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We narrowed to 971 results for: lars;

Showing: 571 - 580 of 971 results

  1. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...cells for mycoplasma (Link opens in a new window) regularly Do not over or under-grow your cells. Thaw a new...well (0 µL lentivirus, above) are dying. Perform regular media changes and monitor the growth of the cells...selection. It is important to monitor these cells regularly and replace the cell media. Cell death by some...cells in the culture, so it is important to do regular media changes and maintain optimal growth conditions... or removed from the culture, check the cells regularly to confirm transgene expression. As polyclonal... individual wells become confluent, expand into larger vessels. A confluent well of a 6-well dish can ...

  3. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... the HNH and RuvC domains, which are named for similar nuclease folds found in other proteins. The C-terminal...it, so many researchers think the enzyme needs cellular recycling factors to release its handiwork (Sternberg...understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed with UCSF ChimeraX...DNA to read its sequence. Nature Structural & Molecular Biology, 29(4), 395–402. https://doi.org/10.1038...

  4. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...without dedifferentiating into a pluripotent stage. Cellular reprogramming The reversal of a differentiated...cells [41]. The in vivo and in vitro methods are similar except that the vectors carrying the transdifferentiation...transdifferentiation is suited for switching cell types between similar lineages, reprogramming is more versatile and universal...PMC3390875. 25. Patsch, C., et al., Generation of vascular endothelial and smooth muscle cells from human...

  5. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    ... here in New England- the majority of which are solar, wind, or hydroelectric in nature. The operation... electricity will allow us to maintain these particularly energy-intensive systems in a more environmentally...cartridges can place the spent cartridge in the large cardboard box in the copy area. These spent cartridges...

  6. Call for Guest Bloggers

    Type
    Blog Post
    ...expertise through our blog. Do you have skill in a particular technique? Do you have a set of tips you give... An overview of software tools useful to any molecular biologist Tips for protein purification Replica...Recent Guest Writers Have Included: Wenning Qin, Molecular biologist with Biogen interested in applying CRISPR...

  7. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ...fluorescent markers or protein tags, and suitable for molecular cloning. Plus, we decided to include 10 plasmids...µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments such as cloning, digestions... Learn about our other beta projects Read our Molecular Biology Reference ...

  8. AAV Production in HEK293 Cells

    Type
    Protocol
    ... Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol can...efficient means to scale-up without having to handle a large number of T-175 flasks. Workflow Timeline Day 0:... (PES) filter system, Nalgene, 565-0010 (or for larger volumes 1000 mL 0.45 µm Rapid-Flow PES Filtration...the amount of each plasmid needed to have a 1:1:1 molar ratio with 2 mg total DNA per CS5 Plasmid Plasmid...determine the total μg/bp we need to achieve a 1:1:1 molar ratio of each plasmid: 2000 μg / 24,961 bp = 0.08.... You are now ready to purify your prep . The clarified supernatant can be kept overnight at 4 °C before...

  9. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ... countries. Guides and protocols were our most popular content, while our beloved blog received 1.3 million...scientists in this rapidly changing environment. In particular, we are working to understand what challenges...Addgene colleagues, collaborators, partners, and the larger scientific community to maximize the positive impact...

  10. Water Bath Protocol

    Type
    Protocol
    ...equipment that helps bring your materials to a particular temperature, catalyze chemical reactions such...such as restriction digests needed in molecular biology, and thaw cell lines. There are many different types...properly maintain the water bath, empty and clean it regularly. Determine the amount of water needed to fill ...out after your incubation is over. Note: If you regularly use a water bath in the lab, you can leave the...previous uses. However, you will still need to regularly clean out the water bath even if you are using...
Showing: 571 - 580 of 971 results