We narrowed to 319 results for: INO

1. CRISPR 101: Non-Homologous End Joining

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   ...Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) ...

2. Zebrafish Plasmid Collection

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   ...transposons, Cre-mediated site-specific recombination, morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9...Gateway destination vectors (pGCS), bearing either amino- or carboxyl-terminal tags, including Myc, HA, Flag...

3. University of Florida Serotype Testing Panel for the Eye and Brain

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   ...Recombinant Adeno-Associated Virus Vector Expressing Retinoschisin. Hum Gene Ther Clin Dev . Sep;26(3):165-76. .... PMID: 26390090 Other citations include: Scalabrino, et al. 2015. Intravitreal delivery of a novel AAV...

4. Antibodies 101: Conjugation

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   ... antibodies, such as inclusion of non-canonical amino acids or peptide tags. 5) Strong protein-protein...Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for Conjugation of Effector Molecules...

5. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...common tags currently used, are approximately 240 amino acids in size. The size of these proteins, or dimerization...membrane. The haemagglutinin (HA) tag is a small 9 amino acid epitope of the haemagglutinin of the influenza...

6. Plasmid Tools for Microbiome Studies

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   ...under control of the arabinose-inducible promoter. Cells are first induced with arabinose to begin expressing...

7. Career Coaching for Scientists: Why and Where Do I Find One?

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   ...is also a group The Dissertation Coach based in Illinois. A few of the coaches in this group have scientific... to mentoring for women and under-represented minorities. Her online content is of great value. I have...

8. Viral Vectors 101: Calcium Sensors

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   ...Nature chemical biology. 2021 May;17(5):509-18. Inoue M. Genetically encoded calcium indicators to probe...Research. 2021 Aug 1;169:2-8. Suzuki J, Kanemaru K, Iino M. Genetically encoded fluorescent indicators for...

9. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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   ...” column. Relevant Mutations Indicate the amino acid change as well as any functional consequences...hygromycin, kanamycin, nourseothricin (clonNat), spectinomycin, streptomycin, tetracycline, combinations of...

10. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

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    ... the amino acid sequences of the antibodies and then synthesize the genes that encode those amino acids...

11. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

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    ...for sequencing, we feel confident that these few minor mismatches usually don’t affect the function of ..., we check to see if they affect the translated amino acid. We also confirm that the species of the gene...

12. Meet an Addgenie: Insights into Grad School, Career Paths, and More

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    ...of college as a biochemistry major and a Spanish minor, it’s time for me to start considering my future... Addgenie Shreya Vedantam with our resident dinosaur! Read her Instagram feature here. One of our...

13. Adeno Associated Virus (AAV) for Cell and Gene Therapy

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    ...investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles. These technologies...serious adverse effects, and their immunogenic or carcinogenic profiles have limited their usability in clinical...

14. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...under certain conditions. With its additional 9 amino acid peptide, the CRY2clust domain enables fast ... glutathione S-transferase homolog from a Janthinobacterium sp. Moreover, a caffeine “addicted” E. coli...

15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ... in N-terminal fragment An enhanced recombinant amino-terminal acetylation system and novel in vivo high-throughput...Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et al. Improved split fluorescent proteins ...

16. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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    ...farmers markets), and serious ideas (how to stop rhino horn poaching using biotechnology) were thrown on...Molecular Biophysics and Biochemistry from the Illinois Institute of Technology in 2004, she accepted ...

17. Rinehart Lab Phosphoprotein Reagents

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    ...serine phosphorylation by adding this phosphorylated amino acid to the genetic code of E. coli (Park et al....consist of a central phosphoserine site flanked by 15 amino acids on either side occurring within the parent...

18. TALEN Plasmids and Kits

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    ...determined by customizable arrays of polymorphic amino acid repeats in the TAL effectors. View Addgene'...DNA-binding ability of TALEs), 63 wild-type TAL amino acids after the repeat domain, and C-terminal SV40...

19. Plasmids 101: Protein Expression

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    ...ribosomes, RNA polymerase, tRNAs, ribonucleotides and amino acids. Cell-free expression systems are ideal for... to assemble proteins with labelled or modified amino acids that are useful in different downstream applications...

20. Plasmids 101: Optimizing Plasmid Yields

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    ...often doubles the volume of culture used for inoculation if we get inadequate plasmid DNA yields. Why ...growth conditions, and the material used for inoculation. To further complicate matters, plasmid copy ...