Flow Cytometry Readouts: Yes, No, and Everything in Between
Type
Blog Post
...low expression, and everything in between (Figure 2). These smears tend to occur when you are looking ...based on your experiment. You can see from Figure 2 that determining where to place your gate based on...the dot plot would be complicated.
Figure 2: (A) a smeary dot plot showing a range of antibody...plot in Figure 4 compared to the dot plot in Figure 2. Quantification using MFI makes it easier to identify...positive samples are clearly distinguishable as two separate populations. Created with biorender.com.
Pro...with the baseline suggesting this sample has a comparable expression to the baseline/negative control.
...expression, using a histogram plot and MFI values to characterize your samples may allow for better interpretation...