...cell Plasmids 101: Green Fluorescent Protein (GFP) Plasmids 101: Luciferase Monitor the level of...for plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...found in bacteria, but also exist naturally in archaea and eukaryotes such as yeast and plants. In nature... Produce synthetic viruses that can be used in research or for therapeutics Addgene has compiled various...

...Addgene’s western blot for the myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications...     HcKCRs represent a long-sought tool for researchers to control K+ gradients and can be used as a ...of the most commonly used protein tags - a quick search for ‘Myc tag’ in the Addgene catalog pulled up ...

...transcription activation systems, such as LexA/p65 or mCD8/GFP, to allow sparse labeling in a variety of fly cell...no completely genetic tools that would allow researchers to label just a fraction of a single genetically-defined...

..., hygromycin) and fluorescent protein reporters (GFP, BFP, RFP, no FP). The vectors each contain two SfiI... Molecule of the Year. Additionally, in 2014 researchers at MD Anderson Cancer Center presented results...

...or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9,...restricting the availability of Cre, lox sites lets researchers control the spatial and temporal activation of...

...Viviana Gradinaru 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic...pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal indirect pathway...pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) Expression of CoChR-EGFP in striatal direct pathway...

...option rtTA On Kowarz 16623 pBI-GFP Expression of your gene of interest & GFP from a bidirectional tet-responsive...Either Lung 11662 pPRIME-TET-GFP-FF3 Lentiviral, miRNA expression (PRIME) system for application in knockdown...cells; Expresses firefly luciferase hairpin and GFP under pTREtight promoter None Either Elledge 35625...Safe Harbor Locus. Tet-On 3G On Doyon 58245 pGLTR-X-GFP Single vector lentiviral Gateway RNAi system for ...generation; contains expression cassette for TetR-P2A-GFP; see article for additional constructs TetR On Geley...inducible promoter at its 3' end, which controls GFP expression tTA Off Verma 26803 pEnt L1L3 EF1a-tTA...16542 pBI-MCS-EGFP Expression of your gene of interest (MCS with a β-globin poly A) & EGFP from a bidirectional...

...collection is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465) are now available...our entire AAV inventory. Our new AAVs include: EGFP-expressing AAV for serotype testing Calcium sensors... testing AAV, which are small (20 ul) samples of EGFP-expressing AAV packaged in various serotypes. This...recently released these controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad...broad, neuronal expression. AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs ubiquitous expression...interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5 and 112677-AAVrg): The EF1a promoter...mCherry in the absence of Cre, and expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium ...

...destination vector contains GFP, enabling you to select cells with high GFP expression. These cells have...Cpf1.   Figure 1: Multiplexing allows researchers to express multiple gRNA from a single construct...from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014): e147. PubMed PMID: 25122746. PubMed... Our Guide Pages Find CRISPR Plasmids for Your Research Browse Our CRISPR Multiplexing Resources ...

...many different restriction enzymes. pPro24-gfp contains GFP but all others are empty. pdCas9-bacteria ...fluorophore-tagged MS2 (such as that found in pZS*12-MS2-GFP ) to track RNA localization of your gene of interest...lactose/IPTG inducible) Flag, His, Strep, mOCR, MBP, GFP, with and without SUMO or TEV cleavage sites to remove...Gram-negative bacteria. pAK Series Various Plasmids pTac GFP, Luciferase (LuxCDABE) Attila Karsi Broad host range...be found here . pAra Series Various Plasmids pBAD GFP Bryan Berger Reporter system for testing hetero- ...AraC-fusion homodimers results in the activation of GFP expression from the araBAD promoter whereas heterodimer...

...the viral protease, Nsp5. Our set also includes a GFP construct as a control. For easier portability, all...Eckhardt and Melanie Brewer from the QBI Coronavirus Research Group at UCSF. It’s been only a few months since... more than 20 labs to form the QBI Coronavirus Research Group (QCRG) to do just this.  In under a month...with requests to share our plasmids with other researchers. We have been excited to see so much interest...problem more quickly. Figure 2: Getting our research in line, with a row of FedEx envelopes waiting...shared spreadsheet and it takes time away from our research. For all these reasons, the QCRG is delighted ...requesting plasmids for coronavirus or COVID-19 research, please email Addgene at [email protected] with...

...fluorescent protein topics page Get the basics on GFP Find microbiology blog posts Resources on Addgene.org...development by openly sharing their resources with the research community. To honor their legacy, we launched ...choice to share their fluorescent protein based research. This year, we put out another call for applications...genetic variants, what does that mean? Previous research shows that approximately 75% of all protein-coding...variant library is monitored by fusing the variants to eGFP followed by an internal ribosomal entry site and...a variant has wild-type levels of abundance, the eGFP will be stable and fluoresce. But if the variant...unstable, it will be degraded, leading to reduced eGFP fluorescence. Cells expressing the library are then...

...library comes either with or without mCherry and GFP fluorescent controls. Alternatively, you can order...including over 3,500 human TF isoforms, to help researchers understand this regulatory network. The transcription... test prophylactics on and a program that any researcher could use to generate the relevant disease model...

...Plasmid 68295 GFP E17TAG Plasmid 68296 GFP S2TAG Plasmid 68297 GFP Q157TAG Plasmid 68298 GFP S2TAG/E17TAG...E17TAG Plasmid 68299 GFP E17TAG/Q157TAG Plasmid 53225 MBP-MEK1 S218TAG/S222TAG ( aka. PCRT7 tetR pLtetO MBP-MEK1...68305 Beta lactamase S68TAG Plasmid 69118 E17TAG GFP zeo resistance Strain 34929 BL21ΔserB Strain 52055...of posttranslational modifications in cells and research into its many roles in protein function and signaling...Söll labs at Yale University changed the way researchers can explore important questions surrounding serine...for testing phosphoproteomics methods, proteomics search algorithms, proteomics phosphosite localization...

...via phosphorylation, promoting transcription of a GFP reporter. Subsequent exposure to red light rapidly...around 100-fold. These improvements will allow researchers to better fine-tune the level of output gene ...

...be another method to assess transfection success. GFP is often used as a reporter and we will be covering...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian...

...cells in vivo, the non-immunogenic violet-excited GFP variant Vex. To understand the impact of this gene...neurotransmitter release. How is this light-mediated? The researchers split the proteolytically active light chain ...of cellular pathways. Despite several years of research, the mechanisms of miRNA regulation have yet to...

...selection cassette. They could also switch from FLAG to GFP or a different epitope. Addgene: Do you have any ... the ENCODE Consortium in Cold Spring Harbor in March. A number of groups were excited and asked for the...

...line, Hep3B. From left to right: Mitotracker Red, GFP for the biosensor, Hoechst, and overlay).   ...German Cancer Research Center (DKFZ) who has developed new redox sensitive GFP2 (roGFP2)-based biosensors...fluorescent proteins in their research. These awards help early career researchers attend a conference of their...opened up applications to research technicians and undergraduate researchers, in addition to postdocs ...postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where..., Pedre Pérez’s biosensor fuses MPST to roGFP2. Because roGFP2 contains two cysteines near the chromophore.... Below, read about the five winners and their research.   Abhi Aggarwal: Glutamate sensing reporters...

...is a higher risk cargo for a researcher to come into contact with than GFP. Similarly, if the viral vector...than cell cultures do, and tired researchers are accident-prone researchers. Note that safety policies will...have become a regular part of basic biological research as well as clinical therapy. These biological ...in non-target cells, including yourself as the researcher.   Figure 1: Separation of retroviral ...need to be done in a biosafety cabinet. Many researchers choose to wear eye protection when they are working...likely to want to cut a few corners. For many researchers, viruses might be the most dangerous things they...