We narrowed to 322 results for: INO

...conditions aren't working against you!  The culture inoculum Freshly streaked bacteria have higher copy numbers...versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia coli. Biochemistry...

...studies showed that iTOP functions through macropinocytosis. D’Astolfo et al. tested iTOP in a series... cultured mammalian cells by osmotic lysis of pinocytic vesicles. Okada CY, Rechsteiner M. Cell. 1982 ...

...is induced prior to inoculation with IPTG. In the absence of IPTG after inoculation, no more fluorescent...

...recombinases, based on sequence similarity and the amino acid residue used in the active site. PhiC31 integrase...

...best known: it’s derived from the small (19 kDa) luminous shrimp (Oplophorus gracilirostris) luciferase ...Luciola cruciata) Natural 562 64 Luciferin ATP No Luminous shrimp(Oplophorus gracilirostris) Natural 455 ...

...only requires a small epitope tag (FLAG is eight amino acids, HA is nine), multiple repeats of the tag ...editing and gene activation, is composed of only 529 amino acids, less than half the size of either Cas9 or...

...a small peptide that represents a short, unique amino acid sequence found in the protein, or, are injected...a single epitope, so it’s less likely that this amino acid sequence will be shared across other proteins...

...conservation, or predicted deleteriousness of the amino acid change based on maps of protein structure. ...cancer variants derived from analysis of lung adenocarcinoma genomes (Lung Cancer Mutant Collection) or ...

...gRNAs used the RNA nuclease Csy4 from Pseudomonas aeruginosa to cleave 4 gRNAs separated by 28 base Csy4 recognition...processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part of their CRISPR...

...Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) ...

...common tags currently used, are approximately 240 amino acids in size. The size of these proteins, or dimerization...membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin of the influenza...

... antibodies, such as inclusion of non-canonical amino acids or peptide tags. 5) Strong protein-protein...Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for Conjugation of Effector Molecules...

...under control of the arabinose-inducible promoter. Cells are first induced with arabinose to begin expressing...

...is also a group The Dissertation Coach based in Illinois. A few of the coaches in this group have scientific... to mentoring for women and under-represented minorities. Her online content is of great value. I have...

...Quantification DNA Quantification You may also like... Inoculating a Liquid Bacterial Culture Recovering Plasmid...

... in N-terminal fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput...and Resources Reference Feng, S., Sekine, S., Pessino, V. et al. (2017) Improved split fluorescent proteins...

... the amino acid sequences of the antibodies and then synthesize the genes that encode those amino acids...

...for sequencing, we feel confident that these few minor mismatches usually don’t affect the function of ..., we check to see if they affect the translated amino acid. We also confirm that the species of the gene...

...Nature chemical biology. 2021 May;17(5):509-18. Inoue M. Genetically encoded calcium indicators to probe...Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino M. Genetically encoded fluorescent indicators for...

...of college as a biochemistry major and a Spanish minor, it’s time for me to start considering my future... Addgenie Shreya Vedantam with our resident dinosaur! Read her Instagram feature here. One of our...