Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

We narrowed to 127 results for: 1-Oct;

Showing: 101 - 120 of 127 results

  1. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type
    Blog Post
    ... such a popular model organism? Zebrafish (Figure 1) were initially recognized as a useful vertebrate ... post was contributed by Katherine Rogers, a postdoctoral researcher at the Friedrich Miescher Lab of ...Max Planck Society. Katherine W. Rogers is a postdoctoral researcher in Patrick Müller’s group at the ...

  2. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...blood test for HMGB1, the high mobility group box 1 protein. Normally localized to the nucleus, if the... for years, or they are exposed even one time. Doctors and researchers don’t entirely understand why some...exposed to asbestos Tests like these will allow doctors not only to detect mesothelioma much earlier, thus...

  3. March for Science

    Type
    Blog Post
    ...education, government, and civilization in general (1). Science and politics? Science is an apolitical process...consequences of a march. Three of these risks include: 1) Lost trust Perhaps the greatest risk is that the ...active research scientists (scientists who hold a doctorate degree, are currently working full-time, and have...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...SpCas9 base editors with editing windows as small as 1-2 nucleotides! To reduce off-target effects associated...Lower on-target activity than BE3; editing window ~1-2 nt  87438  pET42b-HF-BE3  HF-Cas9 (NGG)  Very ... through the expression of transcription factors OCT3/4, SOX2, KLF4, and c-MYC is the established model...Addgene and include a variety of cMYC, KLF4, SOX2 and OCT4 DNA binding domain and transcription activation ...

  6. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...breaks (DSBs) or donor DNA templates.  Figure 1: An overview of prime editing. Created with BioRender.com...demonstrated ~20-50% efficiency in HEK293T cells with 1-10% indels. Since then, further innovations have continued...Engineering. https://doi.org/10.1038/s41551-024-01227-1 Yan, J., Oyler-Castrillo, P., Ravisankar, P., Ward...post was originally written by Jennifer Tsang in October 2019 and updated by Emily P. Bentley in December...

  7. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... After all I was only going to be interviewed for 1 hour so having a broad enough insight was going to.... I had just started month 8 of my 3 year Post-Doctoral contract at the University of Cambridge and knew...

  8. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...process is explained more fully in the animated Fig. 1 below. Normalization is a fundamental and often understated...Bioinformatics from Boston University, and did postdoctoral work at Harvard Medical School and the University...

  9. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...the guides are relatively unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences...candidates for further development. They inserted an octet of MS2 hairpins into the gRNA tetraloop and went...

  10. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...regarding what makes this group of researchers special: 1. Stem cell scientists care deeply about ethics Ethics...differentiated cells simply by expressing four proteins Oct4, Sox2, Klf4, and cMyc (the so called OSKM factors...

  11. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important...Management Software Resources on Addgene.org Find Protoctols for Your Research Catch Up On Your Molecular ...

  12. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...advantage this cycle, consider the advice below:  1. Eligibility check Scrutinize every opportunity prior...Pierre! Roodolph (Roo) P. St Pierre is currently a doctoral candidate in the Chemical Biology Program at Harvard...

  13. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...many of which are available in rAAVs!   Figure 1: Diagram of AAV components      Cargo capacity...Guerin and Susanna Stroik. Didem Goz Ayturk is a Postdoctoral Fellow in Connie Cepko’s Lab investigating neuronal...

  14. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...Bachelor's degree Generally a short-term position (1-2 years) Often a gap-year position taken to transition...teaches graduate and/or undergraduate classes Postdoctoral researcher (postdoc) Getting more scientific...scientific and professional training after earning a doctorate Considered a "stepping stone" position to become...

  15. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    ...basis of many molecular biology tools! Figure 1: Common functions of natural plasmids. Created with...January 2017 and was updated by Emily P. Bentley in October 2024. References and Resources References Demanèche...

  18. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...collaborations, keep these jobs in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation...keeping track of speakers. During your graduate or postdoctoral work there are loads of opportunities to create...

  20. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...avoid breathing in the vapors. Workflow Timeline Day 1: Antigen Coating Day 2: Blocking Day 3: Primary antibody... Spectrophotometer compatible with 96-well plates 1–10 µL single channel pipette 2–20 µL single channel... µL, VWR 76322-134 Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, Corning 430518 PBS, 1X pH 7.4...Warm reagents to room temperature. Procedure Section 1: Prepare the Antigen Standard and coat the plate Dilute...stock into 900 µL PBS in a microfuge tube and vortex. 1 ng/µL : Add 450 µL of 2 ng/µL stock into 450 uL PBS...microfuge tube and vortex. 0.5 ng/µL : Add 450 µL of 1 ng/µL stock into 450 µL PBS in a microfuge tube and... a microplate shaker set at 400 rpm and shake for 1 min . Repeat steps 8–10 twice for a total of three...
Showing: 101 - 120 of 127 results