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We narrowed to 938 results for: Kin

Showing: 581 - 590 of 938 results

  1. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...components of the indicator, which can make the kinetics of voltage sensing to signal output slightly slower...fluorescent signaler. The pro of this system is the kinetic response time from signal input to readable output...FP-rhodopsin GEVIs have been engineered to have kinetics comparable to its one component counterpart and...

  2. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ... I’m not going to lie, AAV transfer plasmids are kind of a pain to work with. These plasmids contain ITRs...the viral particles of the AAV serotype you’re working with are primarily found in the culture media, .... If you’re new to creating gradients, practice making the iodixanol layers before doing your first purification...

  3. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...the FP (fused to the protein of interest), thus tracking the localization of that biomolecule inside living...eel was shown to mature independently of oxygen, making suitble for use in anaerobic conditions. Maturation...fluorescent protein should I use?" And if you're looking for more fluorescence microscopy tools and techniques...

  4. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG...available nanobodies from Pleiner et al. Before picking a secondary nanobody, it’s important to review ...expression plasmids here Additional Resources Looking for monoclonal primary antibodies to use with the...

  6. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...our samples 1:6 million to 1:25 million.  After making the dilutions, they are transferred to a new plate...performing ddPCR Clean everything You must have a clean working area to titer your AAV by ddPCR. Droplet digital...generator, you should avoid pipetting too quickly when making your virus dilutions. This will reduce aerosols...

  7. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...does not do). Furthermore, molecular biologists working with vectors are, in most cases, interested in ... the viral integrase protein (IN) during step 3 (King et al., 2008) (Hu & Temin, 1990). At step 3, the....1016/0022-2836(73)90277-5. PubMed PMID: 4355596 King, S. R., Duggal, N. K., Ndongmo, C. B., Pacut, C....

  8. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...dye, mauvine, was discovered in 1856 by William Perkin. His discovery set off a flurry of activity and...labeling strategies and improved fluorophores are making chemical dyes increasingly attractive and accessible...Medical Institute. He would rather be in the lab making new fluorophores for advanced imaging modalities...

  9. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    ...through smart engineering Different FLP versions – making FLP efficient The first version of FLP discovered...proteins needed for later experiments (cellular tracking for instance) can be controlled with FLP-FRT. ... Cardin, Jessica A. et al. 2009. “Driving Fast-Spiking Cells Induces Gamma Rhythm and Controls Sensory...

  10. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...when 2 mismatches are present. The protospacer flanking sequence (PFS) for LshCas13a, which is analogous...have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating expression... in length and does not require a specific PFS, making it a very flexible cleavage system. LwaCas13a is...
Showing: 581 - 590 of 938 results