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We narrowed to 971 results for: lars;

Showing: 581 - 590 of 971 results

  1. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...without controversy, and it remains to be seen how popular any one of them will become. One thing is for sure...was... pSpCas9(BB)-2A-Puro (PX459) V2.0       Similar to last year, the top requested plasmid from 2016...peptides. PX459 This is the vector backbone. This particular backbone can be used to transfect a variety of...

  2. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...microscopes work as conventional microscopes and give a similar optical quality (magnification of 140X and 2 micron... to microbes by using non-invasive methods for cellular imaging with ultrasound. In his research he applies...applies naturally occurring bacterial “molecular buoyancy devices” (also known as gas vesicles) as acoustic...

  4. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...like GFP came to be used as research tools is particularly interesting. Although humans have known about...crystal jellyfish Aequorea victoria (Figure 1) in molecular detail. On the underside of the “umbrella” of ...Soon, the number of people studying it grew. In particular, studies in the laboratory of UCSD biochemist...

  5. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...Urbana-Champaign. The PUF proteins include eight modular repeats, each of which binds a consecutive base...on a version that she hopes will change the intracellular localization of RNA. “You can think of changing...the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins for specific ...

  6. Centrifugation

    Type
    Protocol
    ...microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending on the centrifuge...They often spin at similar speeds to their smaller cousins, but can hold much larger containers. Ultracentrifuges...generally floor sized centrifuges that can hold larger containers, but they can also rotate at much higher...

  7. Lentivirus ddPCR Titration

    Type
    Protocol
    ...viruses per cellular genome: Since the cellular gene RPP30 is used as a reference to the cellular genome, ...other targets. Primers and a probe against the cellular ribonuclease P/MRP 30 kDa subunit (RPP30) are ... lower or higher dilutions depending on their particular sample. This protocol was modified from the publication...#71205-3 Polybrene 10 mg/mL, Millipore, TR-1003-G Molecular Biology Grade Water, Hyclone, SH30538.02 ddPCR...

  8. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    ... responsible for cancer from genomics data, and large-scale patient cohort data from the MD Anderson Cancer...constructs using the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (Tsang et al., 2016... perturbations in vitro using Ba/F3 and MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent...

  9. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ...correct phages will test. These includes labs with a large number of different phages or with phages targeting...that phage information was very fragmented. A particular phage researcher may only know how to prepare...networking Additional resources on Addgene.org Find molecular biology protocols here Visit the Addgene careers...

  10. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...production of deletions by paired DSBs may be particularly useful. The first is the study of non-coding...and Harvard Medical School. His lab studies the molecular genetics of blood cell development and disease...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ...
Showing: 581 - 590 of 971 results