...none are lost. Each Addgene library has its own depositor transformation and amplification protocol, but... a file in FASTQ format, a standard format for storing NGS reads and their corresponding sequencing quality...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...screening, and if you have any tips, input, or stories of interest about library use, let us know in the...

...University, and did postdoctoral work at Harvard Medical School and the University of Toronto. He is an author...quick turn-around time of Sanger sequencing have historically matched the needs of most molecular biology ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...

... at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity. Ideally, this number should be ...Concentration (ug/ml) = A260 reading x dilution factor x 50 ug/ml This method is quick and simple and ...like a PCR product). This method also gives an indicator of DNA or RNA contamination based on the presence...protocols and tips Find the best way to elute and store your plasmid DNA Learn about the different grades...

...Tools for checking grammar and jargon Hemingway Editor  This is a free web-based tool for detecting the... saved to your Mendeley library. The library is stored in the cloud, so you can access it from anywhere...you organize and cite papers. With the Zotero Connector, papers can be saved from Firefox or Chrome browsers...with others which makes it easier to work with collaborators. Zotero is a desktop based program that’s compatible...

...imaged together, you will need to consider the same factors as when choosing an individual fluorescent protein...previous blog post for more discussion of these factors). In addition, you will also need to choose fluorescent...infrared fluorescent proteins require biliverdin as a cofactor and so you may need to supplement your cells with...received his PhD in biophysics from UCSF in the laboratory of Ronald Vale, after which he was a fellow at...

...light activation, you can’t use fluorescence to monitor processes directly affected by light. Long-term...To make luminescent imaging a reality, Addgene depositor Takeharu Nagai and colleagues at Osaka University...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns...

... a novel TAL effector nuclease (TALEN) facilitates efficient knockout of HIV co-receptor CCR5. Nucleic...This post was contributed by Scott Findlay, a Postdoctoral Fellow at the University of Alberta. If you’... out of frame! Scott Findlay is currently a Postdoctoral Fellow at the University of Alberta. He is interested...

...creating a time-stamped research plan in a public repository before conducting the experiment (note: registrations...participating journals before a study is conducted. Editors and reviewers recommend changes, and if everyone...position of having robust, null findings that are notoriously difficult to publish.  With Registered Reports...implement authentic scientific research and as the Director of Advising in the Division of Life Sciences at...

...shared the paper with my lab since one of the AAV vectors in the paper seemed like it could help one of my...conference, whether you are attending in person or monitoring that conference via social media. The podcast...BLACKandSTEM Twitter community. Dr. Page shares her story of how one seemingly innocuous tweet would not only...you advance your research or career? Share your story in the comments below!   Additional resources ...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...comparison to the 62-90% error rate seen with Cas9 editors. The lab believes that this system could potentially...Development of a cleavage-free dCas9-based gene editor using microbial SSAPs. Image from Wang, et al, ...live cells remains a challenge due to multiple factors, including limitations of number of flourescent...