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Addgene

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We narrowed to 13 results for: KIT

Showing: 1 - 13 of 13 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...bacterial cultures. If you will be using a kit, follow the kit's instructions. If you want to perform plasmid...plasmid purification without using a kit, you can find a protocol for kit-free plasmid mini-prep at the bottom... , and (Link opens in a new window) Promega sell kits for isolating plasmid DNA in quantities as low as...concentration and purity click here . Top Protocol: Kit-free Alkaline Lysis Plasmid Miniprep Prepare the ...Store DNA at 4°C. Tips and FAQ Plasmid purification kits provide the fastest way to obtain a high concentration...improve the purity of plasmid DNA purified without a kit it is advisable to perform a phenol/chloroform extraction...This is included in the resuspension buffer of most kits. Reference Page Top Index...

  2. Kit Free RNA Extraction

    Type
    Protocol
    ... Protocols RNA Extraction Without a Kit RNA Extraction Without a Kit You may also like...extraction without a kit. Protocols... like... Kit-Free DNA Purification Agarose Gel Purification Molecular Biology Reference Introduction This...how to extract RNA from tissues or cells without a kit. The general steps of this protocol include homogenization... read this blog post on RNA extraction without a kit . Last Update: April 10, 2020 Equipment Refrigerated...

  3. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ... purification kit, such as the (Link opens in a new window) QIAquick Gel Extraction Kit . Always follow...important during the DNA purification step, as many kits cannot handle more than a certain total volume of...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...cells Invitrogen #18258-012 Qiaquick gel extraction kit Qiagen #28704 Low melting point agarose Sigma #A9414...for 2 hours. Purify with Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Digest eluate with EcoRI.... Purify the DNA using a Qiaquick gel extraction kit. Elute in 30 μL of ddH 2 O. Measure the DNA concentration...Materials Material Vendor and catalog # DNA Miniprep Kit Qiagen #27104 EcoRI NEB #R0101S NcoI NEB #R0193S ... 2: 2. Spin down the cultures and use a miniprep kit to obtain DNA. 3. Conduct a restriction digest with...

  8. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...tetramethyl-[1,1'-biphenyl]-4,4'-diamine) substrate kit, Pierce 34021 Tween-20, VWR 0777-1L Foil Before Starting...and 5 mL Peroxide Solution from the TMB substrate kit. Cap the tube and invert several times to mix. Using...

  10. Lentivirus ddPCR Titration

    Type
    Protocol
    ...10199-452 Reagents GeneJet Genomic DNA Purification Kit, Thermo Fisher, K0721 6-well tissue culture treated...according to the GeneJet Genomic DNA Purification Kit instructions. Determine the concentration of each...

  11. Gibson Assembly Protocol

    Type
    Protocol
    ... (Link opens in a new window) sells DNA Assembly kits and master mixes, including NEBuilder HiFi (Link...

  12. Western Blot

    Type
    Protocol
    ...the protein concentration using a Pierce BCA Assay Kit or other preferred method for protein determination...

  13. CRISPR Library Amplification

    Type
    Protocol
    ...Purify plasmid DNA using the Qiagen HiSpeed Maxi Kit (one conical is its own Maxiprep). Critical Do not...
Showing: 1 - 13 of 13 results