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We narrowed to 753 results for: ELP

Showing: 201 - 240 of 753 results

  1. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    ...things go wrong, ask yourself, “What next step will help me achieve my purpose? Should I go over this slide...the three tips above to make a strategy that can help. Even something as simple as telling yourself, “... or “It is okay if it doesn’t go perfectly,” can help. (And do remember to breathe!)   Public speaking...

  2. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...The Rare Genomics Institute BeHEARD (Helping Empower and Accelerate Research Discoveries) Challenge provides...SLC6A8 deficiency. “This proof of concept study will help in identification of new targets that could greatly...release in keratinocytes,” says Mellett. This would help the lab identify therapeutic targets in the future...

  3. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ...Clarify: Asking for clarification during a meeting can help get everyone on the same page. Others in the meeting...might also have the same question and asking can help the team identify possible points of confusion in...measures in improving cross team communication has helped Addgene move from completing 2-3 large projects...

  4. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    ...obvious, in that a lot of science communication is helping people understand new concepts. You hopefully are... your audience knows, but the following tips can help:  If there’s a teacher, mentor, or other educator...target audience. Thinking about cognitive load will help you understand why you’re not communicating as clearly...

  5. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...over the years with the hope that our insights can help steer you in the right direction when writing your...examples are intentionally trivial, but they're here to help tell this story. In this first example to the right...Addgene and is interested in writing software tools to help scientists. You can find him on Twitter @krisshamloo...

  6. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ...symposium we will discuss where better data is needed to help shape and inform the overall structure of the biomedical...negative, of changing the workforce structure? Helping to answer some of these questions, and posing some...particular about postdocs. Among other involvements, he helped to establish grants through the Sloan foundation...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...deficient in E1, E2, E3, and E4. Gutless Ad vectors (helper-dependent (HD-Ad), or high-capacity (HC-Ad__ are...packaging signal in cis. Therefore, co-infection with a helper adenovirus is required to provide the viral proteins... production is more complex and the presence of helper virus contaminants have slowed the use of this ...

  8. Lab Automation at Addgene

    Type
    Blog Post
    ...modified organisms. Since Addgene is very proud to help scientists share plasmids across the globe, we’re...generate far more legible labels than your hand and help keep things more organized. Cap tubes with a micronic... it isn’t electronic but it is one of the most helpful machines I have used in the lab. It’s especially...

  9. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...only a great form of scientific outreach, it also helps you to hone your writing skills and make a name ...Science Writers Association, EUSJA or ABSW for helpful tips and job offers. If you live in the UK, join...taking a media or journalism course. Courses can help you gain work experience and build your network,...

  10. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...assembly design (DAD) to build out three tools to help researchers design high-fidelity Golden Gate Assemblies...accomplished with Golden Gate Assembly—even when getting help from DAD. The 35-piece assembly mentioned above ...deposited by NEB – and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R...

  11. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...addition to human error, fragments of DNA from the helper plasmids or the cell genome can be packaged inside...genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., ...to discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality...

  12. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...website for any relevant hashtags and handles to help you find other scientists attending. Reach out if...the conference hashtag to set one up. It’s also helpful to write your Twitter handle on your nametag for...are not looking for a job - those connections can help you later on. Update your LinkedIn and your resume...

  13. Antibodies 101: Flow Compensation

    Type
    Blog Post
    ...decrease the compensation value. If neither of these helps shift your population into the correct spot, you...markers can be challenging! But we hope this guide can help you get started. Remember, you can always change...finalize your compensation values later. This can be helpful as you're learning! Ashlyn has a research background...

  14. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...Where is the CRISPR community?  Addgene exists to help researchers share useful tools, and as such, we’...challenging. Addgene’s free educational resources help scientists navigate that learning curve. As of 2023...Genome Edit has 76,011 views.  Has all of this work helped scientists use CRISPR technology? We think so —...

  15. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...interest — for example, AAV8 for kidney cells — can help improve infection rates in your experiment. ... screen to a high degree. This is particularly helpful when investigating tissue-specific diseases, as...over lentivirus in many mouse tissues. This will help improve your selection pool for downstream NGS and...

  16. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ... bullet points for what you would like to say to help build your presentation. For example, when thinking... fundamental takeaway. Writing these points will help you remember what to say and provide your audience...Having them reframe or elaborate their thought will help you to provide an on-topic answer by creating time...

  17. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    ...Alignment Search Tool (BLAST) provided by NCBI. BLAST helps us compare the sequencing results of the plasmids...differences in the blastn programs, check out the BLAST Help webpage. Optimizing blastx searches Once you have... on our suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification...

  18. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ... recruit parents from any background, it really helps if activities are run by parents who can answer ...scientist. I think that establishing this history helped demonstrate that a) I was a real person who used... it worked. I came up with an analogy that would help them grasp some of the key concepts (Fig. 2). One...

  19. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...recurring meeting amongst scientists that aims to help stay current on new and emerging work by collaboratively...unable to attend for scheduling conflicts. This helped us find a time that accommodates more people. The...(multiple choice/binary) and open questions is helpful to ensure acquisition of the essential data and...

  20. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...productive and happy employees. The teambuilding culture helps us build relationships and thereby work better together.I...had in an orderly and timely fashion. 9. Ask for help My grandma used the old adage “many hands make light... lot of set up or cleanup, ask for volunteers to help out. Delegate- it will save you time and sanity!...

  21. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ... to submit an updated protocol? Just email us at help@addgene.org and we will be happy to upload your ...every lab's needs. Plus there are the apps - many to help you access your protocols at the bench, keep your...protocols show up on the related plasmid pages and can help scientists use these plasmids more successfully....

  22. Lab to Office Culture Shock

    Type
    Blog Post
    ...looking to see if there are any resources that can help with the transition.” The lab-to-desk-job culture...formal training regime and the right set of tools to help you do your job as well as possible. It’s in their... is an amazing thing to be a part of and really helps me get behind a project or initiative.” If you think...

  23. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...These demo details help us see the problem from the user's perspective which in turn helps us decide how to...software design choices, resulting in software that helps our users focus on the fun stuff, accelerating research...

  24. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ... can help you hop, skip, and jump between all kingdoms of life. A bit of careful design will help make...include viral oris to drive amplification, which helps increase cellular uptake, but this approach requires...

  25. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...GoogleScholar, Academia, ORCID, and MyScienceWork all help us share research. Open access journals also provide... by Scott Spicer at the University of Minnesota helps illustrate this: “In terms of total view count and...are some resources: ImpactStory, a website that helps researchers go beyond citation rates to measure ...

  26. Changing Labor Laws Bring Increased Postdoc Wages

    Type
    Blog Post
    ...At Future of Research, our volunteers have been helping to gather information for our FLSA and postdocs... us if you have information that you think would help our resource. In the medium term, it is unclear ...in advocating for and with junior scientists, to help them navigate the scientific enterprise and promote...

  27. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...pretty nicely displays her energetic, truthful and helpful style: Joanne also provided much more detailed...content for scientists in training is abundant, helpful, and sometimes excellent. Finally, as I said in... have questions? I am easy to reach and happy to help. Additional Resources on the Addgene Blog: 10 ...

  28. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...the functionality of your experimental system and help identify opportunities for troubleshooting or optimization...are control plasmids? Generally, control plasmids help to ensure that the observed phenomenon in your experiment...phenotype associated with the independent variable and helps ensure the phenotype isn’t singular or associated...

  29. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...’s best efforts. We all have software tools that help us efficiently accomplish our daily goals. These...electronic lab notebooks into 9 questions that will help you start and define what exactly your lab needs... 21 CFR part 11, HIPAA? Answering this question helps you evaluate whether the ELN should have functionalities...

  30. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...offering advice on areas where you might need some help. Today in our transferable skills guide: Time Mangement... made small accomplishments each day that should help keep you content and give you the energy to finish...day, writing your to-do list the night before will help keep you focused. For a real-world example, let...

  31. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    ...step can involve multiple smaller steps that all help to ensure that antibodies can access their target...number of websites compiling antibody data that can help you compare antibodies from different companies,...Sharing your experience, positive or negative, will help make antibodies more effective and reliable in the...

  32. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...plasmid, into which the GOI is inserted, and a Ti "helper" plasmid, which contains Vir genes necessary for...insertion into the plant genome. B) The Ti plasmid and helper are transformed into agrobacterium tumefaciens ...(yellow) are recognized by the Vir genes on the helper plasmid and used to insert the intervening region...

  33. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ... set of plasmids together on a tight budget. To help popularize our kit resources,  we’re highlighting...selection markers built into all plasmids in the kit help decrease false positives observed in the cloning..." Small GTPases 7.2 (2016): 93-106. FREQ-Seq To help scientists track the frequencies of specific alleles...

  34. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... to quantify accurately, but can be especially helpful to generate more signal from low abundance targets...individual panels, then the multicolor overlay; this helps all viewers, especially if you have some complex...improved variations you could try. We hope this post helped you start planning for your next multiplex IF experiment...

  35. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...of known molecular weight in a separate lane can help confirm the size of the protein of interest. An ...  Visualizing your protein of interest with the help of secondary antibodies In order to visualize proteins...in your specific system. If you found this blog helpful be sure to check out Addgene’s blog for overviews...

  36. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    ...rate of the vector you are using. Contact Addgene (help@addgene.org), or the supplier of your vector, to...before beginning large scale experiments. This will help to ensure the accuracy and reproducibility of the...Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations. Front Synaptic Neurosci 12...

  37. Finding Your Science Policy Path

    Type
    Blog Post
    ... meet ups and happy hours that you can attend to help build relationships.  Reach out to a science policy...important question that you can explore. The answer will help you decide how to define the who, what, where, when...challenges you’ve overcome. This information may help you determine what parts of your current job you...

  38. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...you need help putting your list together. Once you have your list of genes, Green Listed can help make the...CRISPR screen. Luckily, the Green Listed software can help you do just this, probably in less than a minute...

  40. New Antibody Animation!

    Type
    Blog Post
    ...about antibodies? Our latest animation is here to help! Join Abi as they explain what antibodies are, their...Subscribe to Addgene's YouTube channel for more helpful animations, useful protocol videos, and quick tips...
Showing: 201 - 240 of 753 results