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We narrowed to 132 results for: multiplex

Showing: 51 - 60 of 132 results
  1. Viral Vectors 101: Calcium Sensors

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    Blog Post
    ...binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your calcium reading with ...you need to free up crowded optical space for multiplexing, GECO sensors are available in green, blue, ...
  2. Antibodies 101: Conjugation

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    Blog Post
    ... the visible spectrum, so they are great for multiplexing and can be used in a variety of assay types....detection of low abundance targets with significant multiplexing potential. You will find antibody-oligonucleotide...
  3. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ... 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate cloning...Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular Cloning...
  4. The Twelve Days of CRISPR

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    Blog Post
    ...the twelfth day of CRISPR, Addgene gave to me: multiplexed gRNAs.  We hope you’ve been enjoying our CRISPR...
  5. Sequencing Options for CRISPR Genotyping

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    Blog Post
    ...counts making them fast and viable options for multiplex sequencing in many laboratories. Nevertheless,...DSBs, doesn't require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic...dsODN), doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to...
  6. When Fidelity Matters: A frank discussion about ligase fidelity

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    Blog Post
    ...detection (14-20). LDR has also been extended to multiplexed probe sets that allow the simultaneous interrogation...extends earlier studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used...: PMC148763. 15. Pingle, Maneesh R., et al. "Multiplexed identification of blood-borne bacterial pathogens...
  7. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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    Blog Post
    ...cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously produces ... Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on Addgene.org...
  8. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post
    ...vectors. In 2017, Zetsche et al. developed a Cpf1 multiplexing approach using a single crRNA array to express...publication at Addgene. 2. Zetsche, Bernd, et al. "Multiplex Gene Editing by CRISPR–Cpf1 Using a Single crRNA...
Showing: 51 - 60 of 132 results