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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 326 results for: INO

Showing: 101 - 120 of 326 results

  4. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...Nature chemical biology. 2021 May;17(5):509-18. Inoue M. Genetically encoded calcium indicators to probe...Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino M. Genetically encoded fluorescent indicators for...

  7. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...serious adverse effects, and their immunogenic or carcinogenic profiles have limited their usability in clinical...

  9. TALEN Expression Vectors

    Type
    Collection
    ...TAL effector repeat arrays with identical DNA and amino acid sequences (i.e. only the details of the assembly...epitope tag and nuclear localization signal at their amino-terminus. The expression vectors listed below can...

  10. Brain Initiative Collection

    Type
    Collection
    ...for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable...for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable...

  11. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...RNA editor that converts adenosine to inosine in RNA. Since inosine is functionally equivalent to guanosine...

  12. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems are ideal for... to assemble proteins with labelled or modified amino acids that are useful in different downstream applications...

  13. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...often doubles the volume of culture used for inoculation if we get inadequate plasmid DNA yields. Why ...growth conditions, and the material used for inoculation. To further complicate matters, plasmid copy ...

  14. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...discovered in picornaviruses, are short (about 20 amino acids) and produce equimolar levels of mulitple ... type or experimental conditions.   Peptide  Amino acid sequence* T2A: (GSG) E G R G S L L T C G ...

  17. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...(ADAR). Such a system would convert adenine to inosine, which the translational machinery treats like ... ADAR1 and ADAR2, which deaminate adenosine to inosine in RNA, creating a functional A->G change. They...

  18. Tips for arabidopsis transformation

    Type
    Blog Post
    ...of culture you are using and whether you are inoculating from a frozen stock or a colony from an LB plate...plate. In my hands, a 5 mL starter culture inoculated from a colony on a plate will reach an optimal density...

  19. CRISPR Antimicrobials

    Type
    Blog Post
    ...Figure 1). Citorik et al. targeted beta-lactam and quinolone resistance genes in E. coli using plasmid and ...plasmid. In the case of the genomically encoded quinolone resistance, mediated by a single base pair change...

  20. Plasmids 101: Protein tags

    Type
    Blog Post
    ...affinity tag, generally a relatively small sequence of amino acids, is basically a molecular leash for your protein...; good for antibody-based detection SUMO ~100 amino acid protein  12 Stability At N-terminus, promotes...
Showing: 101 - 120 of 326 results