We narrowed to 208 results for: Kars

1. Viral Vectors 101: What is a Virus (and a Viral Vector)?

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   ...infected cell.) This is in contrast to bacteria and eukaryotes, which use a binary replication process whereby...

2. Hot Plasmids and Viral Preps - September 2021

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   ...previously developed such a system in Streptomyces, a prokaryotic genus capable of producing a variety of natural...

3. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   ..., Giandhari J, Pillay S, Wilkinson E, Naidoo Y, Karim F, Ganga Y, Khan K, Bernstein M, Balazs AB, Gosnell...

4. Another Pathway into Cells: iTOP

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   ...Native Proteins. D'Astolfo DS, Pagliero RJ, Pras A, Karthaus WR, Clevers H, Prasad V, Lebbink RJ, Rehmann H...

5. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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   ...of multiple loci is deftly coordinated by the eukaryotic cell for the execution of many complex cellular...

6. "Build Your Plasmid": The Game - Play to Teach

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   ... students are not even allowed to manipulate prokaryotic cells? Especially, if you only have 4 hours and...

7. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   ...sequence and ultimately its degradation. While other eukaryotes don’t naturally have this response, they do contain...

8. Plasmids 101: Mammalian Vectors

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   ... 100-400 ug/mL *Not comprehensive.   ** In eukaryotes.   ***The concentration used for selection is...

9. Multiplex Genome Editing with CRISPR-Cpf1

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   ... Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1...

10. Addgene’s Viral Service - Why Virus? Why Now?

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    ... our virus. Ed Boyden’s lab from MIT and Alla Karpova’s lab from Janelia Research Campus have provided...

11. Antibodies 101: Monoclonal Antibodies

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    ...https://doi.org/10.1093/ilar.46.3.258  Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ ...

12. mTOR Pathway

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    ...provided below. mTORC1 Complex Symbol Name 4E-BP Eukaryotic translation initiation factor 4E, binding protein...protein 1 Cyclin D1 Also known as CCND1 eIF-4E Eukaryotic translation initiation factor 4E GBL/mLST8 MTOR...

13. AAVED

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    ... School Dr. Melina Fan, Dr. Susanna Bachle, Dr. Karen Guerin, Dr. Leila Haery, Dr. Meghan Rego, Addgene...Campus Kenton Woodard Penn Vector Core Melina Fan, Karen Guerin, and Leila Haery from Addgene will also be...

14. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

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    ...Autophagy. Methods in Enzymology Autophagy: Lower Eukaryotes and Non-Mammalian Systems, Part A, 33-42. doi...

15. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    ...PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine ...

16. Viral Vectors 101: An Introduction to AAV

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    ...Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik. Didem Goz Ayturk is a...

17. Antibodies 101: Affinity Reagents

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    ...monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used as intracellular...

18. Adenoviral Vector Production and Troubleshooting

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    ...  Karen Guerin...

19. Plasmids 101: The Wide World of Natural Plasmids

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    ...bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA passengers....

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...hear it (whether at a scientific conference or a karaoke bar), you probably want to sigh. The problem is...