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We narrowed to 644 results for: ATO

Showing: 101 - 120 of 644 results

  1. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...crosslinking agent, a signaling ion, and an allosteric modulator. Because of its importance, scientists have developed... a calcium sensor Genetically encoded calcium indicators (GECIs) are fluorescent proteins linked to calcium...in living cells and organisms. Unlike chemical indicators, these DNA constructs can be delivered to intact...:509-18. Inoue M. Genetically encoded calcium indicators to probe complex brain circuit dynamics in vivo...Kanemaru K, Iino M. Genetically encoded fluorescent indicators for organellar calcium imaging. Biophysical journal...neuronal activity with genetically encoded calcium indicators. Cold Spring Harbor Protocols. 2012 Jun 1;2012...signaling using genetically targetable fluorescent indicators. Nature protocols. 2006 Aug;1(3):1057-65. Resources...

  2. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...development programs for bioscience educators. Using these hands-on tools, educators introduce their students to...a backbone structure of a protein, based on its atomic coordinates. These wire models were useful in that...necessary to download a pdb file of the structure’s atomic coordinates, and “open” these structures with molecular...dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models?   We created...something tangible. Under the guidance of a trained educator, these instances of focused attention become magical... their labs. Soon after we began working with educators in our Center for BioMolecular Modeling, we spun...

  3. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...following: the elevator pitch, talks, the poster session, networking, and social media. The elevator pitch Before... Before the conference Practice your “elevator pitch,” the 30 - 60 second blurb you will use to introduce...work you are familiar with. You can tweak your elevator pitch slightly if you know a bit more about the...or talk. After the conference After using your elevator pitch several times at the conference, you might...might notice some trends. Were there parts of your elevator pitch where the other person seemed confused, ...questions. If you or your lab identifies a potential collaborator from the meeting, reach out to them, pointing...

  4. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ...The first is the study of non-coding regulatory elements. Regulatory DNA function is typically inferred ...bloggers! Daniel Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and...interested in exploring the function of non-coding regulatory elements in their native chromatin context. An...PhD student at Harvard Medical School in the laboratories of Stuart Orkin and Daniel Bauer. He received...

  5. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...employing optogenetic regulators to initiate the event being monitored. Optogenetic regulators respond to specific...initiation wavelengths, excitation of the optogenetic regulator leads to spurious activation of the FRET sensor...interested in using photoactivatable, optogenetic accuators to depolarize or hyperpolarize membranes. Traditional...wavelengths needed would also activate the optogenetic accuators. The researchers took a FRET reporter and adapted...biosensors that can even be paired with optogenetic regulators. Many of these tools are available from Addgene...2017) Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging...

  6. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    ...lipoproteins, shuttles lipoproteins through the circulatory systems, but can also lead to the formation of...as both a powerful tool for generating complex regulatory systems and further the study of natural σ54-... PMID: 31451697. Genetically encoded voltage indicators for use in worms Article contributed by Brook...Brook Pyhtila Genetically encoded voltage indicators (GEVIs) change fluorescence in real time when neurons... excitation light intensities have made these indicators difficult to work with.  The Gottschalk lab has...microbial rhodopsin GEVIs in excitable cells of the nematode Caenorhabditis elegans. They have found several...

  7. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...upon a moratorium until another meeting, three branches – a private funder, government regulators, and ...there was a generally agreed-upon international moratorium on utilizing CRISPR/Cas9 in humans. This topic...mtDNA transfer options being investigated; A UK regulatory body tasked two separate non-governmental groups...technology to research centers engaged in long-term, mandatory follow-ups and observational studies of the technique...stop punting decisions on scientific, ethical, regulatory, and general public consensus to the next meeting...

  8. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...site-specific recombinases, regulatory elements, developmental regulators, and lots of viral components...test many different combinations of promoters, terminators, solubility and purification tags, connectors... examine the effects of different promoters, terminators, enhancers, and cross-cassette interactions in...Standard and New E. Coli Part Library Enable Rapid Combinatorial Design for Synthetic and Traditional Biology... Signaling in CAR T Cells. Cold Spring Harbor Laboratory, 17 Feb. 2024. Crossref, https://doi.org/10.1101...Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref, https://doi.org/10.1016...

  9. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...environmental modulation of hepatocellular cancer cell growth." Hepatology 54.4 (2011): 1237-1248. PubMed... was contributed by Bárbara Pinho, a science communicator at the Portuguese science museum "Fábrica Centro...myofibroblasts, neuroendocrine, adipose, immune, inflammatory, angiogenic vascular, endothelial, and stromal...seems to attack us by releasing mitogenic growth mediators and improving cancer cells proliferation.  How... Pinho! Bárbara Pinho is currently a science communicator at the Portuguese science museum "Fábrica Centro...

  10. Hot Plasmids - October 2020

    Type
    Blog Post
    ...you'll find in this post: Tools for labeling excitatory inhibitory synaptic proteins Fluorescent protein...corner New from the viral service Labeling excitatory and inhibitory synaptic proteins with FingRs ...developed AAV vectors to selectively visualize excitatory and inhibitory synapses in the brain.  ...scaffolding proteins PSD95 and gephyrin to label excitatory and inhibitory synapses, respectively. Once expressed... fluorescent proteins targeting inhibitory or excitatory synapses or that are Cre-inducible, researchers... cycle in vivo, particularly if the cells are migratory or in deep tissues. To resolve this issue, Shangqin...

  11. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ... the notation works? The + operator denotes intersection and the – operator denotes set difference. That...inclusive markers of regulatory regions in the genome, including enhancers, promoters, insulators, and more. I...enhancers, lncRNAs, repetitive elements, repressors, insulators, and more. We know that this matter exists, but...criteria: no TTTTT allowed (this is a polymerase terminator), and no off-target effects for the identified...

  12. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...discovered the modular format of some transcriptional activators: that the DNA binding domain (DBD) and transcriptional...of whether it interacts with a transcriptional activator (1a) or a repressor (1b). Scientists took advantage... original Y2H systems utilized the yeast Gal4 activator, bacterial LexA DBD and the lacZ reporter gene...transcription-activating function of a eukaryotic regulatory protein. Science. 1986; 231:699-704. PubMed PMID... and Ptashne, M. A eukaryotic transcriptional activator bearing the DNA specificity of a prokaryotic repressor...PubMed PMID: 2547163. 5. Ozawa, T., Kaihara, A., Sato, M., Tachihara, K., Umezawa, Y.l. Split luciferase...

  13. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    ...C-terminal tag; TER, terminator. Created with BioRender.com. The Sainsbury Laboratory Golden Gate Cloning...construct a Level 1 vector with a promoter and terminator flanking the CDS, and a Level 2 multigene vector... of interest a promoter, a coding sequence, a terminator, or other plasmid part? Do you want to fuse your...these would be interchangeable between different laboratories. Therefore, choosing acceptor vectors and parts... from Addgene will most likely arrive in your laboratory as plates of frozen glycerol stocks. It is very...

  14. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ... concrete happen. You may have more willing collaborators than you realize. Being at a university provides...meetings and take minutes. Get to know your collaborators from other institutions personally on conference...relationship with the perspective of my potential collaborators in mind. Being at a university allows you to...with people at your organization. You can meet collaborators  for lunch, make a point of talking to them ...as messages and keep you up-to-date on your collaborators’ professional developments without directly ...to remind myself to check in regularly with collaborators for example on a holiday or for professional...

  15. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...has a great team of moderators who facilitated scheduling, filtered out inflammatory questions, and even...and the moderators were on top of any comments that were at all trollish. Kudos to the moderators for keeping...lists of forums on particular topics. Volunteer moderators set the rules for these forums and try to keep...days). Redditors ask serious questions What the moderators did not delete or suppress were the large number...

  16. Plan Your Experiment

    Type
    Collection
    ...fusion with transcriptional activator (such as dCas9-VP64) Many different activator systems exist, including...requires dCas9 to be fused to transcriptional activators that need to be within a given range of the transcription...

  17. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...calcium indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs...performance, and applications of near-infrared calcium indicators in this recent review (Shcherbakova et al., 2021...encoded near-infrared fluorescent calcium ion indicator. Nat Methods 16, 171-174 (2019). Matlashov, M..... A near-infrared genetically encoded calcium indicator for in vivo imaging. Nat Biotechnol 39, 368-377...Near-infrared and far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362,...

  18. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...crop species including potato, corn, tomato, wheat, mushroom, and rice (Khatodia et al., 2016). Despite...https://doi.org/10.1128/mmbr.67.1.16-37.2003 Khatodia S, Bhatotia K, Passricha N, Khurana SMP, Tuteja N (2016... al., 2013). Nuclease dead Cas9 (dCas9) based activators (such as dCas9-VP64) or repressors (dCas9-KRAB...

  19. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...expression. Fusing dCas9 to a transcriptional activator like VP64 or VPR activates transcription, whereas... is their persistence and inheritance. CRISPR activators and repressors are thought to be reversible once...cases, epigenetic modifiers may work better than activators/repressors - dCas9-p300 increased transcriptional...enables high-throughput screening for functional regulatory elements in the human genome. Nature Biotechnology...engineering CRISPR toolkit for probing in vivo cis-regulatory interactions in the chicken embryo. BioRxiv: ...Additional Resources on the Addgene Blog Cas9 Activators: A Practical Guide CRISPR Between the Genes: ...

  20. Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies

    Type
    Blog Post
    ...have begun to use more precise genetic tools: activators, inhibitors, sensors, recombinases, and more....which was recently published in Frontiers in Neuroanatomy.  Here are the three main takeaways from the...achieved in nontransgenic animals by using cis regulatory elements such as promoters and enhancers, multicistronic...multicistronic vectors, and post-translational regulatory elements. Examples of these approaches are given...specificity of the targeted cell population.  Combinatorial strategies can also be used for retrograde tracing...
Showing: 101 - 120 of 644 results