We narrowed to 143 results for: Endog
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TypeBlog Post...expressed in the neuron, the FingRs bind to the endogenous synaptic proteins allowing them to localize to...
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Viral Vectors 101: Calcium Sensors
TypeBlog Post... prevent aberrant binding of the biosensor to endogenous calmodulin. Multiplexing Are you combining your... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...protocols that use antibody fragments to block the endogenous immunoglobulins and reduce the background staining... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...your protein of interest. TIR1 interacts with endogenous Skp1 and Cul3 proteins to form a SCF E3 ubiquitin... -
Plasmids 101: FLEx Vectors
TypeBlog Post...mutations and truncations in the absence of the endogenous gene. Spatio-temporal expression of optogenetic... -
Chemogenetics Guide
TypeGuide...respond to small molecules that do not affect endogenous signaling, therefore allowing precise control... mutated receptors that while unresponsive to endogenous ligands, could be activated by synthetic ligands...receptors, but unlike RASSLs, show insensitivity to endogenous ligands, have low constitutive activity, and ... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog Post...Western blotting and PCR, depending on the level of endogenous expression. We highly recommend the use of an... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post...the Addgene Blog Learn More about Plasmids for Endogenous Gene Tagging in Human Cells Check Out the Michael... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...CAPTURE CAPTURE 2.0 Biotinylation enzyme BirA Endogenous biotin ligases Expression system Stable cell... -
Hot Plasmids: Summer 2025
TypeBlog Post...) for easy visualization. A cleavage site for endogenous BACE1 protease allows VAMP2-At to remain on the... -
Of Myc and Men
TypeBlog Post...impossible if you wanted to distinguish between endogenous and exogenous versions of the same target. In... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog Post... level observed will be dependent on both the endogenous promoter and the 3’ UTR of the locus of interest... -
CRISPR 101: RNA Editing with Cas13
TypeBlog Post...then tested the RESCUE system’s ability to edit endogenous transcripts finding up to 42% editing efficiency... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog Post...from a template molecule, which is usually an endogenously available sister chromatid, though exogenous... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...new effector (Abudayyeh et al., 2016). In the endogenous CRISPR/Cas9 system, Cas9 uses both a tracrRNA... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post...Agrobacterium lacking the GUS gene since plants can have endogenous GUS-like activity (Hu et al., 1990). We commonly... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog Post... this using Cre-Lox mouse models in which the endogenous MECP2 gene was disrupted and silenced by a Lox-Stop... -
To Codon Optimize or Not: That is the Question
TypeBlog Post...efficiency is correlated with codon bias across all endogenous genes in E. coli and S. cerevisiae (Tuller et... -
CRISPR-mediated Plant Base Editors
TypeBlog Post...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system." Proceedings of the National... -
CRISPR Activation: A Practical Guide
TypeBlog Post...light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11:198–200 . https...