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We narrowed to 7 results for: Arch-GFP

Showing: 1 - 7 of 7 results
  1. Adenovirus Guide

    Type
    Guide
    ...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping The production of viral vectors (or...adenoviral vector production that do not contain GFP. Recombinant adenoviral vector (rAdV) Modified form...Wild-type adenoviruses have been modified by researchers to create recombinant adenoviral (rAdV) vectors...gene expression patterns that were observed, researchers have tended to favor using first- and third-generation...is still the most commonly used generation for research purposes. The system consists of two plasmids ...
  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell ...treatments into cells, and has been widely used in the research community for decades. Wildtype gamma-retroviruses...vectors have many aspects that make them useful research tools in a variety of cell types and models, such...integration, gamma-retroviral vectors are useful research and clinical tools thanks to their long-term expression...practices with regard to gamma-retroviral-related research. See Addgene’s Biosafety Resource Guide for more...Retroviral Vectors and Their Uses in Scientific Research Gamma-retroviral Packaging Systems The Retroviral...
  3. Lentiviral Vector Guide

    Type
    Guide
    ...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...treatments into cells, and has been widely used in the research community for decades. Wild-type lentiviruses ...vectors have many aspects that make them useful research tools in a variety of cell types and models. Lentiviral... of a transgene, lentiviral vectors are useful research and clinical tools. Stable Cell Lines Lentiviral...best practices with regard to lentiviral-related research. See Addgene’s Biosafety Resource Guide for more...accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration...
  4. Optogenetics Guide

    Type
    Guide
    ...peak action spectra Example: Jaws Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense is ...589 Archaerhodopsins Arch Light-activated outward proton pump from Halorubrum sodomense 566 eArch 3.0 ...TP009. Improved light sensitivity over Arch. 566 eArchT 3.0 ArchT with addition of trafficking signal from...3.0 Arch with addition of trafficking signal from Kir2.1 566 ArchT Light-activated outward proton pump from...used to inhibit neurons in optogenetic experiments. Arch is a light-activated outward proton pump that hyperpolarizes...) the cell when triggered by green-yellow light. Arch variants have been developed with the following ...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...
  5. Modular Cloning Guide

    Type
    Guide
    ...a combinatorial library of constructs expressing GFP at 18 levels for rapid characterization in new organisms...MoClo Plasmid Kits Available from Addgene Browse or search the table below to find MoClo kits in our collection...Johannes Stuttmann 95 additional plasmids for hierarchical assembly of plant multigene constructs. MoClo...characterized parts that can be used for bottom-up hierarchical assembly of single and multi-gene constructs...
  6. Molecular Biology Reference

    Type
    Guide
    ...plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout of the activity of the genetic...codon-amino acid pairs. Over the past two decades, researchers have also expanded the genetic code by redirecting...chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant ...phoA supE44 thi-1 gyrA96 relA1 tonA EPI300 LGC Biosearch Technologies; MilliporeSigma F- mcrA Δ(mrr-hsdRMS-mcrBC...information to aid in the reproducibility of scientific research. Long-read Sequencing Sanger sequencing and Illumina...
  7. CRISPR Guide

    Type
    Guide
    ...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...Overview of Cas9n. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert... 5: Overview of base editing. More recently, researchers have developed base editors that can catalyze...
Showing: 1 - 7 of 7 results