We narrowed to 161 results for: ALC

1. Hot Plasmids and Viral Preps - March 2021

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   ...INTRSECT project. Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f...

2. Hot Plasmids February 2024

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   ...doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains...

3. Viral Vectors 101: Chemogenetics

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   ...doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri M, Ursu D, van der Veen B, Liss B, Kätzel D,...

4. Viral Vectors 101: Virus Safety

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   ..., which are resistant to ethanol and isopropyl alcohol, 0.5% peracetic acid, 10% bleach, or 1% iodine ...

5. Hot Plasmids - October 2020

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   ...AAV5. Find the AAV prep here The red-shifted calcium sensor jRGECO1a is now available as AAV-PHPeb. ...

6. Fluorescent Proteins 101: Introduction to FRET

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   ..., 1997), was designed to measure intracellular calcium and published in 1997. Since that time, numerous...

7. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ... Lysine (D123K), lowers biosensor affinity for calcium.”  Primary Vector Type The vector type should...

8. Pooled Library Amplifications

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   ...transformation efficiency, or more specifically calculated based on the size of and weight of your library...

9. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   ...used so far) or for cancer gene therapy such as nonalcoholic steatohepatitis cancer. In deficiencies of plasma...

10. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    ...transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines Transformed...

11. Light Sheet Fluorescence Microscopy

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    ...Spalteholtz (10).  He used a solution of benzyl alcohol and methyl salicylate which was later modified ...

12. DNA Purification Without a Kit

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    ...proteins and RNase. Finally, DNA is precipitated with alcohol and resuspended in TE buffer. After this, the DNA...

13. Tips for arabidopsis transformation

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    ...enough to dunk your plant’s flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options...

14. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...18845710. Kim, Dal Hyung, et al. "Pan-neuronal calcium imaging with cellular resolution in freely swimming...

15. Analyzing CRISPR Editing Results with ICE from Synthego

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    ...guide RNA sequence you used. The ICE tool will calculate overall editing efficiency and determine the profiles...

16. AAV Titers: Where Do They Come From And What Do They Mean?

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    ...measured by qPCR.   Physical titer is typically calculated by two popular PCR-based methods: Quantitative...

17. Data Freedom: The Expansion of Data Sharing in Research Publications

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    ...representative or the statistical variation is calculated and placed in the legend. Widely reported difficulties...

18. Antibodies 101: Normalization and Loading Controls for Western Blots

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    ... later steps in this chain (blue) to infer or calculate things about earlier ones. In practice, multiple...

19. Hot Plasmids: Summer 2024

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    ... S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered virus-like...

20. Filming Science Videos in the Age of Social Distancing

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    ...good once. Even when things return to relative normalcy, I’ll be incorporating that mobile monitor stand...