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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7 results for: Arch-GFP

Showing: 1 - 7 of 7 results

  1. Adenovirus Guide

    Type
    Guide
    ...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...recombinant adenoviral vector production that contain IRES-GFP. Pseudotyping The production of viral vectors (or...adenoviral vector production that do not contain GFP. Recombinant adenoviral vector (rAdV) Modified form...Wild-type adenoviruses have been modified by researchers to create recombinant adenoviral (rAdV) vectors...gene expression patterns that were observed, researchers have tended to favor using first- and third-generation...is still the most commonly used generation for research purposes. The system consists of two plasmids ...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell ...treatments into cells, and has been widely used in the research community for decades. Wildtype gamma-retroviruses...vectors have many aspects that make them useful research tools in a variety of cell types and models, such...integration, gamma-retroviral vectors are useful research and clinical tools thanks to their long-term expression...practices with regard to gamma-retroviral-related research. See Addgene’s Biosafety Resource Guide for more...Retroviral Vectors and Their Uses in Scientific Research Gamma-retroviral Packaging Systems The Retroviral...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead of ... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...treatments into cells, and has been widely used in the research community for decades. Wild-type lentiviruses ...vectors have many aspects that make them useful research tools in a variety of cell types and models. Lentiviral... of a transgene, lentiviral vectors are useful research and clinical tools. Stable Cell Lines Lentiviral...best practices with regard to lentiviral-related research. See Addgene’s Biosafety Resource Guide for more...accessibility (DIVA) identifies alterations in chromatin architecture through large-scale mapping of lentiviral integration...

  4. Optogenetics Guide

    Type
    Guide
    ...cell when triggered by green-yellow light. Arch Variants. Arch variants have been developed with the following...Browse Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense is ...589 Archaerhodopsins Arch Light-activated outward proton pump from Halorubrum sodomense 566 eArch 3.0 ...TP009. Improved light sensitivity over Arch. 566 eArchT 3.0 ArchT with addition of trafficking signal from...3.0 Arch with addition of trafficking signal from Kir2.1 566 ArchT Light-activated outward proton pump from...used to inhibit neurons in optogenetic experiments. Arch is a light-activated outward proton pump that hyperpolarizes...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...

  5. Modular Cloning Guide

    Type
    Guide
    ...a combinatorial library of constructs expressing GFP at 18 levels for rapid characterization in new organisms...MoClo Plasmid Kits Available from Addgene Browse or search the table below to find MoClo kits in our collection...Johannes Stuttmann 95 additional plasmids for hierarchical assembly of plant multigene constructs. MoClo...characterized parts that can be used for bottom-up hierarchical assembly of single and multi-gene constructs...

  6. Molecular Biology Reference

    Type
    Guide
    ...contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of the genetic...chromosomal DNA. Although they can be found in archaea and eukaryotes, they play the most significant ...eukaryotic, as well as common promoters used in research check out our promoters reference page . Selectable...information to aid in the reproducibility of scientific research. Resources Genetic Code The genetic code can be...newest breakthroughs in plasmid technologies and research, to overviews of molecular biology basics and ...

  7. CRISPR Guide

    Type
    Guide
    ...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... help you get started using CRISPR in your own research. CRISPR Basics In this section, we will introduce...same chromosome. Most multiplex systems enable researchers to target anywhere from 2–7 genetic loci by cloning...later in the guide. Increasing fidelity of Cas9 Researchers have also enhanced specificity by engineering... overcome the CRISPR systems that bacteria and archaea use to protect against these foreign nucleic acids...Base Editing To overcome low HDR efficiency, researchers have developed base editors to specifically convert...Figure 5: Overview of base editing More recently, researchers have developed base editors that can catalyze...
Showing: 1 - 7 of 7 results