells regularly (both before entering the hood and when putting...identified. Even if some mammalian cells lines have similar morphologies, being familiar with these morphologies... the secretion of lactic acid (a by-product of cellular metabolism that is toxic to the cells), and need...Institute of Colloids and Interfaces. Sana is particularly interested in Glycobiology and Immunology. You...

... applied for jobs, I knew I should negotiate my salary after receiving an offer but even as I did (successfully... wearing business suits discussing multimillion dollar contracts behind closed doors. Fig. 1: The...prioritize in a lab with both electrophysiologists and molecular biologists? What is their perception of me and...working with you will benefit them too. Ed's note: Salary negotiations will be covered separately in a future...

...isolated, and disease-associated. Mobile-CRISPRi: a modular approach for CRISPRi transfer Mobile-CRISPRi offers...existing bacterial CRISPRi methods. First, its modular design allows for customization with strain-specific...generations. Figure 1: Mobile-CRISPRi's modular components. Image from Peters et al., 2019 with...be used in non-model bacteria species where its modular nature allows for the customization of guides, ...

...the use of degron tags has become increasingly popular for the modulation of endogenously and exogenously...uses a small molecule, a tag, and the endogenous cellular degradation machinery to function. The dTAG is...light up a tagged protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting...proteasome for degradation. The HaloTag is quite large (33 kDa) and does not self-cleave off of the protein...

...long as the disciplines have existed, cell and molecular biologists have been continuously pursuing new...Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still quite small... become one of the most commonly used tags in molecular biology. And the Myc1-9E10 antibody remains a ...experiments. It turns out that even with the most popular antibodies, context is key.    References and...

...More recently, online journal clubs have become popular, including those based on Twitter. At my former...interest from relevant parties, and the company at large, by polling and informal communications we made ...winding down. Perhaps other projects have taken on a larger priority for the participants. Perhaps people feel...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed...

.... Standard curves are critical for a number of popular scientific applications, and the quality of a standard...sample(s) in the same buffer if possible, or very similar buffers if not, since buffer components can affect...pipetting small volumes (< 2 µL) or volumes too large for a standard micropipette (> 1,000 µL). The standard...consistent dilution factors, change pipette tips regularly, and mix samples very well.  Use duplicates or...

...produce your own recombinant antibodies with some molecular biology and cell culture experience. Let’s go ...further processing. It may be helpful to incorporate regular feeds during this incubation time to maintain cell...cells, and debris. Affinity chromatography is a popular choice for this. Chromatography columns containing...Grimsley G, Gray T. How to measure and predict the molar absorption coefficient of a protein. Protein Sci...