We narrowed to 755 results for: ELP

...being alike. At the time when we spoke, Susan was helping cover social media while the Hutch hired a full-time...work for paid work: “If you use it right, it could help you get your name out for stuff that you’ve written...scicomm career: “ I think living in Washington D.C. helped because there was so much work through the federal...

...things go wrong, ask yourself, “What next step will help me achieve my purpose? Should I go over this slide...the three tips above to make a strategy that can help. Even something as simple as telling yourself, “... or “It is okay if it doesn’t go perfectly,” can help. (And do remember to breathe!)   Public speaking...

...Clarify: Asking for clarification during a meeting can help get everyone on the same page. Others in the meeting...might also have the same question and asking can help the team identify possible points of confusion in...measures in improving cross team communication has helped Addgene move from completing 2-3 large projects...

...score ~99.9% helpful and the customer and technical service teams put 100% effort into helping our customers...internal referrals. Our long reaching reputation helps us hire great people from farther away via connections...

...nifty technique, but what kinds of questions can it help answer? Ramanathan et al. outline three applications...breast cancer. Infectious Disease. RaPID can also help identify which host proteins interact with viral...interactions. Do you have a question that RaPID could help answer? The RaPID plasmids are available from Addgene...

...over the years with the hope that our insights can help steer you in the right direction when writing your...examples are intentionally trivial, but they're here to help tell this story. In this first example to the right...Addgene and is interested in writing software tools to help scientists. You can find him on Twitter @krisshamloo...

...symposium we will discuss where better data is needed to help shape and inform the overall structure of the biomedical...negative, of changing the workforce structure? Helping to answer some of these questions, and posing some...particular about postdocs. Among other involvements, he helped to establish grants through the Sloan foundation...

...only a great form of scientific outreach, it also helps you to hone your writing skills and make a name ...Science Writers Association, EUSJA or ABSW for helpful tips and job offers. If you live in the UK, join...taking a media or journalism course. Courses can help you gain work experience and build your network,...

...modified organisms. Since Addgene is very proud to help scientists share plasmids across the globe, we’re...generate far more legible labels than your hand and help keep things more organized. Cap tubes with a micronic... it isn’t electronic but it is one of the most helpful machines I have used in the lab. It’s especially...

...assembly design (DAD) to build out three tools to help researchers design high-fidelity Golden Gate Assemblies...accomplished with Golden Gate Assembly—even when getting help from DAD. The 35-piece assembly mentioned above ...deposited by NEB – and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R...

...deficient in E1, E2, E3, and E4. Gutless Ad vectors (helper-dependent (HD-Ad), or high-capacity (HC-Ad__ are...packaging signal in cis. Therefore, co-infection with a helper adenovirus is required to provide the viral proteins... production is more complex and the presence of helper virus contaminants have slowed the use of this ...

...interest — for example, AAV8 for kidney cells — can help improve infection rates in your experiment. ... screen to a high degree. This is particularly helpful when investigating tissue-specific diseases, as...over lentivirus in many mouse tissues. This will help improve your selection pool for downstream NGS and...

...decrease the compensation value. If neither of these helps shift your population into the correct spot, you...markers can be challenging! But we hope this guide can help you get started. Remember, you can always change...finalize your compensation values later. This can be helpful as you're learning! Ashlyn has a research background...

...Where is the CRISPR community?  Addgene exists to help researchers share useful tools, and as such, we’...challenging. Addgene’s free educational resources help scientists navigate that learning curve. As of 2023...Genome Edit has 76,011 views.  Has all of this work helped scientists use CRISPR technology? We think so —...

...addition to human error, fragments of DNA from the helper plasmids or the cell genome can be packaged inside...genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005, Wright et al., ...to discuss. We are also available by phone or at help@addgene.org to answer your questions about our quality...

... bullet points for what you would like to say to help build your presentation. For example, when thinking... fundamental takeaway. Writing these points will help you remember what to say and provide your audience...Having them reframe or elaborate their thought will help you to provide an on-topic answer by creating time...

...website for any relevant hashtags and handles to help you find other scientists attending. Reach out if...the conference hashtag to set one up. It’s also helpful to write your Twitter handle on your nametag for...are not looking for a job - those connections can help you later on. Update your LinkedIn and your resume...

... to submit an updated protocol? Just email us at help@addgene.org and we will be happy to upload your ...every lab's needs. Plus there are the apps - many to help you access your protocols at the bench, keep your...protocols show up on the related plasmid pages and can help scientists use these plasmids more successfully....

...Alignment Search Tool (BLAST) provided by NCBI. BLAST helps us compare the sequencing results of the plasmids...differences in the blastn programs, check out the BLAST Help webpage. Optimizing blastx searches Once you have... on our suggestions? Share your thoughts here to help other labs speed up their plasmid and cloning verification...

...recurring meeting amongst scientists that aims to help stay current on new and emerging work by collaboratively...unable to attend for scheduling conflicts. This helped us find a time that accommodates more people. The...(multiple choice/binary) and open questions is helpful to ensure acquisition of the essential data and...

... recruit parents from any background, it really helps if activities are run by parents who can answer ...scientist. I think that establishing this history helped demonstrate that a) I was a real person who used... it worked. I came up with an analogy that would help them grasp some of the key concepts (Fig. 2). One...

...productive and happy employees. The teambuilding culture helps us build relationships and thereby work better together.I...had in an orderly and timely fashion. 9. Ask for help My grandma used the old adage “many hands make light... lot of set up or cleanup, ask for volunteers to help out. Delegate- it will save you time and sanity!...

... covered! Here you will find some useful tips to help you make your plasmid construction with MoClo a ... are selecting for further assemblies. This will help confirm each part has ligated in the right order...in the right locations.   We hope these tips will help you in your MoClo quest, so good luck and happy ...

... can help you hop, skip, and jump between all kingdoms of life. A bit of careful design will help make...include viral oris to drive amplification, which helps increase cellular uptake, but this approach requires...

...These demo details help us see the problem from the user's perspective which in turn helps us decide how to...software design choices, resulting in software that helps our users focus on the fun stuff, accelerating research...

...looking to see if there are any resources that can help with the transition.” The lab-to-desk-job culture...formal training regime and the right set of tools to help you do your job as well as possible. It’s in their... is an amazing thing to be a part of and really helps me get behind a project or initiative.” If you think...

...GoogleScholar, Academia, ORCID, and MyScienceWork all help us share research. Open access journals also provide... by Scott Spicer at the University of Minnesota helps illustrate this: “In terms of total view count and...are some resources: ImpactStory, a website that helps researchers go beyond citation rates to measure ...

...At Future of Research, our volunteers have been helping to gather information for our FLSA and postdocs... us if you have information that you think would help our resource. In the medium term, it is unclear ...in advocating for and with junior scientists, to help them navigate the scientific enterprise and promote...

...pretty nicely displays her energetic, truthful and helpful style: Joanne also provided much more detailed...content for scientists in training is abundant, helpful, and sometimes excellent. Finally, as I said in... have questions? I am easy to reach and happy to help. Additional Resources on the Addgene Blog: 10 ...

...the functionality of your experimental system and help identify opportunities for troubleshooting or optimization...are control plasmids? Generally, control plasmids help to ensure that the observed phenomenon in your experiment...phenotype associated with the independent variable and helps ensure the phenotype isn’t singular or associated...

...offering advice on areas where you might need some help. Today in our transferable skills guide: Time Mangement... made small accomplishments each day that should help keep you content and give you the energy to finish...day, writing your to-do list the night before will help keep you focused. For a real-world example, let...

...step can involve multiple smaller steps that all help to ensure that antibodies can access their target...number of websites compiling antibody data that can help you compare antibodies from different companies,...Sharing your experience, positive or negative, will help make antibodies more effective and reliable in the...

...’s best efforts. We all have software tools that help us efficiently accomplish our daily goals. These...electronic lab notebooks into 9 questions that will help you start and define what exactly your lab needs... 21 CFR part 11, HIPAA? Answering this question helps you evaluate whether the ELN should have functionalities...

...plasmid, into which the GOI is inserted, and a Ti "helper" plasmid, which contains Vir genes necessary for...insertion into the plant genome. B) The Ti plasmid and helper are transformed into agrobacterium tumefaciens ...(yellow) are recognized by the Vir genes on the helper plasmid and used to insert the intervening region...

... set of plasmids together on a tight budget. To help popularize our kit resources,  we’re highlighting...selection markers built into all plasmids in the kit help decrease false positives observed in the cloning..." Small GTPases 7.2 (2016): 93-106. FREQ-Seq To help scientists track the frequencies of specific alleles...

... to quantify accurately, but can be especially helpful to generate more signal from low abundance targets...individual panels, then the multicolor overlay; this helps all viewers, especially if you have some complex...improved variations you could try. We hope this post helped you start planning for your next multiplex IF experiment...

...of known molecular weight in a separate lane can help confirm the size of the protein of interest. An ...  Visualizing your protein of interest with the help of secondary antibodies In order to visualize proteins...in your specific system. If you found this blog helpful be sure to check out Addgene’s blog for overviews...

... meet ups and happy hours that you can attend to help build relationships.  Reach out to a science policy...important question that you can explore. The answer will help you decide how to define the who, what, where, when...challenges you’ve overcome. This information may help you determine what parts of your current job you...

...rate of the vector you are using. Contact Addgene (help@addgene.org), or the supplier of your vector, to...before beginning large scale experiments. This will help to ensure the accuracy and reproducibility of the...Monosynaptic Tracing Success Depends Critically on Helper Virus Concentrations. Front Synaptic Neurosci 12...

...you need help putting your list together. Once you have your list of genes, Green Listed can help make the...CRISPR screen. Luckily, the Green Listed software can help you do just this, probably in less than a minute...