...typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells, or selection...transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich positive...Started CRISPR is a powerful system that enables researchers to manipulate the genome like never before. This...framework to get you started using CRISPR in your research. Although we will use the example of CRISPR/Cas9...

...another marker, such as GFP. A researcher can use FACS to sort cells expressing GFP and later expand these...institution's best practices with regard to lentiviral research. The NIH has additional information on lentiviral...

...cell when triggered by green-yellow light. Arch Variants. Arch variants have been developed with the following...Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense is ...589 Archaerhodopsins Arch Light-activated outward proton pump from Halorubrum sodomense 566 eArch 3.0 ...TP009. Improved light sensitivity over Arch. 566 eArchT 3.0 ArchT with addition of trafficking signal from...3.0 Arch with addition of trafficking signal from Kir2.1 566 ArchT Light-activated outward proton pump from...used to inhibit neurons in optogenetic experiments. Arch is a light-activated outward proton pump that hyperpolarizes...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...

...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic...chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant ...eukaryotic, as well as common promoters used in research check out our promoters reference page . Selectable...information to aid in the reproducibility of scientific research. Resources Genetic Code The genetic code can be...newest breakthroughs in plasmid technologies and research, to overviews of molecular biology basics and ...

...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert...Figure 5: Overview of base editing More recently, researchers have developed base editors that can catalyze...