Addgene

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Showing: 101 - 120 of 207 results

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"Build Your Plasmid": The Game - Play to Teach

Type

Blog Post

... students are not even allowed to manipulate prokaryotic cells? Especially, if you only have 4 hours and...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

...sequence and ultimately its degradation. While other eukaryotes don’t naturally have this response, they do contain...
Plasmids 101: Mammalian Vectors

Type

Blog Post

... 100-400 ug/mL *Not comprehensive. ** In eukaryotes. ***The concentration used for selection is...
Addgene’s Viral Service - Why Virus? Why Now?

Type

Blog Post

... our virus. Ed Boyden’s lab from MIT and Alla Karpova’s lab from Janelia Research Campus have provided...
Multiplex Genome Editing with CRISPR-Cpf1

Type

Blog Post

... Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P., Zhang, F. (2015). Cpf1...
Antibodies 101: Monoclonal Antibodies

Type

Blog Post

...https://doi.org/10.1093/ilar.46.3.258 Mould AP, Askari JA, Byron A, Takada Y, Jowitt TA, Humphries MJ ...
Viral Vectors 101: An Introduction to AAV

Type

Blog Post

...Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik. Didem Goz Ayturk is a...
The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Type

Blog Post

...PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine ...
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Type

Blog Post

...Autophagy. Methods in Enzymology Autophagy: Lower Eukaryotes and Non-Mammalian Systems, Part A, 33-42. doi...
Antibodies 101: Affinity Reagents

Type

Blog Post

...monobodies can be easily expressed in transfected eukaryotic cells, allowing for them to be used as intracellular...
Plasmids 101: The Wide World of Natural Plasmids

Type

Blog Post

...bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA passengers....
Adenoviral Vector Production and Troubleshooting

Type

Blog Post

... Karen Guerin...
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Type

Blog Post

...hear it (whether at a scientific conference or a karaoke bar), you probably want to sigh. The problem is...
Hot Plasmids and Viral Preps - March 2021

Type

Blog Post

...status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed a set of ...
Illuminating Epigenetics with A FRET Based Biosensor

Type

Blog Post

...References 1. Ong-Abdullah, Meilina, et al. "Loss of Karma transposon methylation underlies the mantled somaclonal...
Degrading DNA with Cascade-Cas3

Type

Blog Post

...1477. https://doi.org/10.1038/s41587-019-0310-0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,...
The AAVantages of AAV in CRISPR Screens

Type

Blog Post

...get around this restriction. The most obvious workaround is to use a multi-plasmid system, where you have...
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

Type

Blog Post

...recognized by biotin ligases normally expressed in eukaryotic cells. Using this tag reduces the number of CAPTURE...
Transferable Skills Guide: Managing a Team

Type

Blog Post

... Karen Guerin...
Plasmids 101: Knockout/Knock-In Plasmids

Type

Blog Post

...Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "Overview: Generation of Gene Knockout...

Showing: 101 - 120 of 207 results

Results per page:

"Build Your Plasmid": The Game - Play to Teach

Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Plasmids 101: Mammalian Vectors

Addgene’s Viral Service - Why Virus? Why Now?

Multiplex Genome Editing with CRISPR-Cpf1

Antibodies 101: Monoclonal Antibodies

Viral Vectors 101: An Introduction to AAV

The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

Antibodies 101: Affinity Reagents

Plasmids 101: The Wide World of Natural Plasmids

Adenoviral Vector Production and Troubleshooting

Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

Hot Plasmids and Viral Preps - March 2021

Illuminating Epigenetics with A FRET Based Biosensor

Degrading DNA with Cascade-Cas3

The AAVantages of AAV in CRISPR Screens

Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

Transferable Skills Guide: Managing a Team

Plasmids 101: Knockout/Knock-In Plasmids

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