Skip to main content
Addgene
Showing: 101 - 120 of 235 results

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...Technologies. The MultiSite Gateway Kit is a customizable Gateway®-based toolkit that allows scientists to generate... variety of model systems. The MultiSite Gateway Kit contains a set of 12 promoter-less Gateway® entry...building block for further chaining. The MXS-chaining kit contains 5 empty chaining vectors plus a destination...Testing your cell-free expression system: There’s a kit for that! Cell-free expression systems have been...expression systems; the Cell-free expression test kit represents a set of 88 clones in a pCellFree vector... et al., Biotechnol Lett. 2015. 37:383-389. Toolkit for constructing fusion proteins with customizable...tags. The Santalucía Lab has developed a cloning toolkit based on the Multisite Gateway® technology from...

  8. Antibodies 101: ChIP

    Type
    Blog Post
    ...Finally, purify your DNA with a PCR purification kit or with a phenol-chloroform extraction.    ...

  11. Adenovirus Plasmids

    Type
    Collection
    ...in a one-tube reaction. ID Kit Description PI 1000000176 AdenoBuilder toolkit Plasmids contain modular ... 62622 for plasmid without Venus Wang Adenoviral Kits Plasmids containing modular inserts that span the...

  15. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma #A9414...for 2 hours. Purify with Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Digest eluate with EcoRI.... Purify the DNA using a Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA concentration...Materials Material Vendor and catalog # DNA Miniprep Kit Qiagen #27104 EcoRI NEB #R0101S NcoI NEB #R0193S ... 2: 2. Spin down the cultures and use a miniprep kit to obtain DNA. 3. Conduct a restriction digest with...

  16. Addgene: The First Twenty Years

    Type
    Blog Post
    ...for pcDNA-p160MBP, while the first request for a kit came on March 9, 2005. It took almost six years to...

  18. Bacterial Expression Systems

    Type
    Collection
    ...aid disulfide bond formation F Xavier Gomis-Ruth Kit containing plasmids with a wide variety of N and ...BIOFAB plasmid set 1000000037 N/A N/A Drew Endy This kit contains plasmids with various combinations of constituitive... Consortium: Modular Assembly Accessory Reagents Kit Various Plasmids Sequence chosen by researcher Beta-galactosidase...Beta-galactosidase Keith Joung Transcription factor testing: Kit contains five plasmids and one bacterial strain for...

  19. 6 Tips for Grant Writing

    Type
    Blog Post
    ... the entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great ...

  20. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen...the meeting, in addition to useful plasmids and toolkits available at Addgene.  C. elegans open resources... C. elegans meeting. Here I’ll highlight a few toolkits that we have available at Addgene that were discussed...Jorgensen lab. SapTrap is a reagent toolkit and plasmid assembly line for the creation of CRISPR targeting...Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput CRISPR/Cas9 gene modification...
Showing: 101 - 120 of 235 results