We narrowed to 808 results for: Eng

...bacterial infection, she shared that tweet with Jan Zheng, a UX designer that she knew. “We had actually met... met at a lindy hop swing dance event,” Zheng says (networking can happen anywhere!). The pair quickly...inception of Phage Directory ~1 year ago, Sacher and Zheng found that they could expand their role in the phage...because there is so much information out there,” Zheng says. In the past year, they found that phage information...potential for successful phage therapy, Sacher and Zheng also saw the need to archive phage reagents. If ...

... W., Li, Y., Feng, D., Ho, A., Nicholas, E., Hirokawa, K.E., Bohn, P., Joines, K.M., Peng, H., Hawrylycz...forming a neuronal loop which is activated and strengthened during memory formation and memory retrieval...modified and used as tools for tracing. With some engineering, RABV and HSV, but also others including pseudorabies...been found naturally or have been genetically engineered to have differences in their capsid proteins....S.K., Deverman, B.E., and Gradinaru, V. (2017). Engineered AAVs for efficient noninvasive gene delivery ...Koch, C., Bernard, A., Dang, C., Jones, A.R., and Zeng, H. (2014). A mesoscale connectome of the mouse ...

... Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high fidelity ligase—and...Optimization through high-throughput profiling New England Biolabs® (NEB) researchers recently published a...possible pairing resulted in a product of a different length, with products identifiable and quantifiable by...Greg Lohman is a biochemistry researcher at New England Biolabs, specializing in the enzymology of DNA ...G., et al. "Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase...17428930. PubMed Central PMCID: PMC1933048. 16. Cheng, Changming, et al. "Electrochemiluminescent assay...

...GeoCas9 started with an interesting question: why engineer a new Cas9 when you can just find one that naturally...naturally occurs? This is different than the engineered approach used to make eSpCas9 and SpCas9-HF1. By... PAM Sequence NGG CRAA (R = A or G) Spacer Length 20 nt 22 nt Temperature Range for Optimal Activity...prior research had shown that a cocaine esterase engineered to have increased thermostability also had a ...Read about CRISPR Methods for Bacterial Genome Engineering   Interested in using CRISPR/Cas9 RNPs? Check...

...competitive skill sets in a myriad of professions is to engage in extracurricular activities (EA). EAs can: Introduce...Introduce new perspectives in science and engineering Looking at your research from a broader viewpoint...expands your professional network. No brainer! Engaging audiences from across the hall, fields, and other...traditional academic setting and to shape and strengthen interests and skills. Although the word “extracurricular...potential EAs and which professional skill sets are strengthened. This short list highlights the ubiquity of ...

... effects on actin filament length (lower). d) Intensity and filament length traces for a different event...benzylguanine (BG) (Keppler et al., 2003). This engineered hAGT later became known as SNAP-tag. There is...cross-reactivity. Other strategies include using engineered ligases to attach a ligand to a peptide tag on... ligands are available from suppliers like New England Biolabs and Promega (though if you are comfortable..., M., Beaufils, F., & Johnsson, K. (2008). An Engineered Protein Tag for Multiprotein Labeling in Living...

.... This is like taking an engine out of the car – you can still use the engine as a power source, but now...experiments for FD-CF reactions can be fun and engaging for students. In our BioBits kit for example, ... a 4th year PhD student at MIT in Biological Engineering and is interested in developing synthetic biology... biology Use the pathways over time plasmids to engage students in functional genomics research Learn ...

... NIR light.  NIR wavelengths have better tissue penetration than shorter wavelengths, which makes it possible...will light up if excited with light of specific wavelength and also produce ROS - targeted cell ablation...iodine-modifications also shift MG-2I’s excitation wavelength to 666 nm, which is within the near-infrared ...non-specific ROS when exposed to YFP and RFP excitation wavelengths, which suggests that it could be used with these...

...by New England Biolabs, Inc. Many thanks to our guest blogger Lydia Morrison from New England Biolabs...contributed by guest blogger Lydia Morrison from New England Biolabs. What is DNA assembly? In the context of...the NEBuilder® HiFi DNA Assembly method from New England Biolabs® has minimal upfront requirements and allows...becomes hard to accurately execute. However, these challenges can be circumvented by generating individual ...

... that both the peak excitation wavelengths and peak emission wavelength of the two proteins should be ...with shorter wavelength red fluorescent proteins like TagRFP or mRuby2 than longer wavelength proteins like...the best protein for a given application is a challenge. To help keep you abreast of the latest fluorescent...

...recombineering-based MAGE (Multiplex Automated Genome Engineering) technology. pMA7CR_2.0 expresses lambda Red ...References 1. Maddalo, Danilo, et al. “In vivo engineering of oncogenic chromosomal rearrangements with ..., et al. “Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids.... Sakuma, Tetsushi, et al. “Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector...plasmids from this paper at Addgene. 5. Tsai, Shengdar Q., et al. “Dimeric CRISPR RNA-guided FokI nucleases...tools for efficient germline and somatic genome engineering in Drosophila.” Proceedings of the National Academy...

...algorithms, such as SIFT or PolyPhen to approach this challenge, using attributes of cancer variants such as evolutionary...to making cancer precision medicine a reality. Challenges for cancer variant characterization For many ...validation experiments. But, there have been two huge challenges preventing us from testing this hypothesis: ...Accelerator Plasmid Collections To overcome this first challenge, we’ve developed the Broad Institute Target Accelerator...cancer variants To overcome the second major challenge, we developed assays to test large numbers of ...

...components of EvolvR and its potential applications. Challenges of existing mutagenesis methods Several continuous...drawbacks with these approaches that makes it challenging for users to achieve targeted diversification...recombineering and Multiplex Automated Genome Engineering (MAGE) will always be limited by the size of ...investigate non-coding segments of the genome, or engineer microbes to perform tasks. EvolvR could also be...about other CRISPR methods for bacterial genome engineering   Learn more about lambda red recombineering ...

...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...all”. Each team member has unique motivations, strengths, and learning styles. For example, during my time...have led to frustration, micromanagement, and disengagement. By tailoring my leadership approach to each...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...team meeting to highlight their complementary strengths. By encouraging alignment on goals and clarifying...

...insoluble granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones...editing for psychrophilic systems as well as engineering and regulating psychrophilic recombinant proteins...community-centric science, our team has continuously engaged with a variety of stakeholders from industrial ...harmonious progress.     We’re always delighted to engage with potential collaborators and interested audiences...synthetic biology experiment Read our Genome Engineering Guide ...

...Sample6 still leverages the core engineering and scientific concepts to engineer bacteriophages and use those... As a biomedical engineer and scientist, I like to envision that my research will have a direct impact...himself from the idea of starting a company around engineered bacteriophages. He decided to quit his "safety...his co-founders raised $6 million. The biggest challenge during this time was learning to communicate effectively...

...are ~27kD engineered fusion proteins similar to a Fab fragment but created through engineering instead ...reduced-in-size options often have reduced binding strength, half-lives, and/or specificity as well. They’... repeat proteins, or DARPins, are genetically engineered small affinity proteins, around 14-15 kDs in ...produced in E. coli, and are both soluble and easily engineered.  Monobodies are ~10kD synthetic binding proteins...

... more about how you approach new challenges.     Know your strengths! Research experience presents ample...matter of applying that framework to the myriad challenges we encounter. As you spend time in a lab learning...yourself if you missed any important details. Or challenge yourself to think of more creative solutions. ...employer that you are well equipped to work through challenges from day one in your new position. Of course,...

...elegans Neuronal Gene Expression Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene...characterize the transcriptome. The first data release for CeNGEN is planned for August 15th, 2019. For more information...information about the aims and approach of CeNGEN, check out their publication.   3’UTRome The 3’UTRs of...popular! References Hammarlund, Marc, et al. "The CeNGEN Project: The Complete Gene Expression Map of an...

... sensor (GEX), where one wavelength changes with Ca while another wavelength is insensitive. Additional...requiring only a simple measurement. However, single wavelength measurements are more susceptible to artifacts...calcium reporter derived from troponin C. It is engineered with a reduced number of calcium binding sites...Cre-dependent expression. These sensors have also been re-engineered to measure changes in calcium specific to the...

...emphasizing the antibody’s specificity. Strength and accuracy The strength is based on binding specificity and...expression constructs to be conveniently modified and engineered.   Fig. 1: Antibody production methods... developed a criterion that includes accuracy, strength, and reproducibility. We test the antibodies on... negative control also determines the signal’s strength. Exposure time is the amount of time the sample...

...Here's what you'll find in this post: Epigenetic engineering with single-pot assembly Tracking cell-cell interactions...New items from the viral service Epigenetic engineering with single-pot assembly By Eric Perkins Though...epigenetic researchers custom design their own engineering plasmid tools in about a week. Taking advantage...H3K27me3), a linker between these modules (linker length can and should be varied as it can have important...in AAV1!  New ready-to-use AAV prep from Hongkui Zeng's lab are available for dual expression of cytoplasmic-tdTomato...

...Most things in biology are not so simple, and engineering complex cellular behaviors requires making complex...output, etc., which can support a larger cellular engineering goal, several of which are described below. Multigene...sometimes many times), and isolated afterwards. To engineer these production pathways, MoClo has been used... an array of distinct goals within metabolic engineering, synthetic product production, and gene editing...  References and Resources References Weber E, Engler C, Gruetzner R, Werner S, Marillonnet S. A modular...

...fluorescent signal can be affected by AT-rich DNA and the length of the amplicon and it has a higher rate of false...background noise, and is not affected by AT content or length of the amplicon. As the probes can have several...available, ddPCR may be a better option for more challenging cases.  Price point If budget is a concern...confounding by AT-rich DNA and by variation in amplicon length. Am J Trop Med Hyg. 2008 Dec;79(6):887-92. PMID...Abu Bakar, S., AbuBakar, S., Hollox, E. J., & Boon Peng, H. (2015). A comparison of assays for accurate ...

...infrared light to dissipate excess energy at wavelengths of low photosynthetic activity. This still allows...allows photosynthetically active wavelengths to reach zooxanthellae, the coral’s symbiotic dinoflagellates...Forster’s lab used codon optimization programs to engineer 14 chromoprotein sequences into E. coli BioBrick... Wistrand-Yuen, P., … Forster, A. C. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial...bacterial synthetic biology. Journal of Biological Engineering, 12(1). https://doi.org/10.1186/s13036-018-0100...

...Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering." Cell 157.6...not perform functional tests. The newest genome engineer sharing the stage with much-lauded CRISPR-Cas9...cleavage (1). Both the CRISPR-Cas9 and NgAgo genome engineering methods have their own pros and cons (2). The...of a sunny day in Edinburgh.   References 1. Gao, Feng, et al. "DNA-guided genome editing using the Natronobacterium...

...and offered engaging examples of how understanding genetics is so timely.” Genome engineering, and many ... an authority in this area. What is genome engineering? How does CRISPR work? This section was essentially...computers and the internet before it, genome engineering technology is moving along much faster than it...much in the making. One of the exciting, and challenging, aspects of talking to 8th graders was the broad...

...and projects. It is crucial to identify these strengths within yourself, and to demonstrate them to the...collaboration and fail to identify it as a personal strength until it is identified by another person. Similar...situations where you can learn more about possible strengths and weaknesses, and view everything as a learning...presents itself even outside of work, consider challenging yourself, take the opportunity, and discover ... strengths...

...to solve a problem. But how do you intentionally engage in a creative problem-solving process?  You may... needing to brainstorm with a team. One major challenge with these brainstorming sessions is avoiding ...you can, set aside time to prepare for future challenges. This doesn’t have to be time-consuming or even... keeping your science going throughout these challenging times.  Take a Break! If all else fails, one ... Originals how non-conformists move the world. Penguin Publishing Group. Day, F. (2019). Embrace your ...

...approximately 1000–1400 amino acids — which poses a challenge for efficient delivery into cells, particularly...Cas12a. Applications of TnpB One of the major challenges in genome editing is the efficient delivery of... enabling more precise and accessible genome engineering. More elaborate protocols are available in our...Additional Resources on the Addgene Blog CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9 RNA Interference... Additional Resources on addgene.org Genome Engineering Guide Plant Plasmids and Resources CRISPR Plasmids...

...soluble and insoluble proteins, which can be a challenge for classic antibody based affinity purification...for your proximity labeling experiment! APEX: engineered ascorbate peroxidase that can rapidly biotin ...available, the Alice Ting Lab demonstrated that engineered ascorbate peroxidase (APEX), used as a genetic...Ramanathan et al., 2018). To develop RapID, scientists engineered a mutant BirA* from B. subtilis, termed BASU....PMCID: PMC4296904. Martell, Jeffrey D., et al. "Engineered ascorbate peroxidase as a genetically encoded...

...BIOFAB Plasmid Set contain terminators of varying strengths centered within GFP/RFP bicistronic reporters ...required for eukaryotic systems and instead directly engages the ribosome for translational complex assembly...populations through time, Christopher Marx's lab has engineered and validated a new method called FREQ-Seq. This...proteins are composed of repeat segments that can be engineered to bind specific RNA bases. By swapping out these...2016): 895-899. Choudhury, Rajarshi, et al. "Engineering RNA endonucleases with customized sequence specificities...

...Regulating telomere length with TZAP. Listen to the segment on regulating telomere length with TZAP Hot Plasmid...tools for plants. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...🎄🎁🎅🤶🎄 pic.twitter.com/rDaEjxTNZX— Heng Chooi (@YitHengChooi) December 4, 2021   3rd Place: O! Beaker... Though 2021 had more than its fair share of challenges, the annual Deck the Lab Contest brought us smiles...Falcon Tube created by the team efforts of the Yit Heng Chooi Lab at the University of Western Australia...

...Cre-dependent conditional alleles, has remained a challenge. Enter Easi-CRISPR: a method that harnesses the...template. Although effective, ivTRT may be more challenging for sequences with complex secondary structure... systems (ttA or rtTA) are between 1 to 2 kb in length. There are plenty of Addgene plasmid options for...conditional alleles by CRISPR/Cas-mediated genome engineering." Cell 154 (2013):1370-9. DOI: 10.1016/j.cell... a robust method for in situ germline genome engineering using CRISPR nucleases. Genome Biol. 19 (2018...

...  We’ll look at examples of both natural and engineered repressible promoters. Chemically repressible...transactivator components of the system have been engineered and improved. Takara’s “2nd generation Tet-Off...system is a positive repressible promoter, and was engineered from the cmt and cym bacterial operons. A chimeric... and repress pADH1 activity. Researchers have engineered pADH1 variants that are less sensitive to ethanol...Introduction and expression of genes for metabolic engineering applications in Saccharomyces cerevisiae.” FEMS...

...become bored because I was not being mentally challenged. For these and other reasons, I started  looking...tuition fees had just gone up dramatically and, in England, you could not receive government loans to study...reason why I chose Australia was because it is an English speaking country with a strong economy and plenty...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like...

...PhD, achieving our career goals can be a real challenge. On top of that, in the midst of a rapidly changing...mentees with a broader, first-hand vision of the challenges and career opportunities that truly exist outside...was much more than that. I discovered that the challenges that I am facing in my career are common problems... that many others have faced yet, still, this challenge did not hinder them from advancing their careers...mentoring program extends its scope to the fields of Engineering, Economics, and Philology (in addition to Biomedicine...

...University of Malawi where he focused on agricultural engineering through biotech. After graduating with a masters...Kingdom's PhD projects - HIV inhibitors, biofuels, challenges in Malawi, and more 23:45-28:07 : How Seeding...Seeding Labs here. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had on the podcast...The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there. 13:22 - 22...of DNA and RNA.     Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...Alternatively, endogenous miRNA precursors can be engineered to created artificial miRNAs. The processed miRNA...frequently used. Although similar, amiRNAs are engineered from endogenous precursors of microRNAs found...specific than long hpRNAs, they must be individually engineered and empirically evaluated for their gene silencing...using a fluorescent output to monitor RNAi, we engineered a positive selection reporter whereby survival...Gong B-Q, Guo Y, Xu L, Zhang X-N, Li J-F (2018) Engineering Artificial MicroRNAs for Multiplex Gene Silencing...

...weighted by the position of the mismatch along the length of the gRNA given the stricter sequence requirement...cleavage assays to detect mutations triggered by engineered nucleases." G3: Genes| Genomes| Genetics 5.3 ...23934178. PubMed Central PMCID: PMC3782611. 8. Tsai, Shengdar Q., et al. "GUIDE-seq enables genome-wide profiling...detection of DNA double-stranded breaks induced by engineered nucleases." Nature biotechnology 33.2 (2015):...23503052. PubMed Central PMCID: PMC3651036. 15. Tsai, Shengdar Q., and J. Keith Joung. "Defining and improving...

...and creativity in the face of funding challenges. By strengthening our commitment to collaboration and ...and increased tariffs present an unprecedented challenge to the scientific research ecosystem, not just...years to come. We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to ...

...repair, stem cells, gene therapy, and tissue engineering. How the multidisciplinary nature of the Center... Chapter 6: Near-term possibilities for using engineered cells in therapy. The lungs are difficult, but...Boston Medical Center. Special thanks to our sound engineer/producer, Eddy Page! Edward B. Page is a Digital...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...

...2013. Since that point, it has become the most engineered Cas enzyme, with adaptations for increased fidelity...target and degrade select RNA transcripts, or be engineered to conduct direct RNA editing. There are four... L. A., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/CAS systems. Science, 339(6121),...Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D., Haft, D. H., Horvath, P., Moineau, S., Mojica... J., Chong, S., Makarova, K. S., Koonin, E. V., Cheng, D. R., & Scott, D. A. (2018). Functionally diverse...

...CASTs quickly became popular bacterial genome engineering tools, with early reports of 40%–60% efficient...catalyzes DNA insertion, along with both evolved and engineered adjustments to the other components. It drove... & Sternberg, S. H. (2024). Bacterial genome engineering using CRISPR-associated transposases. Nature ...the Addgene blog INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Degrading DNA with ...

...simply with the presence or absence of the correct wavelength of light. This response is immediate, on the ...activation to behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics...Optogenetics also requires that light of the correct wavelength reach receptors in the brain. One way to address...can be simply introduced by injection. The main challenge in using ligands is ensuring the agonist is able...

...should possibly take a composition class in the English Department. He suggested that I sign up for the...the SWPI online course because a course in the English department wouldn’t have trained me in the scientific...not prose; it is to be straight to the point. 3. Engage the reader Scientists forget that the reader is...has to introduce the reader to the manuscript and engage them in the material. So the manuscript should ...

...in the past year. Cambridge Biological Society, England Since 1996, Cambridge Biosoc’s goal has been to... up to professors. Oxford Biological Society, England The Biological Society based at the University ...networking events. Imperial Synthetic Biology Society, England The Synthetic Biology Society is a new society ...incredibly glad to see these biological societies engaging with young scholars. We believe it’s very important...

...the full length plasmid. If you find that your bacterial cultures contain a mix of full length plasmid ...will also outgrow bacteria that contain the full length plasmid.   Figure 1: The full lentiviral...like DH5-alpha are recA-, some strains have been engineered to further reduce recombination. Recombinase-...