...the host genome via the viral proteins packaged during production (read our blog post on the retroviral...time, IDLVs are ideal. As the episomes are lost during cell division, the expression of the delivered ...activity yet fail to integrate viral DNA efficiently during infection. Journal of Virology, 70(2), 721–728....

...not need to use a separate fix/perm cytosolic procedure in that case.     Figure 3: For intracellular... used. These dyes are incorporated into the DNA during DNA synthesis and then diluted with subsequent ...negative live cells can still be easily gated on during the analysis. Conclusion Congratulations! You made...

...progress), and I know that I’ve blocked out time during my day according to my priorities, I have a pretty...-world example, let’s say I’ve blocked out time during my day to write a blog post, edit other blog posts...leave your phone in your bag on “Do Not Disturb” during the times you’ve blocked out for other activities...

...then used to measure the frequency of each variant during the selection process. Each variant is assigned...then both strands of the plasmid will be nicked during the first BbvCI digest and the whole plasmid will...direction, both strands of the plasmid will be nicked during the first BbvCI digest and the whole plasmid will...

...and plasmid backbone. Because you lose some DNA during the gel purification step, it is important to digest...manufacturer to ensure that you’re cutting for the proper duration and using the proper conditions. Pro Tip! If you...migration speeds; after running the gel for some duration of time, these differently sized pieces will be...

...internationalization and there is a lack of expert mentorship during undergraduate and graduate studies. Students also...Valencia, Spain) as the most improved trajectory during the pilot, and Joaquín López (MIT, USA) as most...experience, and new professional capacity generated during this process result directly in positive impacts...

...that are generated (while talking or pipetting) during working in the laminar airflow are also among the...aerosols generated will enter the culture medium during subculturing processes or from the air if the cell...drawbacks while considering this treatment as the duration of treatment can affect the cell growth and viability...

...normal!  Action Items:  Start a career journal during your current job and note what makes your heart...writing and presenting for non-scientist audiences during your time in graduate school through outreach organizations...programs can connect you with alumni before and during your application process so you can learn more!...

...minimize recombination that are typically employed during viral plasmid amplification (Fischer, KB. 2019)... showing the mechanism of spontaneous reversion during AAV plasmid amplification. On the left homologous...  Some classes of neurons transiently express TH during development or have low levels of TH mRNA without...

...world of academic research can be quite daunting. During my first year of college, I knew I wanted to try... provided me with much-needed emotional support during my journey. When you are hunting for labs to join... and that you want to travel in new directions. During my grad school interviews, one of the professors...

...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells over-express...trials going amiss and patient deaths have occurred during CAR-T cell tests because treatment regimens were... likely to be positively impacted by the trial. During clinical trials, expansion of participants based...

...to double-stranded, and finally, drop once more during a final temperature increase. If there is a single.... Flipping your experimental and control values during analysis can allow you to see this fold change ... as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process. Sci Rep 8, 8716 (2018...

...ourselves accosted by dozens of tiny black dots during our stay there. Yet capturing the flies is nearly...everywhere find and re-contain any specimens that escape during routine lab activities.  Here, we demonstrate the...

... of medical school. 7:05 - 10:55 : Alex's focus during graduate school - DNA damage and longevity. 10:...repair informed his decision to work with CRISPR during his postdoc 12:01 - 13:21 : The Wyss Institute ...

...just over a year old, and it has grown steadily during 2014. We were excited to have more and more scientists...Margo R. Monroe Best wishes for your research during this holiday season and we look forward to seeing...

...coactivator induced in skeletal muscle by exercise and endurance, protects the brain from depression. Mice that...metabolites that are dynamically secreted by muscle during exercise to meet the brain’s physiological demands...

...sciences, I knew I would run into some challenges during my internship, but nothing could have prepared ...challenges would be from doing a remote internship during a pandemic. Before I knew how bad COVID was getting...

... from the software folks and started Hack Days. During Hack Days, teams at Addgene will forgo their regular...website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success at ...

...academic scientists. Expanding plasmid sharing during COVID-19 Realizing this limitation, we worked closely...setting.  In light of the need for basic research during the current pandemic, universities are showing ...

...chromosome. One of the main causes of degradation during plasmid DNA extraction and purification from E....though in their capacity to maintain DNA stability during storage over prolonged periods of time, especially...