We narrowed to 327 results for: INO

1. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...gRNAs used the RNA nuclease Csy4 from Pseudomonas aeruginosa to cleave 4 gRNAs separated by 28 base Csy4 recognition...processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part of their CRISPR...

2. CRISPR 101: Non-Homologous End Joining

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   ...Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) ...

3. Plasmid Tools for Microbiome Studies

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   ...under control of the arabinose-inducible promoter. Cells are first induced with arabinose to begin expressing...

4. Antibodies 101: Conjugation

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   ... antibodies, such as inclusion of non-canonical amino acids or peptide tags. 5) Strong protein-protein...Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for Conjugation of Effector Molecules...

5. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...common tags currently used, are approximately 240 amino acids in size. The size of these proteins, or dimerization...membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin of the influenza...

6. Career Coaching for Scientists: Why and Where Do I Find One?

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   ...is also a group The Dissertation Coach based in Illinois. A few of the coaches in this group have scientific... to mentoring for women and under-represented minorities. Her online content is of great value. I have...

7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   ...under certain conditions. With its additional 9 amino acid peptide, the CRY2clust domain enables fast ... glutathione S-transferase homolog from a Janthinobacterium sp. Moreover, a caffeine “addicted” E. coli...

8. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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   ...of college as a biochemistry major and a Spanish minor, it’s time for me to start considering my future... Addgenie Shreya Vedantam with our resident dinosaur! Read her Instagram feature here. One of our...

9. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   ... in N-terminal fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput...Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et al. Improved split fluorescent proteins ...

10. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ...” column. Relevant Mutations Indicate the amino acid change as well as any functional consequences...hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of...

11. Viral Vectors 101: Calcium Sensors

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    ...Nature chemical biology. 2021 May;17(5):509-18. Inoue M. Genetically encoded calcium indicators to probe...Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino M. Genetically encoded fluorescent indicators for...

12. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ... the amino acid sequences of the antibodies and then synthesize the genes that encode those amino acids...

13. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...for sequencing, we feel confident that these few minor mismatches usually don’t affect the function of ..., we check to see if they affect the translated amino acid. We also confirm that the species of the gene...

14. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...serious adverse effects, and their immunogenic or carcinogenic profiles have limited their usability in clinical...

15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    ...farmers markets), and serious ideas (how to stop rhino horn poaching using biotechnology) were thrown on...Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted ...

16. Plasmids 101: Protein Expression

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    ...ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems are ideal for... to assemble proteins with labelled or modified amino acids that are useful in different downstream applications...

17. Plasmids 101: Optimizing Plasmid Yields

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    ...often doubles the volume of culture used for inoculation if we get inadequate plasmid DNA yields. Why ...growth conditions, and the material used for inoculation. To further complicate matters, plasmid copy ...

18. Plasmids 101: Multicistronic Vectors

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    ...discovered in picornaviruses, are short (about 20 amino acids) and produce equimolar levels of mulitple ... type or experimental conditions.   Peptide  Amino acid sequence* T2A: (GSG) E G R G S L L T C G ...

19. DNA Quantification

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    ...Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering Plasmid...

20. Tips for arabidopsis transformation

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    ...of culture you are using and whether you are inoculating from a frozen stock or a colony from an LB plate...plate. In my hands, a 5 mL starter culture inoculated from a colony on a plate will reach an optimal density...