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We narrowed to 929 results for: KIN

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  1. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...to the meeting, and its history of code making and code breaking is wonderfully analogous to genetic circuits...our baffled flight attendant called them, "long, skinny things") and generally exuding a very-tired-but-very-excited...as an undergraduate, I never fooled myself into thinking I was an engineer. The talks we heard Monday night...discussed in the book). These synthetic biologists are taking the "genetic code" to a new level, changing it ...years. I kept busy for the whole poster session, talking to people from every conceivable background at ... Eric J. Perkins...

  2. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    ...Joanne's Reddit AMA  Few of us spend much time thinking about conserving resources in the labs. Disposable...Founder of Seeding Labs, tells the story about working as a student in a rice lab in the Ivory Coast where...very environmentally conscious friends who had me thinking about energy conservation even before it was a...accessed blogs on BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, ...founder, Melina Fan, recently shared our protocol for making competent cells to save time and money. My other... portion of the gel.  What a waste?  I started looking for smaller and smaller gel apparatus until I settled... hilarious.  Birth of the baby gel box Finally, taking conservation to new levels, I was bothered by the...

  3. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

    Type
    Blog Post
    ...different kinds of grants that you can apply for. If you are in a lab, your PI is likely working on a number..., we posted these 25 great tips for scientists working at home, and you will still find this useful. Now...been doing this for almost 6 months, you may be looking for more ways to make sure you are moving forward...Addgene have gone virtual!   2. Practice public speaking For almost any science career, there will be a...good progress on both building relationships and thinking about future career options. 5. Write a grant ...Science If you have a method that you've been working on, let us know in the comments below! Additional...

  4. The Strength of Story Telling

    Type
    Blog Post
    ...I’m working with a talented journalist to shine light on living as a scientist in Beijing, talking to ...to people about taking beautiful photographs of their studies, reporting on amazing women in science, or... or asking someone how and why they left academia. Humans are designed to pay attention and enjoy anecdotal...We build narratives around the self, too — when talking to friends or dates or colleagues we tell tales... a solid point to kick off at. Whatever you’re working on, start wherever it makes sense to start and ... overnight. The only way to improve is to keep working on telling a story again and again and again until...

  5. Hot Plasmids: Spring 2025

    Type
    Blog Post
    .... Both have significantly improved brightness, kinetics, and sensitivity. iGluSnFR4f is well-suited for...larger signal-to-noise ratios in two-photon imaging, making it ideal for video recordings of large populations...generation and stable cell line creation (Figure 2). Looking for more? Explore our empty and EGFP retroviral...endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to insert the payload sequence ...Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc transposon protein...Cas9 nickase to improve target recruitment and nicking, producing STITCHR. STITCHR can make a wide variety... on other proximity ligation tools with better kinetics and less background labeling. By fusing miniTurboID...

  6. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...genome is 1 in every 256 base-pairs of DNA sequence, making it very useful for genetic engineering approaches...transgenic cell lines. The traditional method of making stable cell lines is to transfect, select, and ...negative impacts on fitness. One consideration when making a transgenic cell line with the piggyBac™ system...However, several challenges still remain, such as making gene edits completely seamless (i.e., so no other...piggyBac™ transposase (PBx), which contains mutations making it competent for excision, but defective for integration...containing a negative selection marker such as thymidine kinase, a researcher may subsequently kill off any cells...target sequence. This allows researchers to avoid making unnecessary base pair changes as would be the case...

  7. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...everything click in my head and left me thinking: I wish I had this kind of experience earlier! After all,...biology in high school, it wasn’t until I started working in my first molecular biology lab in college that...more and more molecular biology-based activities making their way into high school classrooms, like gel... on freeze-dried, cell-free (FD-CF) components. Making proteins outside of a cell - cell free extracts...the protein in a cell-free manner. This is like taking an engine out of the car – you can still use the...cost-effective, as each one is as cheap as 10 cents, making activities designed with FD-CF reactions affordable...

  8. Learning Technical Skills at a Lab Central Workshop

    Type
    Blog Post
    ...useful to anyone working in a lab.   Participating in a workshop at lab central We’re working on a project...of experimental services and expertise without breaking their bank accounts. When I heard about how LabCentral...partner for the day was Julie from Novartis who was working with cell culture and who was extremely helpful... now know what to expect at a workshop and what kinds of questions to ask myself when I am bombarded with...new without spending a lot of time developing and making space for the techniques ourselves. As you probably...should give similar workshops a try if you’re ever looking to try out a new technique or reagent! Racheal...

  9. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...Review dropped a bombshell report. A scientist working in China was using CRISPR/Cas9 in an attempt to...can happen. We just have to look to the United Kingdom’s Nuffield Council. While the Nuffield Council has...tasked two separate non-governmental groups with seeking “public views on emerging IVF techniques designed...addition to thorough ethical considerations, in making sure that the research has been adequately proven...experiment on humans, and they are not remembered kindly. However, despite many headlines questioning if...otherwise, the scientific process does seem to be working. I would argue that one of the takeaways from He...whom are named Charlotte, all of whom are still working on theirpenmanship. Additional resources on the...

  10. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Addgene, who will be taking over the distribution to expand the network of scientists working on this even more...throw a therapeutic monkeywrench into the virus’ hijacking of human cells. Even better, more than half the...the Krogan lab's Addgene page to get notified!) Attacking coronavirus, one FedEx package at a time Since...interest, because it means more scientists will be working on this problem more quickly. Figure 2: Getting...headache receiving constant email requests and tracking their fulfillment manually in a shared spreadsheet...interested in facilitating collaborative science, and making it accessible to all people, not just scientists...

  11. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...distanced work more creative. An example could be making lab meetings more interactive and creating space...interaction and brainstorming. This could be achieved by asking each presenter to come up with a scientific question...: “Science is increasingly done in large teams, making it more likely that papers will be written by several...taste of that casual kitchen chatter, ultimately sparking new ideas. Social no-science-y meetings Set up...probably lost during these weird times of shift working and social distancing. The take home message is...are endless. Let’s adapt and embrace new ways of working, reconnect as human beings and colleagues, eventually...confidence to launch new and diverse careers by taking part in career panels, and volunteering for scientific...

  12. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...projects range from hacking probiotics to filming educational lab videos, while some are looking to develop new...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...independent Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate in the... gene drive paper was very popular. People kept asking if we were teaching classes at the lab, so in December...That’s right, we have non-traditional scientists working in our laboratory! Don’t worry though, we make ...transformation guru and instructor for our intro to biohacking courses. Our members pursue a diverse range of...

  13. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ...for tagging experiments and it’s already looking like it’s making a big difference. We can now look at many... an adjunct faculty member) have together been working toward this goal for years as members of the ENCyclopedia...actually use this for tagging any protein in all kinds of different assays, not just transcription factors...variations on the plasmids we’ve developed. We’re working on that, but the system is so flexible. Other investigators...Fragment from Integrated DNA technologies. Generally speaking, anyone using CRISPR/Cas9 in the lab already should...Thank you to Professors Mendenhall and Myers for speaking with us! Find the Plasmids: Plasmids for protein...

  14. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    ...interested in the same phosphate of your favorite kinase is a good start. Check out Joanne's Reddit AMA ...general intro until you know if the person you are talking to is really in your direct area.  Review the conference...see when your connections post. Stay in touch by liking, commenting and re-sharing their posts. LinkedIn...colleague. Recommended Reading & More: Scientist Networking: What is an Informational Interview? Crash Course...Julio Peironcely on the Thesis Whisperer "Not Networking - Building Relationships for Success" webinar... improving your networking and soft skills ... scientist networking...

  15. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    ...continued working for Palladian Partners remotely, but started looking for a new job because “it kinda sucked...social media manager. “I usually start out my days looking through our Twitter feeds and dealing with social...difficult pieces: “It’s probably the best.” Besides working through the tough assignments, the skills that ...include: “Listening. Evaluating evidence. Critical thinking. Being able to learn things quickly. Story telling...government, so it was a really good environment to be looking for a job.” Thanks to Susan for not only being ...

  16. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...Coaching can be an excellent tactic for scientists making a career shift or who wish to improve their current... responsibility seriously. As I make my rounds speaking at research institutions and at scientific conferences...take some research.  It helps to spend some time thinking about what your goals are for the coaching relationship...out of academia (because so many scientists are making this transition). Another coaching group is Still...Humphrey from Jobs on Toast is based in the United Kingdom and scientists there would be lucky to work with...with him.  He is also available for speaking engagements as are many of the coaches I have mentioned. I...Entropia Consulting works remotely, will travel for speaking engagements and has many areas of specialty from...

  17. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...localization, turnover, and trafficking, as well as organelle trafficking and dynamics (fission, fusion...light is more phototoxic to cells, most scientists working on in vivo imaging models perfer the PA-RFPs over...from a dark state to a bright fluorescent state (“kindling”), and from a bright state to a dark non-fluorescent...fluorescence usually means more photons are being emitted, making it easier to capture a clear image above background...for whole cell imaging and studying organelle trafficking. Monomeric PA-FPs are usually preferred for studying...PMC2901231. 3.Verkhusha, Vladislav V., and Alexander Sorkin. "Conversion of the monomeric red fluorescent protein...Photoactivatable GFP tagging cassettes for protein‐tracking studies in the budding yeast Saccharomyces cerevisiae...

  18. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ...governing policies are developed primarily by wishful thinking. The March for Science is about fighting scientific...postdoctoral researcher at Harvard Medical School working on human artificial chromosomes. Montreal, QC, ...scientists, we work tirelessly in the lab where the groundbreaking research happens, sometimes forgetting how ... I was scared. I worried that, because we were speaking out against legislators and because the march ...AggleDetector on Twitter) is a graduate student at Johns Hopkins University, where he is trying turn a few exoplanets...it takes to complete your resume, without ever thinking that research scientist would be a career that...NIH budget were proposed. I spent a lot of time asking why. As scientists we spend years being taught ...

  19. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...with different kinetic parameters. There are three GCaMP types based on their kinetics: GCaMPf (fast) ...bones and teeth. It is an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator...sensor to use for your studies, you can start by asking ‘What is the biological phenomena I want to observe... a sensor that is tuned to your parameters.   Kinetics How fast is the process you would like to monitor...jGCaMP7 and jGCaMP8 are also available with improved kinetic and sensitivity parameters. Sensitivity and Localization...to variance in sensor expression if you aren’t working with a stably integrated biosensor. Ratiometric...

  20. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand is also important for ...delayed and takes on the order of minutes. Studies looking at immediate effects of specific neuron firing ...blood-brain barrier.  Another consideration when picking ligands for chemogenetic studies is whether or ... are viable for translation to human patients. Picking receptors The receptor you choose is largely dependent... you to directly manipulate ion channels. When working with PSAM receptors, your impact on the cell is...Liss B, Kätzel D, Nissen W, Pekcec A (2019) Pharmacokinetic and pharmacodynamic actions of clozapine-N-...

  21. Tips for Technical Support Calls

    Type
    Blog Post
    ...pretty good about sticking to that. If you email us at noon and then call back at 1PM asking why you haven...Tweeting and texting, actually writing an e-mail or making a phone call is a big commitment when you’re busy...doctoral dissertations. Streamline the question asking process Having thought through the above, don’t...soon as possible!” and I always begin my responses asking for more specifics. We can help you faster if we...how long it will take to get an answer, consider asking to avoid unrealistic expectations. And finally... Eric J. Perkins...

  22. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...the intensity of fluorescence signal and enables tracking of single molecules within living cells without...roles in many cellular functions, from endosomal trafficking to signaling pathways to the cell cycle. Good...different chromosomes. The advantage of having this kind of system that works in live cells is that one can...used with your own reporters to decipher their trafficking routes. This collection is now available at Addgene...indicators should possess fast voltage sensing kinetics and have no spectral overlap with the optogenetic...and an endoplasmic reticulum export motif and trafficking sequence for improved targeting to the plasma...CheRiff, exhibits high light sensitivity and fast kinetics. By combining both the voltage indicator, QuasAr...

  23. Technical Design of a Western Blot

    Type
    Blog Post
    ...hardware sold by the manufacturer. Blocking solutions Your blocking options come in two categories: protein-based...make when designing a western blot.  Psst! Looking for a western blot protocol? Check out Addgene'...antibodies are relatively cheap and easy to source, making it easy to switch from one readout method to another...re running native (non-reduced) proteins or are looking to preserve specific protein modifications, we ...single-percentage gel, which is cheap and useful when you’re looking at proteins all roughly the same size, or a slightly...information. Many antibodies will have a recommended blocking buffer and a recommended concentration. That makes...greater dynamic range than chemiluminescence enzymes, making it easier to accurately visualize proteins of wildly...

  24. Transferable Skills Guide: Managing a Team

    Type
    Blog Post
    ...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...undergrads back in grad school. Both were hard-working scientists-in-training who got the job done, but...left him/her with small tasks and s/he was always asking for more work (this is a big hint that you are ...on what you should be doing : writing a grant, thinking about the next project, reaching out to other ...really helpful. “What is the best/worst part of working with X?”  is going to get you what you really want...grateful for the feedback, but at least you can start making changes for the best. Positive feedback is equally...Scientists Series What Makes a Good Mentor? Tips for Working in Industry and Success in Management Resources...

  25. Transferable Skills: Negotiation

    Type
    Blog Post
    ...researchers learn many transferable skills while working in the lab and by offering advice on areas where... jobs needed to be done (Who is responsible for making the running buffer this week?). Scientists negotiate...approached it as a creative problem-solving exercise? Asking questions like the below can help you understand...you are in the middle of a frustrating situation. Asking for opinions from a neutral third party can be ...offer conflicts with a pre-planned vacation I’m looking forward to. How can I negotiate my starting date... a tenured professor or signing contracts when working for a business development team, negotiation is...strengthen your relationships with others who know that working with you will benefit them too. Ed's note: Salary...

  26. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...the conformational dynamics of the motor protein kinesin-1, the Twelvetrees Lab took advantage of the self-labeling...and more photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques...deposited an array of constructs for tagging the kinesins KIF5A and KIF5B as well as backbones for generating... will be useful not only for future studies of kinesins but for many other proteins of interest too! Find....A.S., Craggs, T.D., & Twelvetrees A.E. (2024) Kinesin-1 is highly flexible and adopts an open conformation...voltage imaging. A) ASAP5 selected for its improved kinetics and responsivity in screening ASAP3 variants. ...different illumination regimes with fast activation kinetics and high responsivity near the resting membrane...

  27. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...structural biology, Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution...model. Until then, you can only wonder what I am talking about. How do researchers use physical models of... over and over in their hands. They then start talking to themselves as they compare the features displayed...magical teaching moments. The models function as thinking tools – eliciting questions like “what does this...investigated in their labs. Soon after we began working with educators in our Center for BioMolecular Modeling...complementary DNA strand. And this is why we love working with researchers on a model. The little knowledge...Jennifer Doudna’s new book A Crack in Creation. Making models more useful through flexibility It is common...

  28. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...of His-tagged human kinases in bactera. Listen to Our Segment on The Human Kinase Domain Constructs Kit...We’re breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward ...CRISPR X from Michael Bassik's Lab - A new tool for making diverse, localized point mutations. Listen to Our...Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's Lab -...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...

  29. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    ...scientific discovery and career development. They are working with limited resources but limitless potential....by facilitating connections to mentorship and networking opportunities, publishing assistance, and more...international scientific community and are always taking steps to increase plasmid distribution and disseminate...distribution As you can see from the chart below, we are making great progress toward spreading the Addgene community...countries. As part of these efforts, we will be working with Seeding Labs to award plasmids to research...well as shine a light on some of the great minds working in developing countries. As the world continues...

  30. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    ...been extremely helpful for scientists that are looking for more detailed information about this new technology...topics you want to learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular...additional questions. We’re happy to see scientists making their own cells to save money and create the tools...feature in the coming year. Finally, we are always looking for guest bloggers who want to share their research...Replication By Kendall Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids...Marcy Patrick Will You Be My Mentor? Finding and Asking for Mentoring Support By Joanne Kamens Plasmids...

  31. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...Mortensen, a PhD candidate at Northeastern University. Working with plants doesn’t always have to be a time-consuming...recently overcome this problem in C. roseus by working with young seedlings and optimizing several other...transient leaf transformation: Check if there are working protocols available for your species This will ... your tissue. Run a control with Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like.... Successful infiltration can often be seen by sinking of the tissue (see Figure 3). This method is probably...GV3101(pMP90), which works well for us. Use a well working vector system This allows you to quickly assemble... you started. Once you have established a well working protocol in your lab, it can become a strong tool...

  32. How to Be an Excellent Trainee

    Type
    Blog Post
    ... you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee possible? ...articles on building successful mentor relationships, asking for mentor support, and choosing your mentor. What...And I mean everything! The bare minimum should be taking notes on general protocol steps and other tips ...the moment….but you’ll probably forget and end up asking your mentor later if you don’t write it down. And... to your mentor. Pro tip! Consider physically walking through the protocol, by going to each space in...tell a trainee is understanding and things are ’clicking’ for them. It’s often easiest to train individuals..., What Now? Will You Be My Mentor? Finding and Asking for Mentoring Support   ...

  33. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    ...the clock for others to dive in. The real cost of making data accessible A more reasonable concern with ... the scientific community is thus not averse to making data available, but there remains concern about... by others. Other new data initiatives are also taking root. Nature Publishing Group recently launched...efficient searching and extraction of information. Looking a little further ahead, journals may move towards...Instead of providing a “dead end” figure or table, clicking on the published chart could reveal the underlying...reader as possible. Rather than adding to demands, making effective data sharing the norm should open up ...Canada where he studies protein-serine/threonine kinases implicated in human diseases. His spare time is...

  34. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...of naked DNA. Other investigators who had been working to develop high efficiency gene targeting approaches...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... CRISPR-based approaches for the generation of knockins via homologous recombination with a homologous...application - the creation of sequence-specific knockins - for which the AAV approach is most well suited...suited, since it routinely generates knockin efficiencies of 1-40% with G418-selected clones. However, there...approach requires G418 selection of infected clones, making it unsuitable for most in vivo applications.  Third... CRISPR-based approaches for the generation of knockins, it is not always ideal. Because of these limitations...

  35. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment...minimizes sample loss while also supporting imaging, making it easy to identify dozens or hundreds of particles...molar ratio) with 10 minutes of glutaraldehyde crosslinking: representative micrograph (C), particle orientation...could be overcome with methods such as chemical crosslinking or tilted-stage imaging. A truncated form of...compatible with the buffer additive MgCl2 and the crosslinking agent glutaraldehyde. Adding RvLEAMshort to ...j.cell.2021.03.025.    Recombinant antibodies for marking cell types in the nervous system By Ashley Waldron...filament expressed in astrocytes throughout the brain, making it a popular target for labeling these cells. Check...

  36. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ... pathway. Those can include cytokines and chemokines; enzymes like kinases, phosphatases, and ubiquitin...false positive signal. Dump gating When you are working with heterogeneous cell mixes, like bulk lympho... buffer solutions. Fixing causes protein cross-linking, killing the cell but preserving the cellular state...gating uses the same color for different markers, making it easy to gate on the negative cell population...investigating negative regulators of T cells and cytokine signaling in tumor immunity. When he's not in ...

  37. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...in vitro kinase reactions. Often, however, researchers lack knowledge of the regulatory kinase, access ...access to active kinase for the reaction, or have difficulties with kinase specificity in vitro. The establishment...phosphorylation sites. If knowledge of a candidate regulatory kinase is known, it’s possible to generate non-phosphorylated...phosphoserine into recombinant proteins, such as the kinase MEK1 (5). MEK1 is inactive without requisite dual...create a pSer/phosphomimetic dual mutant) elevated kinase activity over 70-fold over dual phosphomimetic ...

  38. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...every day, sharing ideas for the company, but also talking about sports, movies, and just about anything. ...cite lunch as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can...Chef-style competitions Every few months, we’ll have a cooking competition featuring a selected ingredient. Anyone...cook-off last week, and the featured ingredient was pumpkin. Everyone gets to vote for their favorites, and...the stiff competition or says something about my cooking.   This is a creative (and delicious) team building...work better and keeps us smiling even when we’re packing the 400th plasmid that day.   As we head into Thanksgiving...

  39. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...Does this sound familiar? Well, when it comes to working with Addgene, we certainly hope not! One of our...email. You need help right at the moment you’re looking for it, or you may just prefer to troubleshoot ...icon, search for answers, and choose a result. By clicking on the result links themselves right from within...ll navigate to articles answering your questions making it easier than ever to get the support you need...some reason you can’t find the answer that you’re looking for, or you just have a general question or concern...valuable feedback. This is Addgene after all, and making your research easier always comes first. Resources...

  40. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...have an opportunity to improve my networking with mentors who are working in USA and Europe, something that...who migrated from their country some years ago looking for their career improvement) and bright students...world in flux. The professional development and networking resources offered by programs like IMP USA-Europe...Institute of Technology, Boston University, Johns Hopkins University, the Salk Institute, and the Karolinska...and contacts with Spanish laboratories to start working on projects together. List of discussion topics...Addgene Read Other Career Blog Posts Read the Networking for Scientists Series Read our Blog Post about...

  41. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    ...fused to calmodulin (CaM) and the chicken myosin kinase calmodulin binding region RS20. When calcium is...place for the biosensor to be expressed. Are you looking at an intracellular or extracellular molecule? ...extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the other end ...with your lab and experimental setup? Consider what kind of instrumentation you have available, and the conditions...and multi-color imaging experiments. Finally, what kind of system will you use to detect your fluorescent...method depends on what type of information you are looking for. Below we have summarized some of the common...

  42. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    ...its value. My master’s strengthened my critical thinking and problem solving abilities, increased my technical...writer,” among others. Don’t discount job postings looking for PhD applicants since many will also accept ...people describe their actual work. To do this, try looking at LinkedIn profiles of people who have masters...Is their career path one you could see yourself taking? If so, consider setting up an informational interview...degree prepared them for their career.  2. Are you looking for career advancement and growth? A master’s is...project.  3. Do you prefer being a generalist? Working on a variety of projects and in different fields...

  43. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...equipment acquisition, public speaking and personnel management?  If you are thinking about a non-academic path...advisors learning how to be a scientist. It is shocking how little pre-work most PhD students and postdocs...learn how to be a scientist. Your PI should be the kind of mentor that will teach you how to: interpret ...with the potential advisor and don’t be shy about asking hard questions. Talk to as many lab members as ... time with everyone. Additionally, if you are looking for more resources to help you choose a good mentor...

  44. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...Welcome to our Summer SciComm Series, where we’ll be talking about science communication, best practices in ...science communication research.   This summer, we’re making time to talk about my favorite thing - science ...but the foundation of all good communication is making sure everyone’s on the same page. So for our first... endless.    Activity Example audience  Talking about your career as a scientist underrepresented...enjoy listening to podcasts A series of Tweets breaking down a new publication that could advance treatments...Communication - 9 Ways to Get Your Foot in the Door Taking Science to Elementary and Middle Schools   Image...

  45. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...cell membrane.  Picking the Right Antibody  It can get even more complex if you’re looking for proteins ...keep epitope availability in mind whenever you're working with antibodies.) Conformation changes usually ...Processes  If you’re feeling relieved that you’re working with native proteins instead of denatured ones ...are interacting with one another, or if you’re looking for a protein of interest while it’s in a transition...validated for ELISAs, it has a good chance of also working for a Western because they both typically use denatured...

  46. Donations from Addgene to Yield Answers for Rare Disease Researchers

    Type
    Blog Post
    ... sponsors a global competition for researchers working on rare diseases, offering them the chance to win...Claudio Aguilar (right) at Purdue University and is working to understand Lowe Syndrome, a devastating genetic...great for us,” Wang said. “We were planning on and looking at a bunch of different strategies with CRISPR-...hearing loss and changes in coloring of the hair, skin, and eyes. The researchers found that seven patients... budget is approximately 20,000 USD and we are working on several genetic diseases at the same time. The...studies.” Thank you to all the BeHeard Awardees for speaking with us! Additional Resources Find out more ...

  47. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ... to email strangers asking to meet. I have cold emailed hundreds of people asking for meetings to discuss... carry a heavy price tag, especially if you're working on something other than software.  If you are a... your entire career in science, you are likely lacking the necessary business background and/or connections... Start with your network You've spent your life making connections and finding mentors -- well, it's time...discuss each project and business decision before making it, to have an internal review system.  On top ...

  48. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ... and high resolution functional tracking of T lymphocytes, too, making them a versatile tool for application...Max Planck Institute for Neurobiology has been working on genetically encoded indicators of calcium and...has a particular advantage for observing events taking place inside cells that are on the move, such as...element-binding protein (CREB) in living cells. “Now we are making this really good,” Griesbeck said. “We know CREB...genetically encoded magnesium sensor, the first of its kind. “I hope by depositing at Addgene, people will start...

  49. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

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    Blog Post
    ... optogenetic tools in a deep part of the brain, making it sensitive to light. When they illuminated this...patients? Over a quarter of a million people have some kind of neural implant already. If we can use light to...maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other conditions.... emotions, to see how a monkey changes decision making, to cure a human patient. We can't do it all by...you have any special tips or tricks for someone looking to try these optogenetic tools out for the first...always tell people, 'Be driven by your science.' Looking for Optogenetics Plasmids? Plasmids for Optogenetics...

  50. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post
    ...gene edit. I wanted to inactivate the gene BRAF (a kinase implicated in several human cancers) in A549 cells...Vinculin is a loading control. 3. In terms of making the monoclonal line: I had never done it before...found here. Figure 3: A surprise for the author. Checking Cas9 expression Overview: By Western blotting ...difficult part of the assay was designing primers flanking my edits in genomic DNA and doing PCR on genomic...agarose to get sharper bands. Conclusion Overall, making a human cell line with a gene knockout was straightforward...who you are, you could quickly be on your way to making new scientific observations. References 1. Rago...C, Vogelstein B, Bunz F. Genetic knockouts and knockins in human somatic cells. Nature Protocols 2007;...
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