We narrowed to 753 results for: ELP
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TypeBlog Post...the bioscience and tech transfer communities who help guide and promote Addgene in its mission to make... Institute and has worked with many Addgenies to help improve the understanding, curation, and explanation...recently sat down with Dr. Doench for an interview to help further introduce him to the Addgene community. ... was barely understood.” Doench’s graduate work helped expand our understanding of how miRNAs regulate...his work at the Broad Institute today, where he helps researchers in a variety of fields develop RNAi ...
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Selecting Your Plasmid Purification Kit
TypeBlog Post...We have outlined some important considerations to help you choose the plasmid prep kit that is right for... significant source of waste in laboratories. To help combat this, some manufacturers have designed eco-friendly...for another time, however!) Where can you look for help? All manufacturers provide specs, protocols, and...purification, reading the full instruction manual will help explain the rationale behind each step and provide...troubleshooting advice. In these cases, it can be helpful to select a kit with easy-to-follow instructions...reviewing their quality assurance documentation can help you select a kit. So...which kit should you pick... -
Antibodies 101: Flow Cytometry Controls
TypeBlog Post...color controls are used to help you set up your voltages and can also be helpful for compensation. You should...isotypes can help you more easily gate your target population. In addition, the controls will help you save... flow cytometry, you’ll need various controls to help you set up and analyze your samples. While you are... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...SARS-CoV-2 plasmids to help academic and industrial labs accelerate research and to help find a faster solution... page on our website. Contributing your results helps other researchers using these plasmids and will ...have been instrumental in achieving our mission to help science move faster in the middle of a global pandemic... the reach of these plasmids is indeed global. Helping scientists speed up research is what we’re here... -
How to Keep a Lab Notebook for Bioinformatic Analyses
TypeBlog Post...outline what works for me, and I hope that it is helpful to those of you just getting started. The practices...clearly stated, specific goal for each analysis will help you locate relevant information in the future. ...brief overview of the approach that you will take to help you plan your analysis. There is no need to go into...run. This technique leaves a trail that further helps you keep track of your exact changes. Alternatively...useful for those that are just starting out, and helps clarify the role of the code. # This is a comment... -
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur
TypeBlog Post...of work you put into it.” Have any other helpful tips to help transition a project from academia to a ...detect unwanted bacteria. How did graduate school help prepare you for the roles and skills necessary to...success in this business. A good presentation can help win a business plan competition and advance investors... its goals and vision for the company; this will help avoid future complications and potential conflicts... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog Post...thinking “How can I help?” and not “What can you do for me?” One of the easiest ways to help is to connect ...build or use a network. Total strangers can’t really help or refer you. Do not ask people you don’t know for...people that want to meet or that you think might be helpful to each other. This is a quick way to do an easy...at that organization. This type of connection can help you gather information, make introductions, and ... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...with Teytelman, Schwessinger joined this cause and helped by expanding this team, leading monthly calls, ...effort later in the year and in the fall of 2018, I helped organize a workshop for community scientists at...tracking material downloads. This was particularly helpful for securing funding to support this effort. The... the team has grown to include experts who could help provide new materials and reproducibility tools....April Clyburne-Sherin, Benjamin Schwessinger for helpful conversations on the history of the group, lessons... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...analogues in nature. For example, helper plasmids were developed to replace helper viruses in AAV production ... entice their hosts to keep them around and even help them spread. They can be inherited by daughter cells...typically also carry the necessary resistance genes to help cells survive their own secretions. Pro tip! Historically...virulence plasmids! This category includes genes that help bacteria invade hosts, including attaching to host... -
6 Tips for Grant Writing
TypeBlog Post...collaborators can also help you get some critical insight into what you're doing wrong. All this helps to improve ...world for your research to rapidly progress. Need Help Building Your Scientific Network? Check out Our ...people to keep contributing to science, it would be helpful for them to know what we are doing and why. I always...grant for a proof-of-principal study were very helpful in assisting me with applications for much larger... -
Deep Dive: qPCR
TypeBlog Post...be its own deep dive, but here are some general helpful principles (and I recommend Bustin, 2017 for a ...literature, a geNorm study (Vandesompele, 2002) will help you select the appropriate reference genes - and...reliable and reproducible data. Here are some tips to help you in your execution: Accuracy is not as important...Setting up triplicates in multiple configurations can help you identify pipetting or evaporation errors. And...occur. A passive reference dye, such as ROX, can help normalize for that. Passive reference dyes are inert...automatically record fluorescent data on all channels - helpful if you forget to set the channel wavelength before... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...useful. We’ll need something like Google Maps to help us identify and visualize addresses. That’s where...where enhancers and genome math come in. They help us to transform raw genomic coordinates into meaningful...difference. That’s the intuition for how genome math helps us to locate interesting addresses in the genome...powerful stretches of DNA known as enhancers with the help of genome math. Figure 1: Genome math illustrated...yet to explore. I hope that this brief guide can help you do just that. Good luck, and always keep going... -
A Career in Grants: How To Become a Grant Professional
TypeBlog Post...listed below can give you the resources and path to help you enter and to have a successful and meaningful...passion for the arts? Do you find satisfaction in helping your local food shelf raise money? Do you have ...consultant, building your area of expertise will help you find work and target your professional development...and coaching services, including Smartegrants, to help those entering the Grants Profession. Whether you...first career, a mentor in the Grants Profession can help you focus your career goals, identify your niche... -
Transferable Skills Guide: External Collaborations
TypeBlog Post...offering advice on areas where you might need some help. Today in our transferable skills guide: Collaborating...to respond in a supportive way and are willing to help because you are not (yet) asking for a job or a ...outside my university approachable, friendly, and helpful. Finally, don’t forget about collaborations and...land you a job someday. Universities themselves can help you do this through practical tools like alumni ...organizations in science and beyond. For example, I help organize scientific workshops, manage conference... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog Post...the hopes that these findings can help others as much as they've helped us. Interested in providing feedback... to ensure that the service has grown steadily, helped by a partnership with the University of Pennsylvania...broadest category of viral vector tools, which may help explain why they are the most commonly requested...Browsing the Data Hub before selecting an AAV can help a researcher make a more informed choice on which... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...Understanding the function of these genes in fish will help clarify their roles in humans. The sequenced genome...Additional analysis tools have been developed to help parse and visualize expression data in useful ways...mutagenesis screens. Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those ...cell types. Sequencing-based lineage tracing can help elucidate the gene networks that transform zygotes...this field and others are likely to continue with help from zebrafish. Many thanks to our guest blogger... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post...vocabulary for this skill. What this post may do is help you realize whether or not you have a natural inclination...resolve conflicts within a given organization, it can help tremendously to have as much context as possible... conflict resolution? Definitely not. But it can help, and if and when you find yourself transferring ...you are able to broker a compromise well, you can help everyone walk away from a conflict happy, feeling... the workplace is inevitable, but having who can help resolve these conflicts is not inevitable. When ... -
10 Steps to a Perfect Science Talk
TypeBlog Post...added bonus to knowing your audience is that it can help you avoid making mistakes. I once made a joke about...doing it for the first time? Practice talks also help a speaker determine how long the talk will really...clear and concise answers. This preparation will help you avoid giving only yes or no answers in front...ready after your formal conclusion slide will also help with your confidence. It is best not to be asked...the sharing model works. See if you can elicit helpful comments from your audience as in “Does anyone ... -
Finding Your Perfect Job After University
TypeBlog Post... a tremendously positive experience. I enjoyed helping people and learning to explain concepts in ways...from my role in Industry and took evening work to help cover the costs. My MSc courses were interesting...my idea of a dream job, but I knew that I enjoy helping people and decided to compromise and applied anyway...job. Because of my background in genetics, I can help scientists problem solve experiments over the phone...email me are scientists who are grateful for some help. I also get the chance to contribute content to... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...obstacles; for instance there are few resources to help get individuals started on the path to internationalization...talented professionals to their original countries to help build, together with the universities, a bright ...level where I would like to be in a few years, helping me to advance in my career.” Adrian Mancebo (Mentee...discussion topics at IMP In these sessions, mentors helped mentees create “Career Development Plans” which...goals, and identified possible funding sources to help them carry out their plans. Through their shared...