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  1. Depositor Week 2020 is Happening Now. Join us!

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    Blog Post
    ...! Thank you depositors! In fact, this year, Addgene depositors quickly stepped up to make COVID-19 ... 2,300 COVID-19 related plasmids currently in Addgene’s repository which have been requested about 1,500...whether you are a depositor or not. We will have Addgene trivia (with prizes!), polls, and shout outs, and... and we’ll be featuring the work of the Addgene community. Perhaps you’ll find plasmids and new tools ...We’ll also take a look back on some fascinating Addgene history that you might not have known about. Here's...Thursday: Throwback Thursday (Blue Flame awards and Addgene history) Friday: Wrap up! To show the magnitude...2021. Check out the infographic below for some Addgene depositor fun facts for a quick way to grasp the...

  2. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post
    ...with detailed protocols, go to www.addgene.org/crispr/tagging. Addgene: What motivated you to adapt CRISPR...tagging system, which they’ve now made available at Addgene. For more details on the plasmids that are currently...can now look at many more transcription factors. Addgene: How does the system work? Mendenhall: The system...antibodies. The results look almost identical. Addgene: What advantages does your system have in this ...faster. It’s also easy to run things in parallel. Addgene: Besides being fast and efficient, what other challenges... hundred different versions of this out there. Addgene: Why was it important to you to make these plasmids...waiting and hands on labor. That’s a huge deal. With Addgene, now these can be available to anyone for a modest...

  3. Tips for Technical Support Calls

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    Blog Post
    ...Resources on the Addgene Blog Learn About the Addgene Help Center Get Help with the Addgene Deposit Process...emails in my six years as a senior scientist at Addgene. Having spent that long in customer service, I'...Though my experience is based solely on my time at Addgene, I’m confident that these tips and tricks will ...another great source of information. At least at Addgene, the questions in our FAQ section are really, truly...not realize it, all of the reagents available at Addgene come from researchers, and the vast majority of...happens more frequently than you might think. The Addgene scientists answering the phone are smart, but they...much information as possible about the issue. For Addgene support, make sure to mention the plasmid catalog...

  4. Antibodies 101 Series on TikTok

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    Blog Post
    ...animation is becoming a TikTok series! Follow @addgene on TikTok for bite-sized portions of antibody concepts...an antibody?" or "what is an IgE isotype?"  @addgene Ready to learn about antibodies? Our new antibodies...molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations regularly ... all of our videos will also be shared on our @addgene Instagram account, or you can watch the full animation...on our YouTube channel.  More resources on the Addgene blog Antibodies 101: Introduction to Antibodies...

  5. Viral Vectors 101 eBook 2nd Edition

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    Blog Post
    ...Resources on addgene.org  Addgene's Viral Vectors Guide Addgene's Chemogenetics Guide Addgene's Optogenetics... our collection, on the Addgene eBook webpage.  More resources on the Addgene blog CRISPR 101 eBook en...

  6. Four Factors that Differentiate the Stem Cell Field

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    Blog Post
    ...What an honor for Addgene to be a part of that!  Additional Resources on the Addgene Blog Learn about...called OSKM factors). As the Yamanaka lab's primary Addgene contact for many years, I've been looking forward...example of the fast movement in this field.  As an Addgene scientist, I'm exposed to many scientific communities...quality control process alone takes nearly a year. Addgene depositor Juan Carlos Izpsua Belmonte said it took...poster presenters, it was gratifying to see that Addgene has ensured that plasmid acquisition is not a rate-limiting...community with their research, I think I can speak for Addgene when I say that I'm glad we're so fast and affordable...some of the scientists I've already mentioned, Addgene depositors Marius Wernig, Kristin Baldwin, Kathrin...

  7. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post
    ...Resources on addgene.org  Download Addgene's Viral Vectors 101 e-book Additional Resources on the Addgene Blog... by Addgene in 2021 asking scientists what data would improve our AAV Data Hub.   The Addgene Data...Share AAV Data with Addgene's New AAV Data HubCelebrate Open Data Day with Addgene’s AAV Data Hub!Data ...neurons transduced by each serotype. Working at Addgene has shown me this was far from an isolated case...vector performance. Open science initiatives, like Addgene’s Data Hub, provide an avenue to more easily solve...desirable  In a survey we conducted to improve our Addgene Data Hub, 76% of respondents indicated they wanted...the public where it can be utilized by others. Addgene’s AAV Data Hub is one resource where this type of...

  8. CRISPR 101 eBook...en español

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    Blog Post
    ...disponible en español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar...emocionados! Después de recibir la traducción, Addgene contrató a una traductora científica profesional...Descargar CRISPR 101: Una herramienta de edición Addgene tiene una gran audiencia internacional, incluido...available in Spanish!  This translation was gifted to Addgene by Daniel Mejías Sánchez, and we could not be more...more excited! After receiving the translation, Addgene contracted with a professional scientific translator...Download CRISPR 101: Una herramienta de edición  Addgene has a large international audience, including working...

  9. The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years

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    Blog Post
    ...Addgene was founded 10 years ago today. In that time, Addgene has shipped over 350,000 individual plasmids...about Addgene's 10 years of plasmid sharing? Check out this infographic created by our own Addgenie, Laura...Top 10 plasmid technologies distributed through Addgene over our first 10 years. 10 most distributed plasmid...it's not actually a technology, but pSV-PGC1 was Addgene's first distributed plasmid and was created in the...repository. 100,000 Plasmids Shipped! - By 2010, Addgene had hit our stride. We shipped our 100,000th plasmid...of the year in our collection. CRISPR/Cas9 - At Addgene, 2013 will be known as the "Year of the CRISPR....challenging and rewarding journey since starting Addgene. Thank you for inspiring us with your breakthrough...

  10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post
    ...Foundation's Linda Kahl explains the Addgene collaboration this way: "Addgene is one of the leaders in distributing...to have Addgene as a professional public-benefit partner who shares our core values." Addgene spoke with...bioeconomy. Addgene: What do these BIOFAB and BIL gates kits – the first to be made available via Addgene and ...gates built from transcriptors are available at Addgene as a BIOFAB kit comprised of well-characterized... promises and associated sequence information. Addgene has partnered with the BioBricks Foundation to ...We have found an amazing and valued partner in Addgene," Endy added. "The phrase we like to use is 'the...programming life, starting with the central dogma. Addgene: And the BIL gates collection? Endy: The Boolean...

  12. Our Most Popular Blog Posts from 2019

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    Blog Post
    ...proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview by Erik Snapp Addgene depositor...2020! Additional resources on the Addgene Blog Read other Addgene news Find the most recent plasmid ...posts Resources at Addgene Browse the repository Find educational resources at Addgene Search for plasmids...discovery. We’ve documented many new tools on the Addgene blog and have created and updated guides on various...plasmids at Addgene ...Angela Abitua Thanks to a team effort from the Addgenies, we’re able to feature some recently deposited... by leaving a comment or emailing us at blog@addgene.org.  Thank you again for a fantastic year and happy...

  13. Expanded Access to Rabies Plasmids

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    Blog Post
    ...Addgene is excited to announce we now offer an export license application for rabies materials! Some ... export-controlled materials in the USA, where Addgene is located.  The export license, if granted, allows... export control license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences...project when purchasing export-controlled materials. Addgene will then apply for the license for distribution...processing costs. Once the application is approved, Addgene then ships the material following our normal process... If you have any questions about this or other Addgene shipping and ordering processes, don’t hesitate...contact our friendly customer support team at help@addgene.org.  Curious about how rabies plasmids are used...

  14. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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    Blog Post
    ... shared mission of both Seeding Labs and Addgene. The Addgene-Seeding Labs plasmid grant is a collaborative...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for.... Additional Resources on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check...introduce the 5 scientists who were awarded the Addgene-Seeding Labs Plasmid grant this year. These scientists...protect fresh water repositories in Mexico. At Addgene we're happy to be able to support diverse groups...Check Out Previous Addgene-Seeding Labs Plasmid Grant Awardees Listen to Our Interview with Previous Awardee... Awardee Kwabena Duedu Resources at Addgene.org Check out our CRISPR resources and CRISPR for plants...

  15. Using Addgene's New Viral Service for Your Research

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    Blog Post
    ...excited that Addgene is now offering virion preparations to the scientific community. Addgene's AAV preparations...posts on the blog and pages on the Addgene website. blog.addgene.org The blog provides tips for working...email us and we’ll get you writing!     www.addgene.org On the Addgene website you’ll find our updated viral... Additional Resources on Addgene.org Overview of Viral Production at Addgene Find Virus Protocols Browse...can now order ready-to-use virus directly from Addgene! As part of our new Viral Service, we’re distributing...preparations undergo rigorous quality control testing at Addgene meaning they come ready made to accelerate your...a wide variety of tools already available from Addgene as ready-to-use viral preps and we’ll be continually...

  16. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    Blog Post
    ...on the Addgene Blog Learn More About the Addgene-Seeding Labs Plasmid Grant Check Out Addgene-Seeding...of the Addgene-Seeding Labs Plasmid Grant awardees' projects progress. Subscribe to the Addgene blog for...Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources, equipment...resource-limited settings. More specifically, the Addgene-Seeding Labs plasmid grant provide plasmids to ...to these researchers. In the third round of the Addgene-Seeding Labs plasmid grant we are excited to introduce...its position in the river back to the user.   At Addgene we're happy to be able to support diverse groups...educational resources for scientists globally, Addgene has partnered with various community organizations...

  17. A Novice's Perspective on Neuroscience - SfN2017

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    Blog Post
    ... to our viral vector pages on the Addgene website and on the Addgene blog to get some ideas for future...Neuroengineering Primer on Optogenetics Resources at Addgene.org Addgene's Viral Service Find Retrograde AAV Browse... Addgene recently attended the Society for Neuroscience 2017 conference in Washington DC (#SfN17). This...you can apply it in neuroscience. AAV tools from Addgene are already having an impact on the field Not to...horn too much, but many people who came to the Addgene booth (there were over 400 who signed up for our...virus (AAV) and lentivirus are now available from Addgene. While super useful, these vectors require a bit...vectors in the repository, you can instead have Addgene make the virus for you. Neuroscientists are particularly...

  18. How to Lead a Great Meeting

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    Blog Post
    ...Read Other Inside Addgene Posts Resources on the Addgene Website Meet the Addgene Management Team Join...Additional Resources on the Addgene Blog Get Management Advice from Addgene Executive Director Joanne ...meetings. Make sure to keep them manageable. At Addgene, teams can add as many points as they like to the...the meeting, take notes on the agenda points. At Addgene, we like to do this live on the screen using a ...review or project plans, before the meeting. At Addgene, we like to send around a Google doc where people...provided some tips above for what works well at Addgene, but meetings are never one-size-fits all. Feel...Join the Addgene Community ...

  19. From our Table to Yours: An Inside Look at Lunch at Addgene

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    Blog Post
    ...sharing Addgene is all about helping scientists share between labs, and people work hard at Addgene to make...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ...as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can only provide... started Hack Days. During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams...a year, she’ll mix up the names of everyone at Addgene, and send random sets of 4 people out to lunch.... to gather around a large table to eat with my Addgene family every day. Do you have any traditions that...

  20. Bringing Renewable Energy to Addgene

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    Blog Post
    ....  Additional resources on the Addgene blog Subscribe to the Addgene blog Read our sustainability in...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...only sustainability effort we’ve implemented at Addgene. The kitchen and office areas provide multiple ... the lab as well. For a more in-depth look at Addgene’s current lab-oriented environmental efforts check...resources by sharing plasmids Find plasmids at Addgene ... washed or reused/repurposed in some fashion. Addgenies swapping out printer cartridges can place the ... about conservation in the lab Resources on Addgene.org Sign up for our quarterly newsletter Help other...

  21. Trends in CRISPR and SynBio Technologies

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    Blog Post
    ...program are here. This was a great opportunity for Addgene to present our own data on plasmid deposits and...and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid repository. Over ...Over 2,000 labs have deposited plasmids to Addgene and we distribute over 130,000 plasmids in 2014. Thus,...combination of open-source distributors such as Addgene, as well as a number of online user forums such...Meeting Eric presented a poster with data comparing Addgene global deposit and distribution trends for Zinc...Addgenie Eric Perkins attended the recent Keystone Meeting "Precision Genome Engineering and Synthetic...You can see these data in the slide share. Addgenies are proud of the role we are playing in advancing...

  22. A Better Way to Get Customer Support: The Help Center

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    Blog Post
    ...comes first. Resources on the Addgene Blog Read other Inside Addgene Posts Learn about New Features ... at Addgene Visit the Help Center Find Plasmids for Your Research Become a Member of the Addgene Community...Addgene’s customer service philosophy Customer service can be a hassle. You finally make it through a... familiar? Well, when it comes to working with Addgene, we certainly hope not! One of our core values ...excited to make these new features available to the Addgene community, and we’d love to know what you think...provide based on your valuable feedback. This is Addgene after all, and making your research easier always...found at help.addgene.org, and the widget is on any page on our regular site, www.addgene.org. We’re excited...

  23. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

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    Blog Post
    ... Joining the Addgene crew!  This pattern was consistent: everyone I spoke with at Addgene, no matter their...hunting and interviews, I accepted a position at Addgene as a marketing and social media co-op. There are...realized I had made the right decision to choose Addgene for my co-op, but I know it didn’t take long. It... It may have been at one of my first “All Addgene” meetings, when Melina, our CSO and co-founder, approached...analytics, something that had never been done at Addgene before. This meant I was responsible for establishing...strategy. I really believe that the culture at Addgene cultivates trust between coworkers and that trust...retrospect, nonsensical. I have found the people at Addgene have become my friends, mentors, and some of my...

  24. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post
    ...things are easy with CRISPR. Addgene: You’ve made many deposits to Addgene over the years. Can you describe...biology, the tools he’s making available through Addgene, and where all of it is likely to lead us in the...the future. Building circuits from CRISPRs Addgene: What can CRISPR do for synthetic biology? Voigt...conceivably be as large as natural regulatory networks. Addgene: What kinds of things could we do as genetic circuits.... It requires more complex synthetic networks. Addgene: What hurdles did you have to overcome and what...regarding the size of the networks you could build. Addgene: Is this what you are working on now? Voigt: We...taking on very large genetic engineering projects. Addgene: How easy is it to do this now? Voigt: One of the...

  25. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...If you’ve ordered a pooled library from Addgene, you may have been a little surprised at how this powerful...deposited many types of pooled libraries here at Addgene, including cDNA, barcoding, screening, and gRNA...gRNA libraries. Most of the pooled libraries at Addgene are lentiviral pooled libraries, though other types...types exist.  When you buy a pooled library from Addgene, you can either order a DNA pooled library, which...single tube, or a pre-made viral prep library. Addgene’s pooled libraries are typically DNA preps, but ...Amplification When you request a DNA pooled library from Addgene, you’ll usually receive an aliquot from the original...similarly-sized aliquot from an amplification done by Addgene.) This small aliquot is not enough to transfect...

  26. New Viral Vectors - Spring 2025

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    Blog Post
    ... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...Addgene’s viral vector services allow scientists to conduct experiments faster, skipping the preparation...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...different serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...

  27. #DeckTheLab with Tinsel and Test Tubes, Fa La La La La La La La La

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    Blog Post
    ...Follow @Addgene, use the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will ...It’s finally December, and here at Addgene HQ in Massachusetts that means it’s time for near-freezing...winner, you’ll receive a winter care package from Addgene.  Here are the details:   Contest 1: Deck the ...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com...however you celebrate! And if you want to see how Addgene celebrates and decorates, follow us on Twitter ...

  28. New Podcast Segment: Hot Plasmids

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    Blog Post
    ...breaking into more audio and video on the Addgene Blog and Addgene website. As we push forward with these...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...present plasmid info with a new segment on the Addgene podcast - The Hot Plasmids Segment. Click on the...Additional Resources on the Addgene Blog Listen to Our Podcast Interview with Eye Researcher Connie Cepko...Science Videos from Tom McFadden Resources at Addgene.org Sign up to Receive Our Newsletter Check Out ...

  29. Streaking for Single Colonies: The Streak Plate Challenge

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    Blog Post
    ...the blog Resources on Addgene.org Find fluorescent protein plasmids at Addgene Find chromoprotein plasmids...a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave W will get vaccinated...found the chromoprotein plasmids available from Addgene, he knew it would be a perfect fit for the streak...biology. “What I'd like to do with the plasmids from Addgene is have the students transform them into different... Youtube:     Additional resources on the Addgene blog Read our other blog posts on fluorescent ...plasmids at Addgene Check out additional written and video protocols ...

  30. New Antibodies in the Lab Animation!

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    Blog Post
    ... resources at Addgene Addgene's Antibody Guide What is an Antibody? animation Addgene's Antibody 101 eBook...sure where to start or what your options are? Addgene's latest animation is here to help! "Antibodies...

  31. We’re Going Viral This Week!

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    Blog Post
    ...and learn more about Addgene’s viral service. Additional resources on the Addgene blog Download our Viral...applications, and some new resources available from Addgene. There’s a lot to talk about! And by the way, there...reagents and data, we’re also publishing research. Addgene’s research team also investigates new strategies... posts Learn more about AAVs Resources on the Addgene website Visit the AAV Data Hub Learn more about...service, and now we’re “going viral” again! #AddgeneGoesViral on Twitter Starting today, our Twitter feed... in the graphic below. Follow the hashtag #AddgeneGoesViral to find information on the basics of viral...

  32. Addgene Launches Partnership with IPI

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    Blog Post
    ...Addgene is excited to announce the launch of our partnership with the Institute for Protein Innovation... of integrin antibodies, will be available on Addgene’s website this fall. IPI antibodies will come ... antibody sequences, in line with the rest of Addgene’s recombinant antibody collection, as well as access...developed to support use of their antibody tools. Addgene also offers a growing collection of educational...

  33. New and Upcoming Viral Vectors - Spring 2019

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    Blog Post
    ...variant of jGCaMP7 in AAV1 at Addgene! See all calcium sensor AAV at Addgene. GABA sensor AAV GABA is the...pAAV-hSyn-mCherry at Addgene. Viral vectors coming soon! These vectors listed below should be released at Addgene in the...viral vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols...the iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical... Find Archon1 and its cre-dependent version at Addgene! Fluorescent protein and control AAV These fluorescent...is reduced.  Additional resources on the Addgene blog Read all of our viral vectors blog posts ...item is available for order please email help@addgene.org with the vector name and serotype you’d like ...

  34. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Blog Post
    ...Doudna, Feng Zhang, and Keith Joung. All Addgene depositors! Addgene was mentioned specifically in Dr. Zhang's...harnessed. Sharing CRISPR, synbio, & more via Addgene Addgene's addition to the poster session was a first...happens): Science is hard. Chris Voigt, another Addgene depositor, discussed some of the many challenges...Simon Singh's "The Code Book" for this month's Addgene Book Club. I read most of the book on my way to...from those who expressed gratitude for the role Addgene plays in plasmid sharing to those who were anxious...increasingly important field.  Interested in Addgene's Keystone Poster?  We'll be sharing the poster...website.  I want to offer a huge thanks to all the Addgenies who worked so hard to make this poster, offered...

  35. Editor's Choice, September 2016

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    Blog Post
    ... that we once again reached new heights on the Addgene blog: we surpassed 60,000 views for the month of... would love to see more posts like this on the Addgene blog. Read the post for the full details and many...,” was contributed by Kurt Swanson, husband of Addgene Director of Biology, Lianna Swanson, and researcher...of therapeutics. It also represents a first for Addgene - while we often participate in 5Ks throughout ...Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate a Scientific...Through Chemistry & Small Molecule Fluorophores Addgene's Cancer Collection Pages Connect the Research Community...Francisco and would like to give my thanks to all the Addgene supporters who spoke with me and deposited plasmids...

  36. New Viral Vectors - March 2024

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    Blog Post
    ...latest in Addgene's viral vectors services and resources!   More resources on the Addgene blog Addgene's...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...the full viral services collection or check out Addgene's Data Hub to view viral vector reports from other...

  37. Visualizing Your Plasmid Request Data

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    Blog Post
    ... use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The Addgene Requests Dashboard...I was excited to see that Addgene has recently added a new feature for depositing labs to its website...default. Depositors can download their CSV file from Addgene by following these instructions, and then uploading... 2: Insert showing the upload function for the Addgene Requests Dashboard After the upload, the name...displays the data in a standardized way. Since Addgene encourages the public sharing of aggregated data...

  38. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

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    Blog Post
    ...Instrumental Access participants. Like Seeding Labs, Addgene also feels it’s incredibly important for scientific...distribution and disseminate knowledge around the globe. Addgene’s progress in international distribution As you ...are making great progress toward spreading the Addgene community into developing regions like South America...the countries that have requested plasmids from Addgene since our inception in 2004. In the near future...SeedingLabs or on Facebook Additional Resources on the Addgene Blog Learn About Our Recent Visit to China Read... Institutions that Deposit their Plasmids with Addgene ...People at Scientific Conferences Resources on Addgene.org Read about Global Plasmid Distribution Check...

  39. 500,000 Plasmids Shipped and Counting!

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    Blog Post
    ...shipping look like at Addgene? Here's an inside peak into the daily workings in Addgene's lab. We ship individual...March 26, 2015. How does Addgene make it happen? Our awesome team of Addgenies! Our team recently grew ...Yesterday we hit a huge milestone here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids...with shipping labels and paperwork. Our stacks of Addgene boxes are now ready to be sent around the world... comments from our blog readers. You all drive Addgene to succeed and to improve, so thank you. 500,000x... to necessitate an office move. We've got ~50 Addgenies working to ship plasmids, analyze QC sequence,...500,000x Thank You! Have a question? Email: help@addgene.org Call: +1 (617) 225-9000         ...

  40. Enabling high school research at the Journal of Emerging Investigators

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    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... In this episode of the Addgene Podcast, we introduce you to the Journal of Emerging Investigators, an...recently submitted to the journal with funding from Addgene. Listen to the interview with the Journal of Emerging...Additional Resources from the Addgene Blog Find education resources Use our Plasmids 101 eBook to teach ...genome enegineering technology Resources at Addgene.org Find basic molecular biology protocols Check...

  41. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post
    ...plasmids from this paper at Addgene. Additional Resources on the Addgene Blog Browse Our CRISPR Topic...add multiple gRNAs on one plasmid. Sound good? Addgene has many tools to help you multiplex - we’ll use...Multiplexing gRNAs: the basics One common question Addgene Senior Scientists receive is: can I express more...Two Golden Gate options that are available from Addgene follow these same assembly principles, but they...cassette.    Find gRNA multiplexing vectors at Addgene! Multiplexing in plants Qi-Jun Chen Lab Golden ... PMC4270925. Find plasmids from this paper at Addgene. 2. Kabadi, Ami M., et al. “Multiplex CRISPR/Cas9... PMC4231726. Find plasmids from this paper at Addgene. 3. Sakuma, Tetsushi, et al. “Multiplex genome...

  42. Addgene Stands with the Scientific Community

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    Blog Post
    ...utilizes grants to support our own initiatives, Addgene is deeply concerned about these developments. These... We Are Here to Help Despite these challenges, Addgene remains steadfast in our mission to accelerate ...vital during this period of constrained funding. Addgene is uniquely positioned to help research institutions...Universities and research institutions can turn to Addgene as a trusted partner for archiving their biological...scientific progress depends on our collective response. Addgene stands ready to collaborate with partners across...work and what support would be most valuable from Addgene during this time. Your input will directly shape...scientific community. Let us know by emailing help@addgene.org. We look forward to hearing from you!...

  43. MassBioEd Career Exploration Day at Addgene

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    Blog Post
    ...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from...that Addgenies represent. They kicked off their day with breakfast and an introduction to Addgene with...to start networking! Learning about careers at Addgene Afterwards, the students split into two groups ...groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology...with the “comfortable surroundings” of the new Addgene office space.  Thanks Abigail, we think it’s pretty...excited to see a real working lab full of busy Addgenies preparing plasmid samples for shipping. This was...with a biology major.” After hearing about many Addgenies’ career paths, Michelle Healy said “You are always...

  44. Genome-wide Screening Using CRISPR

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    Blog Post
    ...that have been made publicly available through Addgene.  What makes CRISPR so special? A major advantage...on the various CRISPR technologies, check out Addgene's CRISPR Guide. Figure 1: Like this jar of...CRISPR libraries are publicly available through Addgene, with more being added all the time. Keep in mind...species are your cells derived from? Currently, Addgene carries CRISPR libraries that target mouse, human...What genetic modification are you trying to make? Addgene carries CRISPR libraries for gene knockout, activation...gene in the genome, or a specific class of genes? Addgene currently carries several genome-wide CRISPR libraries...obtained a ready-to-use lentiviral preparation from Addgene, you can skip the steps above! Cells are then transduced...

  45. Imaging Tools and Following a Passion for Basic Biology: Interview with Joachim Goεdhart

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    Blog Post
    ...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.      Additional... Today on the Addgene Podcast, we have another interview conducted by European Outreach Scientist Benoit...Additional Resources from the Addgene Blog Visit our Fluorescent Proteins featured topic page Read Joachim's... addgene podcast...Subcellular Structures and Organelles Resources at Addgene.org Find Fluorescent Protein Plasmids Find Plasmids...

  46. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    ...intern who shared their experience working at Addgene. An Addgene Summer Internship Matteo Tardelli, who ...contributing to the Addgene blog? We'd love to hear from you! Email us anytime at news@addgene.org. ... very thankful time of year for those of us at Addgene! And we can’t think of any better way to start ...to the blog. Thank you! More resources on the Addgene blog 10 Great Guest Posts We're Thankful For Lucky...

  47. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...When I got the job of Outreach Scientist at Addgene, my first thought was “I get to talk to scientists... spend a lot of time telling scientists about Addgene’s repository, and explaining how to deposit and ... 1,600+ labs around the world!) that have kept Addgene at the forefront of life sciences research for ...from scientists who have deposited plasmids at Addgene.  Learn how discoveries were made and get practical...scientific sharing and the open-access movement. At Addgene, we believe that science gets better when everyone... to tell? Let us know by emailing us at blog@addgene.org or tell us on Twitter and Facebook. We’ll track...

  48. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    ...requested from Addgene. Figure 2: Total COVID-19 plasmids requested from Addgene from March 23, ...resources on the Addgene blog Read more about COVID-19 databases and resources Learn about Addgene's work to ...all scientists Resources on Addgene.org Find COVID-19 plasmids at Addgene Browse the entire plasmid collection...2020). The authors requested these plasmids from Addgene. This publication then resulted in the development...3X-Flag-SARS-CoV-2 S, which is now easily available through Addgene and has already been requested dozens of times ...develop available to the scientific community. Addgene was awarded a Fast Grant to ensure that the scientific..., 2020 to July 27, 2020. Browse Addgene's COVID-19 plasmids and resources! Case study 3: Mouse strains...

  49. Early Career Researcher Toolbox: Social Media for Scientists

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    Blog Post
    ...Before I started writing for the Addgene blog, sharing Chemistry Cat memes was how I used social media...social media as a scientist. Now I write for the @Addgene blog & I'm my lab's resident #scicomm expert. What...using social media as a scientist started with the Addgene blog. I wrote my first blog post out of boredom...person in the lab. When I saw a request for an Addgene guest blogger post about colony PCR, I signed up...and accepted a position to work remotely as an Addgene Science Communication Intern. Developing science... using Twitter, I found this paper. I wrote an Addgene blog post about it and shared the paper with my... this panel, but my consistent writing for the Addgene blog (~1-2 blog posts per month) and use of Twitter...

  50. New Viral Vectors - Fall 2024

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    Blog Post
    ...preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged in the... Brian O'Neill.  More resources on the Addgene blog Addgene's Data Hub What is a Virus (and a Viral Vector...Table 1: New ready-to-use viral vectors in the Addgene repository  Name Serotype Category Depositor...the full viral services collection or check out Addgene's Data Hub to see viral vector reports from other... the link below to keep up with the latest in Addgene's viral vector services and resources!   This blog...
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