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We narrowed to 728 results for: STO

Showing: 151 - 180 of 728 results

  1. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...structures (at < 5 Å resolution) of the linker histone H1.8 in Xenopus nucleosomes and in complex with...has developed a new epigenetic editor, Coupled Histone tail for Autoinhibition Release of Methyltransferase...can be toxic, CHARM uses a cofactor fused to a histone tail to recruit and stimulate the endogenous methyltransferase...uses a fusion of the DNMT3L cofactor (D3L) with a histone tail (H3K4me0) to a DNA-binding protein (dCas9 ...team to develop self-silencing CHARM variants that stop their own expression after silencing their target... a familiar challenge for anyone who has done histology on brain tissue is identifying the cells you are...

  2. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    .... We are the Customer Support Specialists here at Addgene. If you’ve called our customer service line ...'Regan Ordering jeans online from your favorite store is easy. Ordering genes on plasmids online is a ...really getting at one bigger question: How does a customer place an Addgene order using a PO number? Why ...full of information about how to deposit plasmids, store your plasmids or viral items, technical support,...

  3. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ... Center for Regenerative Medicine of Boston University and Boston Medical Center. Special thanks to our...director of the Center for Regenerative Medicine at Boston University Medical School. Darrell’s research focuses...Chapter 1: Introduction to Darrell Kotton and the Boston University Center for Regenerative Medicine. Darrell...Center, and the Pulmonary outpatient clinic, all at Boston Medical Center. In addition he conducts basic stem...

  4. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...resistance gene (missing the first two codons). Two stop codons are inserted between the truncated gene and...ligated into the MCS/SacI site in pUCXKT, removing the stop codons during backbone digestion and providing the...Toolkit for constructing fusion proteins with customizable N- and C-terminal modules   Thanks to the lab...Technologies. The MultiSite Gateway Kit is a customizable Gateway®-based toolkit that allows scientists...bound to the mRNA since they are tethered AFTER the stop codon. Thus a non-translated mRNA will appear yellow...: 10.1126/science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector...LacZ, puromycin or CreERT2 can be combined with custom-cloned vectors in a Multisite Gateway reaction ...

  5. An Addgene Summer Internship

    Type
    Blog Post
    ...my training, I dove straight into my capstone project. A capstone project is a major project given to ...Figure 1: Addgene's summer interns presenting their capstone projects during the Summer Intern Poster Presentation... scientist. But I expected something wrong. My capstone project was based on plasmid cloning and I, the... Once everything was confirmed, I worked on my capstone project report and presentation, during the final...

  6. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...you questions. Remember that people like a good story and science involves characters – in this case you...or other places online, and consider how those stories are being told. Twitter can be a good way to follow...surprised to find that his friends and family finally understood why he cared to study mice on some distant island...the East Coast, there’s programs at both MIT and Boston University. At Addgene we're committed to scientific...sharing – both your plasmids and your research stories. Consider practicing communicating your research...

  7. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    ...IP and ChIP assays. Tag 96 genes at a time using Custom Synthetic cr:tracrRNA/Cas9 Ribonucleoprotein (csRNP...first step of optimization, we started off by using custom synthetic two-part cr/tracrRNAs for CRISPR experiments...tagging, pick a crRNA that cuts 8-15 bp downstream of Stop codon; for N-terminus tagging, a crRNA cutting 8...csRNP experiments, it can be ordered in bulk and stored in small aliquots at -80 oC. Assemble csRNP: Anneal...near the insertion site (within ~8-15 bp from the stop codon for C-terminus tagging) GC content of the ... hands, delivering Cas9 protein in complex with custom synthetic cr:tracrRNAs (csRNP) is the most efficient...

  8. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...not necessarily represent the views of Addgene. Boston, MA, USA 42.3601°N 71.0589°W – Alina Chan The ...dabbles in science policy, public outreach, and restorative justice.  Follow her on twitter @sung_vanessa...to join the public conversation and to tell the stories behind our research.  That's why I marched for ...Michigan, where programs like the Great Lakes Restoration Initiative are at risk of major cuts and where...behind these two fingers the ninety-nine cubits of Aristotle, nor would you mention my small error and at the...second and that it is our duty to tell our science stories in a manner that encourages open discourse with...

  9. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...Emission Depletion (STED), and Stochastic Optical Reconstruction Microscopy (STORM). These dyes have specific...Throughout their history, fluorescent dyes have enabled the visualization of both organic tissues and...than was absorbed. This phenomenon is known as the Stokes Shift, or the difference in wavelength between ...technologies in fluorescent dyes/ligands Throughout the history of cell biology, fluorescent labeling techniques...fluorescent protein Fluorescent Proteins 101: A History of Fluorescent Proteins ...

  10. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...skeptical reader of historical fiction (I can personally attest to this!). Following history from the Ice Age...Wrong - and the New Research That's Rewriting the Story by Angela Saini "This book is a smart and carefully...Strange & Mr. Norrell by Susanna Clarke "A quasi-historical/fantasy fiction. Two dueling magicians in the...of England by Edward Rutherfurd "A 10,000 year history of southern England. While the length may be intimidating...

  11. GPCRs: How Do They Work and How Do We Study Them?

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    Blog Post
    ... examples with plasmids deposited at Addgene.  PRESTO-TANGO: The ligands that activate many GPCRs are...Roth’s lab created an open source resource called PRESTO-TANGO (parallel receptorome expression and screening...transcriptional output - TANGO) (Kroeze et al., 2015). PRESTO-TANGO uses a modified Tango beta-arrestin recruitment...ligands for unknown GPCRs. You can find the entire PRESTO-TANGO kit at Addgene! TRUPATH: Bryan Roth’s lab...McCorvy JD, Giguère PM, Sciaky N, Roth BL (2015) PRESTO-Tango as an open-source resource for interrogation...

  12. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    ...initial reversal drive cannot restore the exact original sequence, it can restore the original protein-coding...are eliminated by natural selection, gene drives distort inheritance in their favor and consequently can...they should not be relied on exclusively because historical studies of pathogen research have conclusively...using a recoding strategy; a subsequent drive can restore the wild-type sequence (save for the residual sgRNAs...

  13. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Fast-FT, and mK-GO. Large Stokes Shift (LSS): Stokes shift (named after George G. Stokes) is the shift in wavelength... most FPs, Stokes shift is less than 50nm (often much less).  For LSS proteins, the Stokes shift is ≥ ...

  14. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Ampicillin Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp ... factor. Zeocin is handy because it can be a one-stop shop for selection across systems and species. The...entirely detoxified, even when Sh ble is expressed (Trastoy et al). Thus, it may not be a good choice for experiments...Hadley, W. K., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial...11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O., Defais, M., & Larminat, F. (2005). Resistance...

  15. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... wrong. It brought the whole field to a grinding stop and only now, 2 decades later, is it finally back...showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot, one ... 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector for ... again 3. Acland G. et al., 2001. Gene therapy restores vision in a canine model of childhood blindness...PubMed PMID: 11326284. 4. Bennett J, 2017. Taking Stock of Retinal Gene Therapy: Looking Back and Moving...

  16. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    ...when balancing a handful of demanding courses, a capstone paper I really wanted to hit out of the park, .... I need it just as much in my current role as Customer Support and Operations Manager at Addgene. In ...about to keep silent and move on to other things…. Stop! Take a step back and think of a time you’ve lead...collaborator or co-worker and showed that you really understood their side of an issue. Maybe you also showed...article from The Muse. Kai Strickland is a former Customer Support and Operations Manager at Addgene and ...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...celebration, we thought it only fitting to share some stories of ways we’ve turned the available into the accessible...countries, import and export regulations, differing customs processes, and the distances the packages needed...other things, could ensure our packages navigated customs and arrived at their destination in a timely manner... to Design a Primer Creating Bacterial Glycerol Stocks Inoculating a Liquid Bacteria Culture Collections...scientists, from techs to grad students to professors, stopping by to say, “Everything I know about virus, I learned...

  18. Bioinformatics at Addgene

    Type
    Blog Post
    ...chose needed to fulfill certain requirements: Customizable to our specific needs Modern and well-supported...the order and usage of the scripts needs to be customized in the pipeline for the specific needs of the...Since each process can use a unique container, we customized each container for its particular purpose and...bbduk process.    At first, to make sure we understood the concept of channels and implemented the input...user interface. In our next post, we’ll share our story about how we used Nextflow Tower to augment our ...

  19. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...yourself needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding...with unlimited time? Another strategy for your brainstorm session is to start with something completely... ideas that I have found helpful in my team brainstorming sessions.  Allow the group time to think about...position or the longest tenure, but don’t let that stop you from speaking up.  If your ideas are not well...

  20. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    ...Stochastic multicolor labeling is a popular technique in neuroscience and developmental biology. This... persistently fluorescent during development. Stochastic multicolor labeling systems, many based on Brainbow...for improving the level of detail possible for stochastic multicolor labeling of the brain. Labeling Drosophila...express the same XFP transgene in an adult fly. As a stop cassette is located directly upstream of the fluorescent... researchers who developed Tetbow. Conclusion Stochastic multicolor cell labeling systems allow single...

  21. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...situ hybridization histochemistry: use for mRNA detection.” The Journal of Histochemistry and Cytochemistry...interaction has also been adapted for use in immunohistochemistry and antibody labelling. In these experiments...referred to as the Avidin-Biotin Complex (ABC) immunohistochemistry (IHC). For more information on ABC and IHC...Cytochemistry : Official Journal of the Histochemistry Society, 41.12 (1993): 1751–1754.PubMed PMID: 8245422. Additional...

  22. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...proteins from E. coli, Bacillus subtilis, or Pichia pastoris depending upon your specific protein folding needs...alleles in microbial populations through time, Christopher Marx's lab has engineered and validated a new...one-pot reaction. Using this system, you can create custom RNA binding proteins in as little as 3 days. Abil...Abil et al., 2014 show that you can fuse custom PUF proteins to a translational repressor to down-regulate...Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities." Nature communications...

  23. Evolution of Lab Techniques

    Type
    Blog Post
    ...papers or are brainstorming new technological innovations. Let’s take a look at three historical techniques...that save both time and money. Yet, knowledge of historical techniques comes in handy whether you are perusing...throughout the world. Let us know your favorite "Historical Lab Technique" in the comments section below!...Benjamin Chain. "The sequence of sequencers: The history of sequencing DNA." Genomics (2015). PubMed PMID...

  24. 5 Science Rap Videos & Their Creator: Tom McFadden

    Type
    Blog Post
    ...Science History Rap Battle A great example of science story telling following the unfortunate story of Rosalind...and their importance in understanding biological history. 4. Please Don't Kill My Hive Beautifully filmed...

  25. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...the research community. Customers have taken advantage of the customizability, choosing the cargo, serotype... ship them out in record time.  However, our in-stock preps remain a popular choice thanks to the convenience...) Looking for more viral vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request...

  26. Important Considerations When Using AAVs

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    Blog Post
    ...Location, Location, Location! Failure to Plan (for Storage) Is Planning to Fail Patience Is Bitter but Its... the transport issues. 2. Failure to plan (for storage) Is planning to fail Freeze/Thaw Cycles Of course...cycles can still reduce AAV titer. For long term storage, make > 25 μl aliquots in 0.5 ml tubes, snap freeze... liquid nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be... PubMed Central PMCID: PMC3098627. 6. Salegio, Ernesto A., et al. "Axonal transport of adeno-associated...

  27. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    ...documenting your plasmids, storing them, providing quality control and customer support, making plasmids...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...The trust of our depositors to allow us to help store and distribute their plasmids. The partnerships ...

  28. Typing CRISPR Systems

    Type
    Blog Post
    ...the CRISPR types and is thought to be the common ancestor of all other CRISPR systems. Type III systems ...(like me!) of CRISPR types — weird and poorly understood. There are three subtypes, A–C, and they are ...mechanism of type II is, unsurprisingly, well understood, and the first taught when introducing CRISPR...Covarrubias, P. C., Shmakov, S. A., Silva, C., Tolstoy, I., Johnson, D. B., Koonin, E. V., & Quatrini,...., Hunnewell, P., Alfonse, L. E., Carte, J. M., Keston-Smith, E., Sothiselvam, S., Garrity, A. J., Chong...

  29. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...equilibrium” where the cancer stops growing, and the immune system stops fighting. Such quiescent cancer...study cancer from every possible angle to leave no stone unturned, but cancer has been producing landslide...landslide after landslide, only adding more stones to the field. Fortunately, our bodies have been fighting ... downregulate the expression of the major histocompatibility complex (MHC) class I proteins on their surfaces...

  30. Reflections and Looking Ahead

    Type
    Blog Post
    ...shared resources. As we step into 2025, these milestones remind us of the extraordinary impact that happens...shared progress. But numbers tell only part of the story. This year, we made significant strides in making... D., Cooper, J. A., Crosby, K., Harvey, K. J., Houston, D. W., Kumaran, R., Rego, M., Schofield, C., …...024-01095-8 Kahn, R. A., Virk, H., Laflamme, C., Houston, D. W., Polinski, N. K., Meijers, R., Levey, A....
Showing: 151 - 180 of 728 results