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...mutagenesis libraries or to insert heterologous genes, reporters, or other markers into a target genome. They did...screening New systems have been developed and deposited with Addgene which allow scientists to use CRISPR-Cas...Rep 2014 Jun 27;4:5469. MitoTimer plasmids to report mitochondrial turnover Mitochondrial health...Gottlieb’s lab has used an inducible MitoTimer reporter using a Tet-on system (pTRE-Tight-MitoTimer) to... to show its usefulness in cell culture to report changes in mitochondrial turnover and transport. pMitoTimer... Yan’s lab, which is constitutively expressed, reports on the balance of biogenesis and mitochondrial ...channelrhodopsins. CnChR1, nicknamed Chrimson, is the first reported yellow-peaked channelrhodopsin with a spectral...

...being requested or deposited. But, with our respective Master Reception and Master Deposit Agreements, your...future requests/deposits. This reduces the processing time of request MTAs and deposit agreements to basically...material requests and less than one week for material deposits. We were excited to deliver the very first ones... guarantee certain rights and protections for depositor and requestor alike. That’s why Addgene works ...works closely with TTOs to help protect our depositors’ intellectual property, and to help requesting institutions...Before Addgene can begin distributing a material, a deposit agreement must be put in place. And subsequently...about the Golden Stopwatch! Any organization that deposits with and/or requests materials from Addgene can...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These brief...posts for every great plasmid that comes into the repository, but be sure to let us know if you'd like to ...studying the nervous system The Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will...phosphatase-positive assay) as well as stabilization (Nanog-GFP reporter activation and silencing of factor-linked mCherry...are available at Addgene. Kim, et al. Stem Cell Reports. 2015. PubMed PMID: 25772473 Targeting and manipulating...enhancer elements and combined it with a variety of reporters (i.e. eGFP) and effectors (i.e. DREADDs). The ...imaging and in protein fusions. The Gadella lab has deposited all mScarlet constructs with Addgene. Bindels...

...fundamentally the reporter construct monitors the expression of a nearby gene. The reporter gene serves as... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...stimulate the expression of the reporter. The system is made up of a reporter gene flanked by a minimal promoter...promoter – all rely on the random integration of a reporter construct in close proximity to an endogenous ...example, if green fluorescent protein (GFP) was the reporter gene and green cells were observed in the kidneys... for with an uncharacterized gene. The actual reporter construct can be something phenotypic (such as...promoter. The system is composed of a promoter-less reporter which needs to integrate within an exon of a gene...

... Many of the systemic capsids in our repository are deposited by the Gradinaru lab. You can visit our...before moving on to deposits from other labs. AAVs not otherwise attributed were deposited by the Gradinaru...Look it up in Addgene’s Data Hub and the CLOVER repository of published systemic AAVs to find commonly used...coverage in rodent CNS Many of these capsids were deposited by the Gradinaru lab. For simplicity, in each ...AAV-PHP.B was the first systemic capsid developed and deposited by the Gradinaru lab (Deverman et al., 2016). ... the more efficient AAV-PHP.eB, developed and deposited by the same lab. AAV-PHP.eB is the most commonly...commonly requested systemic capsid in the Addgene repository. Like its parent AAV-PHP-B, it can be produced...

... upon you, our depositors, giving us as much information as possible during the deposit process. Special...amazing plasmid technologies contributed by our depositors, Addgene strives to make it easier for you to...plasmid" section Anytime you use a plasmid from the repository, we request that you cite it using both its Addgene...increases the visibility of both you and the original depositor. Our citations feature makes it so that, when ...you to browse through the many plasmids in the repository, our scientists have curated a number of special...attention to the great variety of plasmids in the repository. And last, but certainly not least, we often ...post about a plasmid or set of plasmids you’ve deposited with us. Don't forget to get yourself out there...

... transformation. Choose good reporter genes There are many great reporter genes available and which one...the T-DNA region with our gene of interest or a reporter gene so that the transformation and gene expression...different treatments on transformation success. Some reporter gene examples are below. The GUS gene (β-glucuronidase...histochemical GUS-staining is a very useful visual reporter (Figure 2). The GUS enzyme cleaves a substrate...transformation success. Luciferases are great quantitative reporters and use a substrate to produce bioluminescence...half-lives then GUS and are therefore good temporal reporters to measure promoter activity, for example. Useful...Agrobacterium. This is especially problematic with reporter genes as you might observe expression from Agrobacterium...

...quality cell culture. Biorepositories around the world ensure that the cell lines deposited with them and used...validation carried out by the commercial repository. Repositories generally ensure cell line identity and...invariably much older than those available from biorepositories leading to genotypic and phenotypic artefacts...been known about since 1966 when Stanley Gartler reported that most of the 18 cell lines he had studied ...The oldest, largest, and most diverse of these repositories is the American Type Culture Collection aka ... between materials available from commercial repositories and the same cell line from a colleague?”. The...cells lines. Even if you get your cells from a repository like the ATCC, there are times when it is important...

...It’s nearing the end of #DepositorWeek at Addgene and we’d like to show our gratitude on the blog by ...highlighting some of the great posts written by Addgene depositors. Below you’ll find brief summaries of several...several excellent guest posts from our depositors. Interested in writing your own post for the Addgene Blog...Lab at MIT developed plasmid based systems that report the order in which specific chemical inputs are...recombinase-based state machines could potentially be used to report upon and/or engineer “temporally ordered biochemical...derived from Zika virus and, upon binding, produce reporter proteins. These toehold switches can be used to...viral replication and transcription as well as reporter genes. To learn how you can use minigenomes in...

...luciferases, which have become common genetic reporters and alternatives to fluorescent proteins. ...Created with BioRender.com. Bioluminescent reporters require a chemical substrate (and sometimes cofactors...signal sensitivity. Another advantage: luciferase reporters aren’t susceptible to photobleaching — they’ll...firefly luciferase was the first bioluminescent reporter to be cloned and remains popular, other luciferases...beetle (Pyrophorus plagiophthalamus), deep-sea copepod (Gaussia princeps), Japanese firefly (Luciola cruciata...or naphthyl-luciferin analogs ATP No Deep sea copepod (Gaussia princeps) Natural 480 20 Coelenterazine...Engineered   677 61 AkaLumine ATP No Luciferase reporter assays for monitoring gene regulation Since firefly...