We narrowed to 1,073 results for: TOR;

... (NHEJ) and alternative end joining (alt-EJ). Inhibitors to essential components of both pathways have...events (Yang et al., Int J Mol Sci). Adding these inhibitors to your culture media prior to and after Cas9...introduction increases HDR, just make sure to remove the inhibitors within a day or two to not affect the viability... one step further, you can inhibit cell cycle regulatory proteins responsible for the transition out of...to fuse an HR protein to the nuclease. Early HR factors fused to Cas9 have been shown to increase HDR frequency...committing a break to performing HR before other factors were loaded onto the break, potentially shuttling...

...available by both the Jacob Corn and Alex Schier laboratories. Delivering Cas9-gRNA ribonucleoproteins  An...This technique utilizes Cas9 proteins harboring receptor ligands (C in the figure above), which result ... potential complications, there are additional factors to take into account with these types of experiments...and purification, which will require additional laboratory reagents, equipment, and expertise. Final thoughts...production of the Cas9 protein and a gRNA in your laboratory, once these have been acquired, genomic editing...Central PMCID: PMC4289409. 3. Rouet, Romain, et al. "Receptor-Mediated Delivery of CRISPR-Cas9 Endonuclease ...

...small-molecule inhibitors of a particular PPI. In such experiments, small-molecule inhibitors are tested ...because FRET depends on a number of hard-to-achieve factors. For example, FRET requires that fusion proteins...signaling, integrin signaling, and transcription factor interactions (Kerppola, 2006). One of the first...between basic leucine zipper and Rel transcription factors (Hu et al., 2002). This would be more difficult...library to screen PPIs among 450 transcription factors in live fruit flies. Although BiFC open-reading...

...Our story today starts with a bit of Addgene history... As you may be aware, Melina Fan, her brother ...get Addgene from a concept to an up and running repository. As they built the foundation for what Addgene...previous years, this event was held at Executive Director Joanne Kamen’s home- a testament to the familial... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities...

...bacterial world produce an assortment toxins to claim territory or kill competing microorganisms, but did you ...as a counterselectable marker in the destination vector. Successful cloning occurs when the ccdB gene is...unmarked genetic modifications in the yeast Pichia pastoris. By combining both positive and counter selection...induced by methanol. Once linearized, the delivery vector can be integrated into the genome to introduce .../sok toxin-antitoxin system, they were able to monitor lacZ activity over 48 hours in vitro without antibiotic...marker for unmarked genetic modification of Pichia pastoris." FEMS yeast research 9.4 (2009): 600-609. PubMed...

...yourself needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding...with unlimited time? Another strategy for your brainstorm session is to start with something completely... ideas that I have found helpful in my team brainstorming sessions.  Allow the group time to think about...This is why perfectionists tend to also be procrastinators.  If you fall into this category, get out of...our lab space, our protocols and even our Lab Inventory Management system. I hope these tips help you ...

...lysozyme to reduce basal expression levels; expression vector cannot have p15A origin of replication F- ompT...higher T7 lysozyme expression than pLysS; expression vector cannot have p15A origin of replication F- ompT...codons not normally used in E. coli. Expression vector cannot have p15A origin of replication F- ompT ...alleviates inclusion body formation. Expression vector cannot have p15A origin of replication fhuA2 ...Cis-repression of the E. coli T5 promoter (found on vectors such as pQE or similar), inducible under IPTG (...lac repressor on the pREP4 plasmid). Expression vector cannot have p15A origin of replication F-, Φ80ΔlacM15...

...signaling pathways. Rho guanine-nucleotide exchange factors (GEFs) activate their partner Rho GTPase, leading...to reveal even more insights. Super-duper lipid editor And if recruiting proteins to the cell membrane...phosphatidic acid signaling (such as Hippo, AMPK and mTOR pathways) and to introduce unnatural head groups...membrane or lysosomes and its activity can be monitored by phosphatidic acid probe GFP-PASS. B) Time series...Additional resources on the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org...Activity-based directed evolution of a membrane editor in mammalian cells. Nature Chemistry, 15(7), 7....

...know is your area code. Senators: To find contact information for your senators all you need to do is select...active research scientists (scientists who hold a doctorate degree, are currently working full-time, and have...edition. Cambridge university press, 1988. AAS: Historical Trends in Federal R&D NJ.com: Rush Holt: Lawmakers...Scott Pruitt says carbon dioxide is not 'primary contributor' to global warming The New York Times: With Trump...affected by Trump’s immigration ban Studies in History and Philosophy of Science Part: Democratic values...

...list from your current P.I., lab manager, or administrator containing all of the materials that you’ve ...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...negotiations can still go horribly awry when several factors aren’t taken into consideration. 1. Always focus...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...Happy Sciencing, Damien   Damien Wilpitz is a laboratory research management consultant and coach in Boston...research scientists create productive and manageable laboratory environments. His business skills enhance his...