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We narrowed to 971 results for: lars;

Showing: 561 - 570 of 971 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ... you rule out errors like uneven pipetting or irregular membrane transfer. Figure 1: A loading ...important to validate these assumptions for your particular experiment and choice of loading control. First...Ubiquitously expressed proteins like β-actin or GAPDH are popular choices for loading controls. These are often called...in many cases, you should validate it for your particular setup. We recommend starting by searching the...

  2. March for Science

    Type
    Blog Post
    ..., are limited by reality – a government cannot declare that gravity change direction and make things fly...comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists believe climate... supporting it.  3) Linking scientists to one particular political party while alienating the other While...or politicians to interpret it for them. Other popular reasons to march include: General inclusion of...

  3. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...classes and resources that can provide details and vocabulary for this skill. What this post may do is help...something should be communicated is necessary in a large organization, because confirming that every person... I don’t just rely on what I know about those particular teams at that given time. I think about how previous... previous iterations of those teams resolved similar issues in the past, or how completely different teams...

  4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... enough for all applications. To resolve this, similar systems have been developed for tracking various...animals only permits visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila...making this system useful for visualization of cellular extensions, such as neurons in the fly visual ...

  5. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...accomplishment while the longer tasks will give you larger payoffs later. Don’t be afraid to accomplish some...ve also recently started using Trello to manage larger, multi-person projects. Day-to-day, flexible to-do...below): Read new Zhang Lab Paper Find plasmids similar to those in the paper Ask Mary G. about her thoughts...how much time you can possibly handle doing a particularly annoying task and then dedicating that much ...

  6. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction ...bait protein can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing...29169465.  Ge, Yun, et al. "Enzyme-Mediated Intercellular Proximity Labeling for Detecting Cell–Cell Interactions...smaller biotin ligase for BioID proximity labeling. Molecular biology of the cell, 27(8), 1188-1196. PubMed ...

  7. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...genes for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency...Table 2 provides a quick reference for some of the popular strains, their genotypes, and their primary use...Chloramphenicol High competency cloning and propagation of large plasmids, ligated DNA, and libraries, nalidixic ...coli and others are model organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication The ...

  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ... factors of phages) constitute one of the most popular classes of phage proteins to study. These are highly...characterizing sugars and sugar-containing molecules in molecular biology labs (for a review, see Simpson et al.... without causing issues associated with cloning large fragments. After fragmentation, repair sheared ends... and a few proteases knocked out making them particularly useful for protein production), then dilute ...

  9. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    ... excited to announce the fourth edition of our popular eBook, Plasmids 101, is now available!   Plasmids...everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting tips. The...updated with twelve new sections, including our popular "Dimers and Multimers" post. It's also been re-...

  10. Antibodies 101 Series on TikTok

    Type
    Blog Post
    ...Our popular Antibodies 101 animation is becoming a TikTok series! Follow @addgene on TikTok for bite-...information! Link in bio. #STEM #antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene...Addgene We'll be posting these animations regularly from now until May, so follow us to get updates whenever...
Showing: 561 - 570 of 971 results